Effect of substrate and culture conditions on the production of amylase and pullulanase by thermophilic Clostridium thermosulforegenes SVM17 in solid state fermentation

Mrudula, S.; Reddy, Gopal; Seenayya, G.

doi:10.21161/mjm.24110

Cited by 7 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The best substrate for xylanase production was wheat bran due to its nutritional content and large surface area [ 29 ], where wheat bran contained xylan and protein, which were served as carbon and nitrogen sources for microorganisms, respectively [ 30 ]. α-Amylase was also exhibited the maximum production by Clostridium thermosulforegenes using wheat bran [ 31 ]. Wheat bran characterized by its better air circulation, loose particle binding and efficient penetration by mycelia and cheaper, therefore it showed a better prospect economically in fermentation processes [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the maximum saccharification content of total soluble carbohydrates (140 mg/g solid) and reducing sugars (55 mg/g solid) was observed at 70 and 80% moisture, respectively (Figure 3 b). Other results showed that the yield of α-amylase and amylopullulanase by Clostridium thermosulfurogenes increased with an increase in solid to moistening agent ratio from 1:0.5 to 1:2.5 (30 to 75%) with a maximum at 1:2.25 (73%) [ 31 ]. Optimum xylanase production from Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium

et al. 2014

View full text Add to dashboard Cite

Back groundFor enzyme production, the costs of solid state fermentation (SSF) techniques were lower and the production higher than submerged cultures. A large number of fungal species was known to grow well on moist substrates, whereas many bacteria were unable to grow under this condition. Therefore, the aim of this study was to isolate a highly efficient strain of Bacillus sp utilizing wheat bran in SSF and optimizing the enzyme production and soluble carbohydrates.ResultsA local strain Bacillus megatherium was isolated from dung sheep. The maximum production of pectinase, xylanase and α-amylase, and saccharification content (total soluble carbohydrates and reducing sugars) were obtained by application of the B. megatherium in SSF using wheat bran as compared to grasses, palm leaves and date seeds. All enzymes and saccharification content exhibited their maximum production during 12–24 h, at the range of 40–80% moisture content of wheat bran, temperature 37-45°C and pH 5–8. An ascending repression of pectinase production was observed by carbon supplements of lactose, glucose, maltose, sucrose and starch, respectively. All carbon supplements improved the production of xylanase and α-amylase, except of lactose decreased α-amylase production. A little increase in the yield of total reducing sugars was detected for all carbon supplements. Among the nitrogen sources, yeast extract induced a significant repression to all enzyme productivity. Sodium nitrate, urea and ammonium chloride enhanced the production of xylanase, α-amylase and pectinase, respectively. Yeast extract, urea, ammonium sulphate and ammonium chloride enhanced the productivity of reducing sugars.ConclusionsThe optimization of enzyme production and sccharification content by B. megatherium in SSF required only adjustment of incubation period and temperature, moisture content and initial pH. Wheat bran supplied enough nutrients without any need for addition of supplements of carbon and nitrogen sources.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Different agricultural residues were used as substrates and among those substrates, wheat bran showed the maximum synthesis of α-amylase by using Bacillus megatherium in SSF. Clostridium thermosulforegenes provided the highest synthesis of α-amylase by utilizing wheat bran (Mrudula et al, 2011). Due to the loose binding of wheat bran particles, air can easily circulate among them.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation

Farooq¹,

Ali²,

Hassan³

et al. 2023

PUJZ

View full text Add to dashboard Cite

show abstract

“…Moreover, SSF has fewer chances of contamination because most contaminants cannot survive in the absence of low water activity. After process optimization in SSF, bacterial species such as Bacillus licheniformis ( Khalaf and Aldeen., 2013 ) and Clostridium thermosulforegenes ( Mrudula et al, 2011 ) produced pullulanase effectively. The feasibility of using SSF to produce the enzyme on a large scale, however, was not investigated ( Akassou and Groleau, 2019 ).…”

Section: Approaches Used To Increase Pullulanase Yieldmentioning

confidence: 99%

Pullulanase: unleashing the power of enzyme with a promising future in the food industry

Naik

Kumar

Goyal

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Pullulanases are the most important industrial group of enzymes in family 13 glycosyl hydrolases. They hydrolyze either α-1,6 and α-1,4 or both glycosidic bonds in pullulan as well as other carbohydrates to produce glucose, maltose, and maltotriose syrups, which have important uses in food and other related sectors. However, very less reports are available on pullulanase production from native strains because of low yield issues. In line with the increasing demands for pullulanase, it has become important to search for novel pullulanase-producing microorganisms with high yields. Moreover, high production costs and low yield are major limitations in the industrial production of pullulanase enzymes. The production cost of pullulanase by using the solid-state fermentation (SSF) process can be minimized by selecting agro-industrial waste. This review summarizes the types, sources, production strategies, and potential applications of pullulanase in different food and other related industries. Researchers should focus on fungal strains producing pullulanase for better yield and low production costs by using agro-waste. It will prove a better enzyme in different food processing industries and will surely reduce the cost of products.

show abstract

Effect of substrate and culture conditions on the production of amylase and pullulanase by thermophilic Clostridium thermosulforegenes SVM17 in solid state fermentation

Cited by 7 publications

References 43 publications

Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium

Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium

Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation

Pullulanase: unleashing the power of enzyme with a promising future in the food industry

Contact Info

Product

Resources

About