Effect of Storage at Temperature (4C) on Complete Blood Count Parameters

Abstract

Abstract:Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. Most blood counts today include a CBC count and leukocyte differential count (LDC) (that is, not just the total WBC count but also the count of each WBC type, such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes). More sophisticated modern analyzers can provide extended differential counts. Objective: This study aimed to assess the effect of Storage and Temperature (4C) on Complete Blood Count Parameters. Materials and Methods: This were cross sectional study conducted in Khartoum state in 2016. It included 100 samples, complete blood count conducted to all 100 samples freshly, after 24hours, 48 hours and 72 hours. Mindary BC-2800 hematological analyzer was used to estimate CBC. Results: There was a significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05). Lymphocytes count, Mixed count, Lymphocytes %, Mixed % and PCT did not show statistically difference ant it were insignificant with P. values (P=0.067), (P=0.080), (P=0.072), (P=0.088) and (P=0.060) respectively. Conclusion: This study concluded that storage caused significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05).

Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC

“…The same study by Daves et al (2015) showed significant differences in the number of erythrocytes in human blood samples stored for 6 hours in K2EDTA at 4°C. Different study results were reported by Ali (2017); the number of erythrocytes in human blood samples stored for 72 hours in K3EDTA at 4°C did not show significant differences. The results of another study reported that storing human blood samples for 8 hours in K3EDTA at room temperature did not show a significant difference in the number of erythrocytes (Putri et al, 2019).…”

“…Putri et al (2019) reported a similar study's results that the hemoglobin levels of human blood samples stored for 8 hours in K3EDTA anticoagulant did not show significant differences. The results of the study by Ali (2017) reported that hemoglobin levels in fresh blood samples with K3EDTA anticoagulant at 24, 48, and 72 hours of storage at 4°C did not show significant differences.…”

Study of refrigerated storage of blood at 4°C on automated hematological parameters & morphological changes in peripheral blood smear: A prospective study

“…2 Complete hemogram is also necessary for patients who requires blood transfusion to estimate the quantity of blood to be transfused. 3 The capability of sample to retain the initial value of a measured quantity for defined period within specific limits when stored under defined condition is termed as sample stability. 4 This sample stability is essential to maintain accuracy & reliability of final results in case of delayed evaluation of blood samples.…”