Effect of Sucrose and Boric Acid on in-vitro Pollen Germination of Guava (Psidium guajava) Varieties

Sarkar, Tanmoy; Sarkar, Shrabana; Vangaru, Sathish

doi:10.9734/air/2018/41145

Cited by 13 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the media, for example, those of Prunus laurocerasus (Sulusoglu and Cavusoglu, 2014), Garcinia mangostana (Sutthinon et al, 2018), Triticum aestivum (Turan et al, 2018), and Hydrangea macrophylla, Dichroa febrifuga and their hybrids (Alexander, 2019) have been developed based on the Brewbaker and Kwack medium, which comprises 10% sucrose, 100 ppm boric acid, 300 ppm calcium nitrate, 200 ppm magnesium sulfate, and 100 ppm potassium nitrate (Brewbaker and Kwack, 1963). In fact, in the most essential ones, sucrose or/and boric acid is sufficient; (Sarkar et al, 2018); however, the presence of iron will improve germination or extension (Brewbaker and Kwack, 1963). An optimal medium for pitaya pollen germination is essential for an in vitro pollen study.…”

Section: Introductionmentioning

confidence: 99%

Pollen germination and hand pollination in pitaya (Selenicereus spp.)

Shi

Dai

et al. 2022

Emir J Food Agric

View full text Add to dashboard Cite

Hand pollination is a necessary assisting method for pitaya (Selenicereus spp.) production to achieve a high yield. With the cultivated area increasing at an exponential rate in recent years, a comprehensive study of the pollination process was conducted. We developed an ideal medium for pitaya pollen in vitro germination in this study, then tested the activity of pollen collected from or stored for various time periods. We discovered that those collected between 2 h before blooming and 6 h after blooming had the higher germination rates (27.2–65.1%), the highest activity was at 2 h after blooming, and that storing them at 4°C for 24 hours reduces their germination rate from 65.2 percent to 35.5 percent and their production to about 82%. As a result, pollinating plants with pollen that has been held for more than 24 hours is not recommended unless a breakthrough in pollen storage is achieved. We also discovered that stigma receptivity and pollen activity are synchronized, which together determines the rate of fruit setting and the size of the fruit. Pollination within 6 hours after flowering offers the optimum fruit setting percentage and size, while pollination at 6:00 pm, 2 hours before blooming, is also a good alternative; however, pollination at 6:00 am the next morning is expected to result in a 23 % drop in productivity. These findings will be beneficial for reproductive biology research, as well as laying the groundwork for hand pollination to boost pitaya output and breeding efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

Pollen germination and hand pollination in pitaya (Selenicereus spp.)

Shi

Dai

et al. 2022

Emir J Food Agric

View full text Add to dashboard Cite

show abstract

“…The sucrose concentration is an important factor due to promote osmotic balance between pollen and culture media and acts as a source of energy (Sarkar;Sarkar;Vangaru, 2018). Abiotic factors related to incubation conditions, as temperature and presence or absence of light can be considered for the pollen studies.…”

Section: Introductionmentioning

confidence: 99%

In vitro viability of pollen grain of fiji dwarf coconut accession

Machado

Vendrame

Neto

et al. 2021

PCC&M

View full text Add to dashboard Cite

Lethal yellowing is a disease that causes severe damages to coconut palms and it has devastated the coconut canopy of several Caribbean islands, and areas of southern Florida. The Fiji dwarf coconut accession has demonstrated tolerance to this disease. Pollen storage and germination provides useful information for conservation strategy and genetic improvement programs. The objective of this study was to evaluate pollen viability of Fiji dwarf coconut by in vitro germination under different nutritional and abiotic conditions. For the first experiment, two liquid culture media were evaluated for in vitro germination of Fiji dwarf coconut pollen; the Lora culture medium and the Armendariz culture medium combined with four temperature levels. In the second experiment, different sucrose concentrations in the Lora culture medium (4, 8, 12 and 16%) were evaluated combined with two incubation times (6 and 24 h) in the presence or absence of light. The highest in vitro germination of pollen grain was obtained in Lora culture medium by 24 h incubation period at 28 ºC. The light condition does not influence the germination process. In vitro germination percentage improved as the sucrose concentration increased according to a linear regression. In contrast, damage pollen grain percentage reduced according to a quadratic regression.

show abstract

“…Guava is a cross-pollinated crop but self-pollination also occurred [8,9]. In guava, studies showed that the distribution of cross-pollination by insects is about 35% and it is said that cross-pollination was the most common form of pollination in guava [10].…”

Section: Introductionmentioning

confidence: 99%

Pollination in the Tropics: Role of Pollinator in Guava Production

Omar

ZARİMAN

Abdul

2021

International Journal of Life Sciences and Biotechnology

View full text Add to dashboard Cite

Pollinators are the key point for successful pollination in most flowering plants where around 90% of plants depend entirely on them. Majority of the pollinating species are wild, comprising bees, certain species of flies, wasps, moths, butterflies, thrips, beetles, bats, birds and other vertebrates, and among them insect pollinators are the most crucial. Fruit crops benefit in an impressive way from insect pollination, where there is a remarkable improvement both in the productivity and the quality of self-fertilized, self-incompatible and cross-cropping crops worldwide. The pollinators are responsible for assisting these flowering plants with their reproduction. However, the crisis of the pollinator's decline (wild and managed pollinators) which could seriously disrupt pollination activities in the ecosystems has attracted the attention of the world. Despite the growing concern about the decline in pollinators worldwide, some issues remain uncertain as data are often limited and undermined. Guava, Psidium guajava is a marketable fruit in numerous tropical and subtropical regions around the world. There has been a growing interest in pollination studies on guava because of its great economic importance. In guava, self-pollination is evident, however, it benefits greatly from insect pollination. This article aims to provide an overview of tropical pollination and pollination problems that have occurred around the world with a focus on pollination activities in guava.

show abstract

Effect of Sucrose and Boric Acid on in-vitro Pollen Germination of Guava (Psidium guajava) Varieties

Cited by 13 publications

References 16 publications

Pollen germination and hand pollination in pitaya (Selenicereus spp.)

Pollen germination and hand pollination in pitaya (Selenicereus spp.)

In vitro viability of pollen grain of fiji dwarf coconut accession

Pollination in the Tropics: Role of Pollinator in Guava Production

Contact Info

Product

Resources

About