Effect of Th1/Th2 cytokine administration on proinflammatory SKOV-3 cell activation

Mielczarek‐Palacz, Aleksandra; Sikora, Justyna; Kondera-Anasz, Zdzisława; Mickiewicz, Patrycja; Mickiewicz, Adam

doi:10.5114/aoms.2015.53143

Cited by 8 publications

(12 citation statements)

References 39 publications

(45 reference statements)

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“…In addition, several studies have demonstrated that the tumor cell line SKOV3 in the presence of PBMC causes the production of high concentration of IL-6 released by both SKOV3 cell line and PBMC. They also indicate that the interaction of these two types of cells provokes greater IL-6 production [48,58].…”

Section: Discussionmentioning

confidence: 93%

Increased Tumor Necrosis Factor-alpha Expression as an Inflammatory Response Indicator and a Cancer Risk in Polycystic Ovarian Syndrome

et al. 2018

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Background: Polycystic Ovarian Syndrome (PCOS) is a pro-inflammatory condition that supports the development of metabolic aberration and ovarian dysfunction. Chronic inflammation and the increased levels of androgens in this group of patients and their impact on the immune system may increase the risk of developing malignancies, including ovarian cancer. Thus, we interacted ovarian tumor cells with Peripheral Blood Mononuclear Cells (PBMC) to evaluate some of their responses to the tumor microenvironment. Materials and Methods: PBMC were collected from 25 patients with PCOS and 25 healthy women and isolated by Ficoll density gradient centrifugation. Then, we measured cell proliferation and Tumor Necrosis Factor-alpha (TNF-α) concentration at different time intervals (48 and 72 hours) after cocultivation of ovarian tumor cell lines (SKOV3, A2780) with PBMC in an indirect contact transwell system. Results: The proliferative response of executive cells during stimulation with tumor cell lines demonstrated no statistically significant difference between the patients and healthy groups, despite lower mean score in the control group. The proliferation rate after 72 h was significantly higher than that of the 48-h interval (P<0.01). The production of TNF-α in the co-culture of the A2780 cell line significantly increased in the patients at different time intervals compared to the controls (P<0.05). Conclusion: We observed an increased proliferative response of effector cells and TNF-α production in PCOS patients compared to healthy individuals. This suggests a low grade of chronic inflammation that is the immunological feature of the ovary in PCOS patients. However, an increased risk of cancer in patients with PCOS requires further in vitro investigation of other aspects of anti-tumor responses using diverse sample size. Additional, exploring other immune cytokine profiles could be beneficial.

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Section: Discussionmentioning

confidence: 93%

Increased Tumor Necrosis Factor-alpha Expression as an Inflammatory Response Indicator and a Cancer Risk in Polycystic Ovarian Syndrome

et al. 2018

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“…preterm labor, preeclampsia, fetal Down could be the aggressive biological nature of ovarian cancer whereby high levels of S1P produced by the tumor promote cell proliferation and cell transformation as well as survival of abnormal cells, neoangiogenesis and inhibition of pro-apoptotic processes. Currently, researchers are searching for useful markers in the advanced diagnosis of ovarian cancer [33,34]. Angiogenesis plays a crucial role in the tumor transformation, invasion and spread of ovarian cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Blood bioactive sphingolipids in patients with advanced serous epithelial ovarian cancer – mass spectrometry analysis

et al. 2021

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Introduction: Due to the lack of highly specific and sensitive methods for diagnosing ovarian cancer at advanced stages (according to the International Federation of Gynecology and Obstetrics [FIGO] classification stage III-IV), new noninvasive biomarkers are urgently needed. This study aims to investigate how the levels of plasma bioactive sphingolipids (ceramides, sphingosine-1-phosphate, sphingosine and sphinganine) are altered in serum, erythrocytes and platelets of patients with advanced serous ovarian cancer. Material and methods: A total of 135 patients with advanced serous ovarian cancer and 159 women with normal ovarian morphology were enrolled.

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“…The involvement of T‐helper (Th) cells represents S. schenckii adaptive immunity which involves Th1‐type cytokine response in the early stage of the inflammatory reaction, while an elevated Th2‐type cytokine response promotes fungal infections in the late stage. Pro‐inflammatory cytokines can mediate the Th1‐type response with IL‐1 and TNF‐a 11 . The Th2‐type response is mainly characterized by the subsequent anti‐inflammatory cytokines IL‐4 and IL‐10.…”

Section: Discussionmentioning

confidence: 99%

“…Proinflammatory cytokines can mediate the Th1-type response with IL-1 and TNF-a. 11 The Th2-type response is mainly characterized by the…”

mentioning

confidence: 99%

CD4 ⁺ T cells apoptosis and conversion of Th1/Th2‐type cytokines promote the progress of sporotrichosis

Mei

Jia

et al. 2021

Dermatologic Therapy

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Sporotrichosis is a fungal disease of the human and other mammals, caused by a complex of Sporothrix schenckii. The disease follows the traumatic inoculation to lead to fixed lesions, regional lymphangitic lesions, or even disseminated lesions including internal involvement, which depends on host immunological status and strain virulence. In this work, we observed the role of CD4 + T cells apoptosis and conversion of Th1/Th2-type cytokines in the cellular immunity regulation on mice model sporotrichosis. The experiments showed that there was more CD4 + T cells apoptosis, by endogenous apoptosis signaling pathway (P < .05), and more conversions of Th1/Th2-type cytokines in more severe and longer duration groups (P < .05). Meanwhile, the trends of the conversions of Th1/Th2-type cytokines were almost consistent with the CD4 + T cell's apoptosis in the corresponding groups. These findings suggest that CD4+ T cells apoptosis and conversion of Th1/Th2-type cytokines are contributing to promoting the progress of sporotrichosis.

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Effect of Th1/Th2 cytokine administration on proinflammatory SKOV-3 cell activation

Cited by 8 publications

References 39 publications

Increased Tumor Necrosis Factor-alpha Expression as an Inflammatory Response Indicator and a Cancer Risk in Polycystic Ovarian Syndrome

Increased Tumor Necrosis Factor-alpha Expression as an Inflammatory Response Indicator and a Cancer Risk in Polycystic Ovarian Syndrome

Blood bioactive sphingolipids in patients with advanced serous epithelial ovarian cancer – mass spectrometry analysis

CD4 ⁺ T cells apoptosis and conversion of Th1/Th2‐type cytokines promote the progress of sporotrichosis

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Effect of Th1/Th2 cytokine administration on proinflammatory SKOV-3 cell activation

Cited by 8 publications

References 39 publications

Increased Tumor Necrosis Factor-alpha Expression as an Inflammatory Response Indicator and a Cancer Risk in Polycystic Ovarian Syndrome

Increased Tumor Necrosis Factor-alpha Expression as an Inflammatory Response Indicator and a Cancer Risk in Polycystic Ovarian Syndrome

Blood bioactive sphingolipids in patients with advanced serous epithelial ovarian cancer – mass spectrometry analysis

CD4 + T cells apoptosis and conversion of Th1/Th2‐type cytokines promote the progress of sporotrichosis

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CD4 ⁺ T cells apoptosis and conversion of Th1/Th2‐type cytokines promote the progress of sporotrichosis