Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production

Converti, Attílio; Casazza, Alessandro Alberto; Ortiz, Erika Y.; Perego, Patrizia; Borghi, Adriana Del

doi:10.1016/j.cep.2009.03.006

Cited by 1,175 publications

(625 citation statements)

References 32 publications

Supporting

Mentioning

504

Contrasting

Unclassified

Order By: Relevance

“…This shows that the behaviour of Chlorella when exposed to environmentally relevant concentrations of Cd may differ from that shown by Chlorella exposed to very high Cd concentrations. Converti et al (2009) demonstrated that a combination of temperature and nitrogen limitation caused the growth rate of C. vulgaris to decrease while total lipid content tripled, which agrees with our findings with the alga under combined Cd and N stress.…”

Section: Discussionsupporting

confidence: 82%

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Chia

Lombardi

Melão

et al. 2013

European Journal of Phycology

View full text Add to dashboard Cite

Investigations were carried out to evaluate lipid class and fatty acid composition of Chlorella vulgaris in relation to several combinations of cadmium (2 Â 10 -8 ; 10 À7 M Cd) and nitrogen (2.9 Â 10 À6 to 1.1 Â 10 À3 M N) concentrations. Triacylglycerols (TAG), acetone mobile polar lipids (AMPL) and phospholipids (PL) were the major lipid classes of C. vulgaris under all the tested conditions. TAG was the lipid class accumulated in the highest amount in N-limited C. vulgaris. The AMPL lipid class was lowest at the lowest N and highest Cd treatment. High proportions of saturated fatty acids (SAFA) and monounsaturated fatty acids (MUFA) in the total lipid extracts were recorded under N limitation in the presence of Cd. 16:1(n-11) was an exception among the MUFA as its levels decreased at low N. Total polyunsaturated FA (PUFA), !3 PUFA levels, and PUFA:SAFA ratios increased with increasing N concentrations, but when exposed to Cd, their levels were significantly reduced. Unlike the other PUFA, 18:2(n-6) increased with increasing N limitation. Significant negative relationships between TAG, MUFA and SAFA with N were recorded. Cd had a positive correlation with TAG, while N was with AMPL, PL and PUFA. In conclusion, a combination of N limitation and Cd stress significantly altered the lipid composition of C. vulgaris, and N limitation had the most significant overall effect on lipid class and fatty acid composition of the microalga.

show abstract

Section: Discussionsupporting

confidence: 82%

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Chia

Lombardi

Melão

et al. 2013

European Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…which is approximately twice the value of 5.9 g lipids per 100 g dry algae reported by Converti et al (2009) …”

Section: Resultssupporting

confidence: 61%

Growth and biochemical composition of Chlorella vulgaris in different growth media

Chia

Lombardi

Melão

2013

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

show abstract

“…At nitrogen-sufficient condition, i.e., N 1.5 , lipid peak is very small, may be due to high biomass production as compared to lipid synthesis. Studies by Converti et al (2009) on Chlorella vulgaris showed no significant difference in lipid production when cultivated in nitrogen- starved or nitrogen-limited conditions. Our experimental trail with different nitrogen levels showed less marked cell inhibition with high lipid production at N -0.5 .…”

Section: Ftir Studiesmentioning

confidence: 99%

Influence of varying nitrogen levels on lipid accumulation in Chlorella sp.

Kiran

Pathak

Kumar

et al. 2016

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Microalgal lipids can be enhanced through varying nitrogen (N) content, and limited supply of nitrogen source seems to be valuable approach for increased lipid accumulation in microalgae. In this study, Chlorella sp. IM-02 was observed under fluorescence microscope for increased number of lipid bodies under nitrogen scarcity. Fourier transform infrared spectroscopy was used to determine spectral changes due to varying lipid content under nitrogen-starved (N 0 , without sodium nitrate), nitrogen-limited (N 0.1 , N 0.25 , N 0.5 and N 1.0 representing 0.1, 0.25, 0.5 and 1.0 g/L of sodium nitrate, respectively) and nitrogen-sufficient (N 1.5 , i.e., 1.5 g/L sodium nitrate) setting. Chlorophyll content was also monitored under these conditions as growth indicator. Various biochemical components viz. total carbohydrates, total proteins and total lipids were also estimated under varying nitrogen levels spectrophotometrically. On fourth day itself, maximum lipid productivity was observed in case of N 0.5 , which is having one-third of nitrogen concentration present in original growth media, BG-11. This concludes N 0.5 as suitable nitrogen provision for better production of lipids in Chlorella sp. IM-02 without much compromising the biomass production as both growth and lipid quantity are key parameters affecting the lipid productivity of any microalgal strain.

show abstract

Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production

Cited by 1,175 publications

References 32 publications

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Growth and biochemical composition of Chlorella vulgaris in different growth media

Influence of varying nitrogen levels on lipid accumulation in Chlorella sp.

Contact Info

Product

Resources

About