Patterns of gene expression in rat bone marrow stromal cells cultured on titanium alloy discs of different roughness

Leven, Robert M.; Virdi, Amarjit S.; Sumner, Dale R.

doi:10.1002/jbm.a.30082

Cited by 29 publications

(23 citation statements)

References 37 publications

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“…The powder-based 3D printing process enabled us to create structures with a high surface roughness. In literature, the influence of surface roughness on cell attachment and proliferation is described mainly in conjunction with grinded surfaces and is discussed controversial [17]. When trying to relieve cells from our HA-S with Trypsin in preliminary studies, we observed very strong cell attachment.…”

Section: Discussionmentioning

confidence: 93%

Biocompatibility of ceramic scaffolds for bone replacement made by 3D printing

Leukers¹,

Gülkan²,

Irsen

et al. 2005

Mat.-wiss. u. Werkstofftech.

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Bone replacement materials used in tissue engineering require a high degree of safety and biological compatibility. For these reasons synthetic bone replacement materials based on calcium-phosphates are being used more widely. To mimic natural bone, rapid prototyping processes and especially 3D printing are favourable. Using 3D printing, complex 3 dimensional structures can be made easily.In this study we successfully performed biocompatibility tests with a Hydroxyapatite test structure (HA-S) made by 3D printing. Cytotoxicity tests were carried out according to DIN ISO 10993-5 in static and dynamic cultivation setups. To estimate cell proliferation and analyze morphology, histological evaluation was done. In summary, good cell viability as well as good proliferation behaviour were found. Moreover, these results show that the 3D printing process in combination with the suitable material presented in this study is well suited for fabricating scaffolds for TE in the required accuracy and biological compatibility.Key words: Scaffold fabrication, cell culture, biocompatibility, hydroxyapatite, 3D printing Knochenersatzmaterialien für das Tissue Engineering (TE) erfordern ein hohes Maß an Sicherheit und biologischer Verträglichkeit. Aus diesen Gründen nehmen synthetische Knochenersatzmaterialien auf der Basis von Calciumphosphaten einen immer größer werdenden Stellenwert ein. Um der Struktur des natürlichen Knochens so nahe wie möglich zu kommen, bieten sich Verfahren aus dem Rapid Prototyping, insbesondere das 3D Drucken an. Dadurch lassen sich komplexe, dreidimensionale Strukturen für das TE einfach herstellen.In dieser Studie haben wir erfolgreich eine mittels 3D Drucken hergestellte Struktur (HA-S) auf der Basis von Hydroxylapatit auf ihre Biokompatibilität getestet. Zytotoxizitätsuntersuchungen wurden analog DIN ISO 10993-5 in statischer und dynamischer Kultivierung durchgeführt. Zudem wurden histologische Schnitte zur Beurteilung der Zellproliferation und -morphologie durchgeführt. Insgesamt konnte eine gute Zellvitalität und Zellproliferation nachgewiesen werden. Darüber hinaus zeigen die Ergebnisse, dass das hier vorgestellte 3D Druckverfahren mit dem hier verwendeten Material dazu geeignet ist, Strukturen für das TE in ausreichender Prä-zision und biologischer Verträglichkeit herzustellen.

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Section: Discussionmentioning

confidence: 93%

Biocompatibility of ceramic scaffolds for bone replacement made by 3D printing

Leukers¹,

Gülkan²,

Irsen

et al. 2005

Mat.-wiss. u. Werkstofftech.

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“…39 In addition, they produce higher levels of growth factors and cytokines that regulate osteogenesis including PGE2, which is required for their differentiation, 40,41 and osteoprotegerin, 42 which can modulate osteoclastic bone resorption. 43 Similarly, osteoblastic differentiation of bone marrow stromal cells and fetal rat calvarial cells is also surface dependent, [44][45][46] but the role of surface properties on differentiation of isolated human MSCs was not previously examined. While we did not address this question directly, our results suggest that surface-dependent changes in MSCs make them more sensitive to BMP-2, and the BMP-2-treated cells are more responsive to PEMF.…”

Section: Discussionmentioning

confidence: 99%

Pulsed electromagnetic fields enhance BMP‐2 dependent osteoblastic differentiation of human mesenchymal stem cells

Schwartz

Simon

Durán

et al. 2008

Journal Orthopaedic Research

157

126

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Mesenchymal stem cells (MSCs) express an osteoblastic phenotype when treated with BMP-2, and BMP-2 is used clinically to induce bone formation although high doses are required. Pulsed electromagnetic fields (PEMF) also promote osteogenesis in vivo, in part through direct action on osteoblasts. We tested the hypothesis that PEMF enhances osteogenesis of MSCs in the presence of an inductive stimulus like BMP-2. Confluent cultures of human MSCs were grown on calcium phosphate disks and were treated with osteogenic media (OM), OM containing 40 ng/mL rhBMP-2, OM þ PEMF (8 h/day), or OM þ BMP-2 þ PEMF. MSCs demonstrated minor increases in alkaline phosphatase (ALP) during 24 days in culture and no change in osteocalcin. OM increased ALP and osteocalcin by day 6, but PEMF had no additional effect at any time. BMP-2 was stimulatory over OM, and PEMF þ BMP-2 synergistically increased ALP and osteocalcin. PEMF also enhanced the effects of BMP-2 on PGE2, latent and active TGF-b1, and osteoprotegerin. Effects of PEMF on BMP-2-treated cells were greatest at days 12 to 20. These results demonstrate that PEMF enhances osteogenic effects of BMP-2 on MSCs cultured on calcium phosphate substrates, suggesting that PEMF will improve MSC response to BMP-2 in vivo in a bone environment. ß

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“…on day 7 after fracture, GFP-and von Kossa-positive cells were observed around the titanium screws of the fixation plate. Several studies have reported that titanium stimulates osteoblastic differentiation in mesenchymal and osteoblastic cells [10,12,13,26]. For example, Wall et al [26] reported that titanium surfaces accelerate osteogenic differentiation of mesenchymal stromal cells in vitro.…”

Section: Discussionmentioning

confidence: 99%