Effect of tea polyphenols on histamine release from rat basophilic leukemia (RBL‐2H3) cells: the structure–inhibitory activity relationship

Abstract

We studied the effect of tea polyphenols on histamine release from rat basophilic leukemia (RBL-2H3) cells. Among tea polyphenols, (-)- epigallocatechin gallate (EGCG) most strongly and dose-dependently inhibited histamine release from cells stimulated with a calcium ionophore, A23187. (-)-Epigallocatechin (EGC) and (-)-epicatechin gallate (ECG) with a triphenol residue moderately inhibited histamine release, whereas diphenolic (+)-catechin (C) and (-)-epicatechin (EC) did not. The magnitude of the inhibitory effect was in the order EGCG > ECG > EGC. Among simple polyphenols, the triphenol compounds, pyrogallol (PG) and gallic acid (GA) exerted inhibitory activity, but the diphenols, pyrocatechol, hydroquinone, and resorcinol did not. In addition, the mixture of PG and GA inhibited histamine release as strongly as EGCG with two triphenol residues. Similarly, they inhibited histamine release induced by IgE-antigen complex stimulation more efficiently than that induced by A23187 stimulation. EGCG did not inhibit the increase of intracellular Ca2+ in RBL-2H3 cells stimulated with A23187 or IgE antigen. These results indicate that the triphenol structure plays an important role in the inhibitory activity of tea polyphenols. Their activity seemed to be exerted through the metabolic events occurring after the elevation of intracellular Ca2+ concentration.

Isolation of a New Anti-Allergic Phlorotannin, Phlorofucofuroeckol-B, from an Edible Brown Alga,Eisenia arborea

“…RBL-2H3 14) cells precultured for 3 d in Eagle's modified essential medium supplemented with 20% fetal bovine serum were sensitized with anti-dinitrophenyl (DNP) IgE for 18 h. The medium was then changed to Tyrode buffer (pH 7.2), 15) and the solution of the test sample was added. After incubation for 10 min, the cells were treated with an antigen, DNP-BSA, for 35 min.…”

Inhibitory effect of tannins from galls of Carpinus tschonoskii on the degranulation of RBL-2H3 Cells

“…In vivo anti-inflammatory and antioxidant properties of ET metabolite Urolithin A and Urolithin B were also reported (Larrosa et al 2010;Ishimoto et al 2011). Furthermore, ETs such as geraniin is hydrolyzed to GA by microflora (Ito 2011), also gallotannins are hydrolyzed to GA, the triphenol compounds, pyrogallic acid and GA have been reported to inhibit chemical mediator release from mast cells (Matsuo et al 1997;Kim et al 2006). It will be necessary to clarify the in vivo antiallergic activity of hydrolyzable tannins metabolites in the future.…”

“…The triphenol structure plays an important role in the inhibitory activity of tea polyphenols (Matsuo et al 1997). Furthermore, there was a significant correlation between inhibitions of chemical mediator release and DPPH or superoxide anion radical scavenging activities (Suzuki et al 2005).…”

Spectrofluorometric Determination of Catechins with 1,2-Diphenylethylenediamine

“…Various biological and pharmacological properties of tea catechins have been reported. In particular, EGCG is known to exhibit more potent beneficial effects than the other catechins, including antioxidative, 1,2 antimutagenic, 3 antimutagenic/anticarcinogenic, [4][5][6] antiallergenic, 7,8 and antiatherosclerotic properties. 9,10 Many published reports describe the determination and quantification of catechins.…”