Effect of sweetener containing Stevia on the development of dental caries in enamel and dentin under a microcosm biofilm model

Nascimento, Caren Augustinho do; Kim, Rafaela Ricci; Ferrari, Carolina Ruis; Souza, Beatriz Martines de; Braga, Aline Silva; Magalhães, Ana Carolina

doi:10.1016/j.jdent.2021.103835

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…It is pertinent to highlight a study conducted by Augustinho et al, which observed that some commercial formulations of stevia and aspartame exhibited cariogenic potential similar to that of sucrose, possibly due to the presence of lactose as an impurity in them. Conversely, the study revealed that the pure forms of these artificial sweeteners did not demonstrate cariogenic properties [46]. This suggests that the effectiveness of stevia may depend on its concentration (or purity) in addition to various other factors, such as the type of bacterial species and strains, quorum sensing, and signaling pathways that regulate biofilm formation and EPS production, as well as the presence of other dietary carbohydrates such as sucrose.…”

Section: Discussionmentioning

confidence: 96%

Sugar Substitute Stevia Inhibits Biofilm Formation, Exopolysaccharide Production, and Downregulates the Expression of Streptococcal Genes Involved in Exopolysaccharide Synthesis

AlKanderi,

AlFreeh,

Bhardwaj

et al. 2023

Dentistry Journal

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Background: Acid production by sucrose fermentation disturbs the balance in dental plaque by lowering the oral pH. As a consequence of the profound effect of sucrose on caries initiation and progression, many studies have been directed towards finding non-cariogenic artificial sweeteners that can be used as a substitute to sucrose. Existing literature shows that dietary sucrose upregulates the expression of biofilm associated genes involved in exopolysaccharide (EPS) production. Objective: In this study, we aimed to investigate the effect of the sugar substitute stevia on biofilm formation, EPS secretion, and streptococcal genes encoding glucan-binding proteins (Gbps) and glucosyltransferases (Gtfs), which are essential for the synthesis of EPS. Materials and Methods: Streptococcus mutans and Streptococcus gordonii were grown as biofilm cultures with or without stevia and sucrose. Biomass was quantified for biofilm and EPS production by crystal violet staining and the phenol–sulfuric acid method, respectively. Expression of gtfB and gbpB genes was studied by RT-PCR. Results: The quantities of biofilm were significantly lower when grown in the presence of stevia compared to sucrose in both species (p < 0.05). The proportion of EPS in the biofilm pellet decreased with increasing concentrations of stevia in both species but remained nearly unchanged with sucrose with respect to the control. In both streptococcal species, exposure of stevia decreased the expression of gtfB and gbpB genes compared to sucrose (p < 0.05). In comparison to the untreated control, the expression was decreased in the presence of stevia in both species, while it increased 2.5- to 4-fold in S. mutans and 1.5- to 2.5-fold in S. gordonii in the presence of sucrose. Conclusion: The ability of stevia to inhibit biofilm formation, reduce EPS production, and downregulate the expression of gtfB and gbpB genes in S. mutans and S. gordonii may have potential therapeutic applications in controlling dental plaques and caries.

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Section: Discussionmentioning

confidence: 96%

Sugar Substitute Stevia Inhibits Biofilm Formation, Exopolysaccharide Production, and Downregulates the Expression of Streptococcal Genes Involved in Exopolysaccharide Synthesis

AlKanderi,

AlFreeh,

Bhardwaj

et al. 2023

Dentistry Journal

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“…Parameter yang dapat digunakan untuk mengukur kesukaan responden terhadap nutrasetika teh herbal yaitu warna, bau dan rasa. Pengujian ini untuk mendapatkan formula optimal dengan variasi kandungan Stevia sebagai perasa (do Nascimento, 2021;Tehranian, 2023). Dari hasil uji tingkat kesukaan melalui tanggapan rasa menunjukkan bahwa formulasi kedua merupakan formulasi yang paling diterima oleh responden dengan hasil total skor tertinggi.…”

Section: Pembahasanunclassified

Formulasi Sediaan Nutrasetikal Teh Celup Kombinasi Bunga Rosella (Hibiscus sabdariffa) dan Bunga Kenop (Globe amaranth) dengan Variasi Konsentrasi Stevia sebagai Perasa

