Effect of Temperature Conditioning on Chilling Injury of Cucumber Cotyledons

Lafuente, Marı́a T.; Belver, Andrés; Guye, Michael G.; Saltveit, Mikal E.

doi:10.1104/pp.95.2.443

Cited by 129 publications

(80 citation statements)

References 25 publications

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“…Temperature also has an effect on endogenous ABA levels (8). However, the effect of a rapid increase in temperature or heat shock on leaf ABA concentration has not been widely studied (13).…”

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Wild-Type Levels of Abscisic Acid Are Not Required for Heat Shock Protein Accumulation in Tomato

Bray¹

1991

Plant Physiol.

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Levels of endogenous abscisic acid (ABA) in wild type were not required for the synthesis of heat shock proteins in detached leaves of tomato (Lycopersicon esculentum Mill., cv Ailsa Craig). Heat-induced alterations in gene expression were the same in the ABA-deficient mutant of tomato, flacca, and the wild type. Heat tolerance of the mutant was marginally less that the wild type, and in contrast, ABA applications significantly reduced the heat tolerance of wild-type leaves. It was concluded that elevated levels of endogenous ABA are not involved in the tomato heat shock response.ABA regulates many plant responses to the environment. Some of the functions of endogenous ABA have been demonstrated using mutants that are deficient in ABA. These mutants have impaired stomatal function (18), do not accumulate drought-induced gene products (3, 6), and do not develop cold hardiness (12) as a direct result of reduced ABA levels. Exogenous ABA treatments have been used to demonstrate the possible range of responses to the environment that ABA may control. ABA application enhances adaptation to various stresses such as recovery from heat shock, induction of freezing resistance in cell cultures, and acceleration of adaptation of cultured cells to salt stress (1,19). These results have led to the suggestion that ABA is a stress hormone and that it regulates stress responses common to many environmental stresses (2).The concentration of ABA increases in response to environmental stress. The greatest increase in ABA concentration has been observed in response to drought stress, yet other osmotic stresses such as salinity and PEG-mediated water deficit also result in elevated levels of ABA ( 19). Temperature also has an effect on endogenous ABA levels (8). However, the effect of a rapid increase in temperature or heat shock on leaf ABA concentration has not been widely studied (13).ABA applications mimic many changes in gene expression that occur during environmental stresses such as drought, salt, and cold stress. It has been demonstrated using an ABAdeficient mutant of tomato, flacca, during drought stress that 'This work was supported by

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Wild-Type Levels of Abscisic Acid Are Not Required for Heat Shock Protein Accumulation in Tomato

Bray¹

1991

Plant Physiol.

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“…11 O, 1996 ing temperature (Cabrera and Saltveit, 1990), or holding the tissue near the chilling temperature or above 35°C before chilling (Lafuente et al, 1991;Lurie and Klein, 1991;Saltveit, 1991). Temperature acclimation at low temperature has been found to correlate with the accumulation of a number of unique proteins (Guy et al, 1985;Mohapatra et al, 1989), and at elevated temperature HSPs accumulated (Lafuente et al, 1991;Lurie and Klein, 1991). The appearance of the HSPs coincided with increased chilling resistance.…”

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Reversible Inhibition of Tomato Fruit Gene Expression at High Temperature (Effects on Tomato Fruit Ripening)

