Effect of surfactants and temperature on germination and vegetative growth of Beauveria bassiana

Abstract

Three non-ionic surfactants: Tween20, Tween80 and Breakthru ® were screened for their effects on spore germination and mycelial growth rates and for their influence on three isolates of Beauveria bassiana spore germination at various temperatures. Tween20 and Tween80 were compatible with all the B. bassiana isolates in the germination studies, but inhibited germination at higher surfactant concentrations, irrespective of the conidial concentrations . Breakthru ® had an inhibitory effect on germination even at the lowest concentration of 0.1% on all the B. bassiana isolates. The effects of the surfactants on spore germination did not correspond with their effects on colony growth. Conidial viability within the same formulation declined significantly with increases in temperature, irrespective of the surfactant. The optimal temperature for conidial germination of B. bassiana isolates was approximately 25 °C with an upper limit at 30 °C. Isolate 7320 was identified as the least affected by the different surfactants. This isolate was able to germinate rapidly in a broad temperature range of 25–30 °C after 24 h, this characteristic being an essential factor in controlling house fly populations in poultry houses.

Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

“…Accordingly, in P. chrysogenum metabolic changes are observed as stress result of adding detergents to liquid broths, with consequent production of different organic acids (Jakovljevi et al, 2014) Finally, Tween 40 and 80 did not enhance eritadenine yields, but biomass production did significantly. Similar growth stimulation by Tween 80 in submerged cultures have been observed in Beauveria (Mwamburi et al, 2015), and Armillaria sp. (Hadibarata and Kristanti, 2013).…”

Remediation of Artificially Hydrocarbon Polluted Vadose Zone Soil in Glass Column through Percolation with Solution of Nutrient, Nutrient-Surfactant or Surfactant

“…Selected surfactants have been shown by Chen et al, (2006) to restrain the degradation of polycyclic aromatic hydrocarbons by white-rot fungi. In other related studies, surfactants have been shown to inhibit spore germination and ergosterol production by fungi (Kozirog et al, 2018;Mwamburi et al, 2015;Tanuja et al, 2010). It can thus be generalized that surfactants puts a pressure on the hydrocarbon degrading ability of fungi, and this pressure can be relaxed in the presence of nutrients as observed in this study.…”

The virulence improvement of Beauveria bassiana in infecting Cylas formicarius modulated by various chitin based compounds

“…Moreover, the level of moisture surrounding the insect is a key factor in forming germ tubes after the adhesion of conidia to the insect (Gaborty et al 2014;Devi and Bai 2015). The suitable moisture for conidia germination at the penetration and infection step should be above 95% (Mwanburi et al 2015). Fungi penetrate into the insect cuticle by producing various chitinase and proteases enzymes to degrade the coat compounds (Ortiz-urquiza and Keyhani, 2013).…”