Effect of temperature on the hydrolysis of levan treated with compressed hot water fluids

Shimizu, Naoto; Abea, Andrés; Ushiyama, Tetsuya; Öner, Ebru Toksoy

doi:10.1002/fsn3.1488

Cited by 6 publications

(5 citation statements)

References 33 publications

(42 reference statements)

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“…Both BLs show a crystalline morphology with a smooth and glossy surface at the splitting site, forming sharp edges. Similar surface features were reported by Shimizu et al [64] and Pei et al [65] for BLs isolated from Erwinia herbicola and Bacillus megaterium PFY-147, respectively. A cross-section of specimens revealed a poroid, concrete-like network structure with less than 3 µm diameter of pores within matrices of BL isolated from S. salivarius K12 (B) and L. mesenteroides DSM 20343.…”

Section: Surface Morphology and Microstructural Feature Analysis Of B...supporting

confidence: 86%

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

Radenkovs,

Valdovska,

Galina

et al. 2023

Nanomaterials

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Considering the documented health benefits of bacterial exopolysaccharides (EPSs), specifically of bacterial levan (BL), including its intrinsic antimicrobial activity against certain pathogenic species, the current study concentrated on the development of active pharmaceutical ingredients (APIs) in the form of colloid systems (CoSs) containing silver nanoparticles (AgNPs) employing in-house biosynthesized BL as a reducing and capping agent. The established protocol of fermentation conditions implicating two species of lactic acid bacteria (LAB), i.e., Streptococcus salivarius K12 and Leuconostoc mesenteroides DSM 20343, ensured a yield of up to 25.7 and 13.7 g L−1 of BL within 72 h, respectively. An analytical approach accomplished by Fourier-transform infrared (FT-IR) spectroscopy allowed for the verification of structural features attributed to biosynthesized BL. Furthermore, scanning electron microscopy (SEM) revealed the crystalline morphology of biosynthesized BL with a smooth and glossy surface and highly porous structure. Molecular weight (Mw) estimated by multi-detector size-exclusion chromatography (SEC) indicated that BL biosynthesized using S. salivarius K12 has an impressively high Mw, corresponding to 15.435 × 104 kilodaltons (kDa). In turn, BL isolated from L. mesenteroides DSM 20343 was found to have an Mw of only 26.6 kDa. Polydispersity index estimation (PD = Mw/Mn) of produced BL displayed a monodispersed molecule isolated from S. salivarius K12, corresponding to 1.08, while this was 2.17 for L. mesenteroides DSM 20343 isolate. The presence of fructose as the main backbone and, to a lesser extent, glucose and galactose as side chain molecules in EPS hydrolysates was supported by HPLC-RID detection. In producing CoS-BL@AgNPs within green biosynthesis, the presence of nanostructured objects with a size distribution from 12.67 ± 5.56 nm to 46.97 ± 20.23 was confirmed by SEM and energy-dispersive X-ray spectroscopy (EDX). The prominent inhibitory potency of elaborated CoS-BL@AgNPs against both reference test cultures, i.e., Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, and Staphylococcus aureus and those of clinical origin with multi-drug resistance (MDR), was confirmed by disc and well diffusion tests and supported by the values of the minimum inhibitory and bactericidal concentrations. CoS-BL@AgNPs can be treated as APIs suitable for designing new antimicrobial agents and modifying therapies in controlling MDR pathogens.

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Section: Surface Morphology and Microstructural Feature Analysis Of B...supporting

confidence: 86%

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

Radenkovs,

Valdovska,

Galina

et al. 2023

Nanomaterials

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“…Samples were place on aluminum stub coated with gold palladium alloys and equal sided carbon tape employing an ion sputtering device (e101 Ion Sputter, Hitachi, Ltd, Tokyo, Japan) before SEM investigation. Investigation was done under high vacuum conditions, <1.0 × 10 −3 Pa, at a voltage of 10 kV [31]. The liquid fractions were analyzed using the high-performance liquid chromatography-refraction index (HPLC-RI) 1200 Infinity series with Shodex KS-802 column (Showa Denko, K.K, Tokyo, Japan).…”

