Effect of surface-treatments on flexibility and guided bone regeneration of titanium barrier membrane

Abstract

Titanium barrier membranes are prepared to investigate the effect of surface-treatments, such as machining, electropolishing, anodizing, and electropolishing + TiN coating, on the biocompatibility and physical properties of the membranes. The surface roughness (Ra) of the membrane decreases from machining (0.37 ± 0.09 µm), TiN coating (0.22 ± 0.09 µm), electropolishing (0.20 ± 0.03 µm), to anodizing (0.15 ± 0.03 µm). The highest ductility (24.50 %) is observed for the electropolished Ti membrane. No evidence of causing cell lysis or toxicity is found for the membranes regardless of the surface-treatments. Cell adhesion results of L-929 and MG-63 show that the machined Ti membrane exhibits the highest cell adhesion while the electropolished membrane is the best membrane for the L-929 cell proliferation after 7 days. However, no appreciable difference in MG-63 cell proliferation among variously surface-treated membranes is detected, suggesting that the electropolished Ti membrane is likely to be the best membrane due to the synergic combination of tailored flexibility and excellent fibroblast proliferation.

Mechanical properties and cytotoxicity of PLA/PCL films

“…In this study, the 97/3 PLA/PCL scaffolds with TBC was considered as the material of choice due to the tailored strength and ductility, which was much better than mechanical properties of pure PLA and PCL films. A cytotoxicity test of the PLA/PCL films determines whether a product or compound will have a toxic effect on living cells [7,8,[10][11][12][13]. The test extracts from the PLA/ PCL films showed no evidence of causing cell lysis or toxicity regardless of TBC presence, as shown in Fig.…”

“…The extract test method was conducted on the PLA/PCL films to evaluate the potential of cytotoxicity on the base of the International Organization for Standardization (ISO 10993-5) [6][7][8][10][11][12][13]. The PLA/PCL films were extracted aseptically in single strength Minimum Essential Medium [1X MEM, Dulbecco's Modified Eagles's Medium (Gibco) with 10% fetal bovine serum (Gibco) and 1% penicillinstreptomycin] with serum.…”