Effect of tamoxifen treatment on global and insulin-like growth factor 2-H19 locus-specific DNA methylation in rat spermatozoa and its association with embryo loss

Semen samples showing an increased rate of spermatozoa with imprinting errors have a negligible effect in the outcome of assisted reproduction techniques

“…DNA methylation is subject to alterations that could be caused by genetic factors (variations in the DNA sequence of factors involved in the methylation establishment or maintenance), [5][6][7] intrauterine environment, 8 toxin exposure, 9 hormone treatment 10,11 and diet. 12 Some authors have related the presence of imprinting errors in newborns to the application of assisted reproduction techniques (ART).…”

Effects of Aroclor 1254 on the Dna Methylation of Imprinted Genes in the Adult Mouse Sperm

“…ER over-expression and DNA methylation modification of imprinted genes regulate the relative gene expression . Reduced DNA methylation at imprinted loci in rat spermatozoa upon tamoxifen treatment indicated a role of estrogen-associated signaling in the acquisition of paternal-specific imprints during spermatogenesis (Pathak et al, 2009). The Aroclor 1254 exposure led to a significant reduction in the methylation patterns at the differentially methylated region of paternally imprinted genes H19 and Gtl2 and a significant increase in the methylation patterns of maternally inherited genes Mest, Snrpn, and Igf2r.…”

“…Recent study has shown that in utero exposure of male rats with an antiandrogenic compound, vinclozolin, results in epigenetic transgenerational disease phenotype involving alterations in DNA methylation patterns at various loci in the spermatozoa (Chang et al, 2006). Reduced DNA methylation at imprinted loci in rat spermatozoa upon tamoxifen treatment indicated a role of estrogen-associated signaling in the acquisition of paternal-specific imprints during spermatogenesis (Pathak et al, 2009). In addition, association between DNA methylation and postimplantation loss suggests that errors in paternal imprints at imprinted loci could affect embryo development (Pathak et al, 2009).…”

“…Reduced DNA methylation at imprinted loci in rat spermatozoa upon tamoxifen treatment indicated a role of estrogen-associated signaling in the acquisition of paternal-specific imprints during spermatogenesis (Pathak et al, 2009). In addition, association between DNA methylation and postimplantation loss suggests that errors in paternal imprints at imprinted loci could affect embryo development (Pathak et al, 2009). These experiments indicate that normal methylation patterns of imprinted genes in the male germ line are crucial for normal spermatogenesis and embryogenesis.…”

Association of progesterone receptor gene polymorphism with male infertility and clinical outcome of ICSI

“…It is possible that defects in the paternal genome and/or abnormal RNA profiles in the spermatozoa may lead to failure of embryonic growth, pregnancy loss, and/or developmental abnormalities. Recent clinical and experimental evidences have indeed suggested that epigenetic defects in the sperm DNA is associated with recurrent spontaneous abortions [4,31]. Since the PR mRNA is expressed in the mature spermatozoa [37,38] and the PROGINS polymorphism is associated with defective PR transcription [11,33], we tested if the PROGINS insertion in the paternal genome has any effect on fertilization, embryo quality and pregnancy rates.…”