Section: Characterization Of Raw Corn Stover and Products Of Htcmentioning

confidence: 99%

“…A solution of 0.3% pullulan standard was used to perform for an alignment peak. The injected volume was 100 µL [31]. Gas chromatography (GC-4000, GL Science, Tokyo, Japan) was used to analyze the gas fraction.…”

Section: Characterization Of Raw Corn Stover and Products Of Htcmentioning

confidence: 99%

Investigating the Effect of Processing Parameters on the Products of Hydrothermal Carbonization of Corn Stover

Mohammed

Kushima

et al. 2020

Sustainability

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Corn stover is an abundant and underused source of lignocellulose waste biomass that can be transformed into a high-quality energy resource using hydrothermal carbonization (HTC). This investigation has focused on the effect of processing parameters on the products of HTC—namely solid fuel or hydrochar and liquid and gas fractions. HTC was conducted in a temperature-controlled small batch reactor with corn stover and deionized water under oxygen-free conditions obtained by pressurizing the reactor headspace with nitrogen gas. The properties of the hydrochar and liquid and gas fractions were evaluated as a function of the process temperature (250–350 °C), residence time (30–60 min) and biomass/water ratio (0.09–0.14). Central composite design modules in a response surface methodology were used to optimize processing parameters. The maximum mass yield, energy yield and high heating value (HHV) of the hydrochar produced were 29.91% dry weight (dw), 42.38% dw and 26.03 MJ/kg, respectively. Concentrations of acetic acid and hydrogen gas were 6.93 g/L and 0.25 v/v%, respectively. Experimental results after process optimization were in satisfactory agreement with the predicted HHV. The optimal HTC process parameters were determined to be 305 °C with a 60 min residence time and a biomass/water ratio of 0.114, yielding hydrochar with a HHV of 25.42 MJ/kg. The results confirm the feasibility of an alternative corn stover management system.

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“…Levan has prebiotic properties [ 88 ], promoting the growth of beneficial intestinal bacteria ( Figure 5 ) [ 89 ]. It exhibits high thermal resistance, which makes it possible to process at high temperatures [ 90 ]. Recent research indicates the prebiotic properties of levan obtained via enzymatic synthesis.…”

Section: Properties and Application Of Levanmentioning

confidence: 99%

Recent Developments and Applications of Microbial Levan, A Versatile Polysaccharide-Based Biopolymer

Domżał-Kędzia

Ostrowska²,

Lewińska

et al. 2023

Molecules

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Polysaccharides are essential components with diverse functions in living organisms and find widespread applications in various industries. They serve as food additives, stabilizers, thickeners, and fat substitutes in the food industry, while also contributing to dietary fiber for improved digestion and gut health. Plant-based polysaccharides are utilized in paper, textiles, wound dressings, biodegradable packaging, and tissue regeneration. Polysaccharides play a crucial role in medicine, pharmacy, and cosmetology, as well as in the production of biofuels and biomaterials. Among microbial biopolymers, microbial levan, a fructose polysaccharide, holds significant promise due to its high productivity and chemical diversity. Levan exhibits a wide range of properties, including film-forming ability, biodegradability, non-toxicity, self-aggregation, encapsulation, controlled release capacity, water retention, immunomodulatory and prebiotic activity, antimicrobial and anticancer activity, as well as high biocompatibility. These exceptional properties position levan as an attractive candidate for nature-based materials in food production, modern cosmetology, medicine, and pharmacy. Advancing the understanding of microbial polymers and reducing production costs is crucial to the future development of these fields. By further exploring the potential of microbial biopolymers, particularly levan, we can unlock new opportunities for sustainable materials and innovative applications that benefit various industries and contribute to advancements in healthcare, environmental conservation, and biotechnology.

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Effect of temperature on the hydrolysis of levan treated with compressed hot water fluids

Cited by 6 publications

References 33 publications

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

Investigating the Effect of Processing Parameters on the Products of Hydrothermal Carbonization of Corn Stover

Recent Developments and Applications of Microbial Levan, A Versatile Polysaccharide-Based Biopolymer

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