Effect of Surface Roughness and Composition on Costochondral Chondrocytes is Dependent on Cell Maturation State

Boyan, Barbara D.; Lincks, J.; Lohmann, Christoph H.; Sylvia, V. L.; Cochran, David L.; Blanchard, Cheryl R.; Dean, David D.; Schwartz, Zvi

doi:10.2106/00004623-199911000-00026

Cited by 11 publications

(14 citation statements)

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“…Of the eight genes related to bone and cartilage differentiation that we analyzed, two bone‐related genes and two cartilage‐related genes were expressed at a higher level at 24 h on the Ra (5.8 μm) surface than on the Ra (0.14 μm) surface and none were lower on the Ra (5.8 μm) surface, indicating that at this early time point the Ra (5.8 μm) surface provided a stronger stimulus for maturation to these two lineages than the Ra (0.14 μm) surface. Cells of both the osteogenic and chondrogenic lineage have been previously shown to be affected by surface roughness in culture 19, 29–31. Therefore, it is not surprising that we have seen genes associated with both lineages up‐regulated on the Ra (5.8 μm) titanium surface in our cultures.…”

Section: Discussionmentioning

confidence: 52%

Patterns of gene expression in rat bone marrow stromal cells cultured on titanium alloy discs of different roughness

Leven

Virdi²,

Sumner³

2004

J Biomedical Materials Res

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Rat bone marrow stromal cells were cultured on either Ra (0.14 microm) or Ra (5.8 microm) Ti6Al4V discs for 24 or 48 h. Cells on the Ra (0.14 microm) surface showed typical fibroblastic morphology, whereas cells on the Ra (5.8 microm) surface were in clusters with a more epithelial appearance. RNA was extracted from the cells at both time points, and gene expression was analyzed by using a rat gene microarray. At 24 and 48 h, a similar number of genes were both up- and down-regulated at least twofold on the Ra (5.8 microm) surface compared to the Ra (0.14 microm) surface. We analyzed the relative level of specific groups of genes related to bone and cartilage development, cell adhesion and extracellular matrix proteins, transcription factors, bone morphogenetic proteins, phospholipases, and protein kinases. Roughness did not appear to be a specific stimulator of osteogenesis because genes of both the bone and cartilage lineage were up-regulated on the Ra (5.8 microm) surface. The most prominent change among transcription factors was up-regulation of Hox 1.4 on the Ra (5.8 microm) surface. Up-regulation of phospholipase A2 and SMAD 4 indicate these genes are also involved in the response of cells to an Ra (5.8 microm) surface. Our data show surface roughness alters the expression of a large number of genes in marrow stromal cells, which are related to multiple pathways of mesenchymal cell differentiation.

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Section: Discussionmentioning

confidence: 52%

Patterns of gene expression in rat bone marrow stromal cells cultured on titanium alloy discs of different roughness

Leven

Virdi²,

Sumner³

2004

J Biomedical Materials Res

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“…While CHT particulates preserve their spherical morphology, elongated filiform structures can be also noticed in the blended regions of the film (S2-S4). One can assume that these thread-like structures may support some toughness improvements similar to the one provided by fiber reinforcing in the biocomposite materials, ensuring the desired mechanical behavior for tissue substitution [76].…”

Section: Case Study: Chitosan-based Biocompositesmentioning

confidence: 99%

Composite Coatings Based on Renewable Resources Synthesized by Advanced Laser Techniques

Visan¹,

Ristoscu²,

Mihăilescu³

2016

Composites From Renewable and Sustainable Materials

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This chapter reviews the progress and perspectives of composite materials in the form of thin films based on renewable resources for biofabrication of a new generation of medical implants with antibacterial properties. The chapter starts with an overview of the types of renewable materials that were currently studied and of the unique properties which make them perfect candidates for numerous bio-related applications. A briefing of recent progresses in the field of advanced laser synthesis of composites from renewable and sustainable materials, as well as the relevant results in our researches is made. The discussion spans composite coatings based on renewable resources, [e.g., chitosan (CHT) and lignin (Lig)] as biomaterials intended for metallic implants. A particular attention is given to lignin synthesis in the form of thin films due to its ability to functionalize the medical implant surface while preserving the similar composition and the structural properties of the raw, renewable biomaterial. We focused on recent technological advancements (e.g., matrix-assisted pulsed laser evaporation (MAPLE) and Combinatorial-MAPLE) which have brought the spotlight onto renewable biomaterials, by detailing the relevant engineering data of processing. This chapter concludes that the extensions of advanced laser techniques are viable fabrication methods of a new generation of metallic implants.

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“…It has been observed that chondrocytes are affected by surface roughness and that micrometrically grooved topographies can alter cell adhesion, cytoskeleton, and migration of chondrocytes depending on their cell passage. 8,12,13 Moreover, the aggregation of primary chondrocytes is stimulated when cultured on microgrooved substrates, and hyaluronan micropatterned surfaces preserved the chondrogenic phenotype compared with polyethylene teraphthalate surfaces. 14 These studies demonstrated alteration in mature chondrocyte behavior owing to biomechanical cues.…”

Section: Introductionmentioning

confidence: 99%

Topography-Guided Proliferation: Distinct Surface Microtopography Increases Proliferation of ChondrocytesIn Vitro

Joergensen

Andersen

et al. 2015

Tissue Engineering Part A

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Chondrocyte-based cartilage repair techniques require control of articular chondrocyte expansion ex vivo. Articular chondrocytes have limited availability, and prolonged culturing to obtain a cell number sufficient for clinical use often results in phenotypic alterations and increased costs. In this study, we applied a screening library consisting of micrometer-sized topographical features, termed biosurface structure array (BSSA), to identify specific topographical microstructures affecting the proliferation of human chondrocytes in passage 1 (P1) or 2 (P2). The BSSA library comprised 10 patterns and 16 combinations of pillar size (X) and interpillar gap size (Y). Specific microstructures significantly increased the chondrocytes' proliferative responsiveness in term of patterns, X and Y for P2 compared with P1. The P1 and P2 chondrocytes responded independently to similar patterns after 4 days of culturing, whereas only chondrocytes at P2 responded to specific microstructures with Y = 1 μm and X = 2, 4 μm by a 2.3- and 4.4-fold increased proliferation, respectively. In conclusion, these findings indicate that specific surface topographies promote chondrocyte proliferation and may, indeed, be a tool to control the behavior of chondrocytes in vitro.

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Effect of Surface Roughness and Composition on Costochondral Chondrocytes is Dependent on Cell Maturation State

Cited by 11 publications

References 0 publications

Patterns of gene expression in rat bone marrow stromal cells cultured on titanium alloy discs of different roughness

Patterns of gene expression in rat bone marrow stromal cells cultured on titanium alloy discs of different roughness

Composite Coatings Based on Renewable Resources Synthesized by Advanced Laser Techniques

Topography-Guided Proliferation: Distinct Surface Microtopography Increases Proliferation of ChondrocytesIn Vitro

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