et al. 1996

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caused by alterations in gene expression (Mansson et al., 1985). %ter et al. (1985) identified 19 different mRNA families that were present in ripe fruit but were undetectable or present at a much reduced level in unripe fruit. A number of cDNA clones prepared from these "As have been identified and thereby linked to ripening-related physiological changes. pTOM13 has been identified as a an enzyme that catalyzes the last step in the ethylene synthesis pathway. pTOM6 encodes for PG, an enzyme that depolymerizes pectins in the fruit cell wall (Grierson et 1986)' Phytoene 'ynthase, an enzyme in the pathway Of lycopene synthesist has been identified with pToM5 (Fray and Grierson, 1993). These clones have Provided a useful means to examine different factors that influence the ripening process.Short exposures to temperatures above 35°C have been found to inhibit fruit ripening reversibly (Biggs et al., 1988;Lurie and Klein, 1990), whereas fruit held for extended periods at high temperature ripen abnormally (ogura et al., 1975). rn tomatoes, elevated temperatures inhibit ethylene synthesis (Biggs et al,, 1988) and PG accumulation (Yoshida et al., 1984) and interfere with lycopene synthesis (Ogura et al., 1975). Some of these effects have been attrib-(Ogura et al.r 1975)r but and Grierson (1988) demonstrated that inhibition also occurred at the level of gene expression. If the inhibitory effect of high temperature is reversible, then fruit subjected to a heat stress might ripen normally but more slowly than unheated fruit. This was ened more slowly than unheated fruit (~~~i and Klein, were not investigated.Tomato fruit are chilling sensitive and have reduced storage life and decreased ability to ripen when stored at temperatures below 10°C; the degree of damage is related to the length of time at a particular temperature. Chilling injury could be reduced in temperature-sensitive tissues by modifying the pattern of temperature exposure, e.g. periodically warming the chilled tissue above the chillAbbreviations: HSP, heat-shock protein; PG, polygalacturonase.The reversible inhibition of three ripening-related processes by high-temperature treatment (38°C) was examined i n tomato (Lycopersicon esculentum 1. cv Daniella) fruit. Ethylene production, color development, and softening were inhibited during heating and recovered afterward, whether recovery took place at 20°C or fruit were first held at chilling temperature (2OC) after heating and then daced at 2ooc* Ethylene production and colar development proceeded normally in heated fruit after 14 d of chilling, whereas the unheated fruit had delayed ethylene production and uneven color development. Levels of mRNA for 1 -aminocyclopropane-1 -carboxylic acid oxidase, phytoene synthase, and polygalacturonase decreased dramatically during the heat treatment but recovered afterward, whereas the mRNA for HSPl7 increased during the high-temperature treatment and then decreased when fruit were removed from heat. As monitored by western blots, the HSp17 protein disappeared from frui...

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“…The concentrations for Chlorophyll a, Chlorophyll b, and the total leaf carotenoids (xanthophylls and carotenes) were calculated in mg/ml extract solution according to Lichtenthaler and Buschmann (2001). Electrolytes leakage was used as indicator for cell injury which determined by conductivity method according to Lafuente et al (1991) with some modification Camejo et al (2005) and Alsadon et al (2006) as follow: uniform leaf segments from the new leaves were taken from the marked plants at vegetative and fruiting stages, 30 and 75 days from transplanting, respectively. Four leaf disks of one cm in diameter from each plant were washed with distilled water three times and transferred in 20 ml tubes completed with distilled water and maintained for 20 hours at room temperature.…”

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confidence: 99%

Population Parameters and Path-Coefficient Analysis of Tomato Grown under Heat Stress

2017

Alexandria Science Exchange Journal: An International Quarterly

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Development of genetically improved heat-tolerant varieties is one of the most important approaches for minimizing the negative effects of heat stress. Although there are many factors causing similar negative effects of high temperature, screening of heat tolerance using fruit set percentage as a direct selection for promising genotypes is the common approach. This study aimed to enhancement of selection capacity for heat stress through dual trends; analysis the genetic and phenotypic information derived from generation analysis in tomato and study the causes and effects of indirect selection for heat stress by path coefficient analysis. Homogenous and heterogeneous generations (P 1 , P 2 , F 1 and F 2 ) were used for estimation the following traits; fruit weight (g), fruit set %, total yield per plant (kg), fruit firmness (inch/cm 2 ), chlorophyll a/b ratio, total chlorophyll/carotenoids ratio and electrolytes leakage at vegetative (ELV) and fruiting (ELF) stages. High genetic variability (GCV, 37.24) with moderate value of heritability (72.02%) was recorded by total chlorophyll/carotenoids followed by electrolytes leakage at vegetative stage (ELV) with genotypic coefficient of variance of 25.89% along with high heritability that estimated by 82.20%. Results of the path analysis along with heritability and selection gain showed that fruit set % and electrolytes leakage are the two most critical component traits for fruit yield under heat stress condition. On the other hand, the average fruit weight had low indirect effect but highly significant with fruit yield per plant. The electrolytes leakage showed high ability to distinguish the sensitive and tolerant tomato plants at early stage of plant development which can be relied upon in the selection of heat tolerant genotypes. Among the evaluated materials, ten genotypes; 130, 129, 127, 08, 11, 10, 131, 155, 161 and 124 that possessing better heat tolerance performance with high yield that could be incorporated in further tomato improvement programs.

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Effect of Temperature Conditioning on Chilling Injury of Cucumber Cotyledons

Cited by 129 publications

References 25 publications

Wild-Type Levels of Abscisic Acid Are Not Required for Heat Shock Protein Accumulation in Tomato

Wild-Type Levels of Abscisic Acid Are Not Required for Heat Shock Protein Accumulation in Tomato

Reversible Inhibition of Tomato Fruit Gene Expression at High Temperature (Effects on Tomato Fruit Ripening)

Population Parameters and Path-Coefficient Analysis of Tomato Grown under Heat Stress

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