Agustina,

Amaranggana,

Yuliati

et al. 2024

phms

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Telah dilakukan penelitian formulasi nutrasetikal sediaan teh celup kombinasi bunga rosella (Hibiscus sabdarifa) dan bunga kenop (Globe amaranth) dengan variasi konsentrasi stevia sebagai perasa. Stevia dalam formulasi berfungsi sebagai pengganti gula dan memiliki aktivitas antioksidan. Penelitian ini bertujuan mengevaluasi pengaruh konsentrasi stevia pada kesukaan responden. Formula teh celup terdiri atas 0,7% bunga Rosella (Hibiscus sabdarifa) dan 1% bunga kenop (Globe amaranth) dengan variasi konsentrasi konsentrasi stevia yaitu 1,3% (Formula 1), 1% (Formula 2) dan 0,7% (Formula 3). Ekstrak bunga rosella (Hibiscus sabdarifa) dan bunga kenop (Globe amaranth) dievaluasi kandungan metabolit sekundernya. Formula teh celup dievaluasi dengan uji organoleptis, uji kadar air dan kesukaan responden. Hasil skrining fitokimia menunjukkan ekstrak bunga Rosela dan bunga Kenop mengandung flavonoid yang berfungsi sebagai antioksidan. Berdasarkan hasil penelitian menunjukkan bahwa bunga Rosella dan bunga Kenop dapat digunakan dalam formulasi nutrasetikal teh celup. Evaluasi organoleptis dari ketiga formula menunjukkan karakteristik bau khas teh, warna merah kecoklatan dan kadar air kurang dari 10% (sesuai dengan persyaratan dalam Materia Medika Indonesia). Hasil kesukaan pada responden menunjukkan bahwa warna, rasa, dan bau khas sediaan teh celup herbal dapat diterima. Dari hasil uji kesukaan juga menunjukkan bahwa formula kedua adalah yang paling banyak disukai oleh responden.

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“…With the development of the food industry, sugar-substituted drinks have gained popularity among the public in recent years. They provide the sweetness of sugars without increasing calories, thereby reducing the risk of obesity and dental caries [ 11 , 12 ]. However, they may have adverse effects on children’s health.…”

Section: Introductionmentioning

confidence: 99%

Impact of Replacing Soft Drinks with Dairy Products on Micronutrient Intakes of Chinese Preschool Children: A Simulation Study

Zhuang,

Yin,

Han

et al. 2023

Nutrients

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At present, energy surplus and micronutrient deficiency coexist in preschool children in China. The low intake of dairy products accompanied by an increased consumption of soft drinks in this age group reveals some of the reasons for this phenomenon. The purpose of this study was to evaluate the improvement of key micronutrients in preschool children by quantifying the dietary nutritional gap before and after simulating the use of dairy products instead of equal amounts of soft drinks. In the cross-sectional dietary intake survey of infants and young children in China (2018–2019), 676 preschool children aged 3–6 years were randomly selected. Four days of dietary data were collected through an online diary for simulation. The individual intake of soft drinks was substituted at a corresponding volume by soymilk, cow’s milk, or formulated milk powder for preschool children (FMP-PSC). In these three models, the simulated nutrient intake and nutrient inadequacy or surplus were compared with the actual baseline data of the survey. The results of this study indicated that all three models made the nutrient intakes of this group more in line with the recommendations. For the whole population, the replacement of soymilk improved the intake of zinc (from 4.80 to 4.85 mg/d), potassium (from 824.26 to 836.82 mg/d), vitamin A (from 211.57 to 213.92 μg retinol activity equivalent/d), and vitamin B9 (from 115.94 to 122.79 μg dietary folate equivalent/d); the simulation of cow’s milk improved the intake of calcium (from 311.82 to 330.85 mg/d), zinc (from 4.80 to 4.87 mg/d), potassium (from 824.26 to 833.62 mg/d), vitamin A (from 211.57 to 215.12 μg retinol activity equivalent/d), vitamin B2 (from 0.53 to 0.54 mg/d), and vitamin B12 (from 1.63 to 1.67 μg/d); and the substitution of FMP-PSC improved the intake of calcium (from 311.82 to 332.32 mg/d), iron (from 9.91 to 9.36 mg/d), zinc (from 4.80 to 4.96 mg/d), potassium (from 824.26 to 828.71 mg/d), vitamin A (from 211.57 to 217.93 μg retinol activity equivalent/d), vitamin B2 (from 0.53 to 0.54 mg/d), vitamin B9 (from 115.94 to 118.80 μg RA dietary folate equivalent/d), and vitamin B12 (from 1.63 to 1.70 μg/d). Therefore, correct nutritional information should be provided to parents and preschool children. In addition to changing the consumption behavior of soft drinks, it is also necessary to have a diversified and balanced diet. When necessary, the use of food ingredients or nutritional fortifiers can be encouraged.

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Effect of sweetener containing Stevia on the development of dental caries in enamel and dentin under a microcosm biofilm model

Cited by 5 publications

References 39 publications

Sugar Substitute Stevia Inhibits Biofilm Formation, Exopolysaccharide Production, and Downregulates the Expression of Streptococcal Genes Involved in Exopolysaccharide Synthesis

Sugar Substitute Stevia Inhibits Biofilm Formation, Exopolysaccharide Production, and Downregulates the Expression of Streptococcal Genes Involved in Exopolysaccharide Synthesis

Formulasi Sediaan Nutrasetikal Teh Celup Kombinasi Bunga Rosella (Hibiscus sabdariffa) dan Bunga Kenop (Globe amaranth) dengan Variasi Konsentrasi Stevia sebagai Perasa

Impact of Replacing Soft Drinks with Dairy Products on Micronutrient Intakes of Chinese Preschool Children: A Simulation Study

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