Effect of the Extract of Endophytic fungus, Nigrospora sphaerica CL-OP 30, Against the Growth of Methicillin- Resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; (MRSA) and &lt;i&gt;Klebsiella pneumonia&lt;/i&gt; cells

In the present study, an endophytic fungus with a considerable antimicrobial activity was isolated from the medicinal plant Mentha Pulegium L. The morphological and molecular identification revealed that this fungus is a Penicillium griseofulvum. A preliminary screening was done to choose the suitable culture medium for a maximum production of the bioactive compounds using the dual-culture agar diffusion assay. The malt extract agar (MEA) and potato dextrose agar (PDA) media were the suitable, and the MEA was selected for the further study. Extraction was done with three solvents, n-Hexane, dichloromethane and ethyl acetate, and the crude extracts were tested against Gram-positive and negative bacteria. A high activity was found with ethyl acetate and dichloromethane crude extracts against all bacteria with a maximal inhibition zones of 45.5 and 41 mm respectively obtained against Escherichia coli. The minimum inhibitory and bactericidal concentrations (MIC, MBC) of ethyl acetate crude extract were evaluated using the broth micro-dilution method. A MIC of 50 µg / ml on Gramnegative bacteria and of 100 µg / ml on Gram-positive bacteria was found. The MBC S ranged from 50 and 200 µg/ml. The time kill study has revealed a bactericidal activity of the Penicillium griseofulvum crude extract. At 24 h and for all concentrations (MIC× 2 and MIC× 4), 100% killing of the bacterial cells was achieved. These results prove that the extract of Penicillium griseofulvum can be a promising source of important bioactive molecules.

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“…Where, V0 is the initial viable cell count and Vz is the viable cell count at time z (Ibrahim et al, 2015).…”

Section: Time Kill Studymentioning

confidence: 99%

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Zerroug¹,

Sadrati²,

Demirel³

et al. 2018

Afr. J. Microbiol. Res.

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“…This finding correlates with that of Okemo et al [21], who found that Ximenia caffra extracts kill S. aureus in both a time-and concentration-dependent manner. Also, extracts of the endophytic fungus Nigrospora sphaerica CL-OP30 are active against S. aureus in a time-and concentrationdependent manner during the first 12 h of treatment [22].…”

Section: Discussionmentioning

confidence: 99%

“…Similar results have been described in a study where S. aureus cells were treated with Nigrospora sphaerica CL-OP30 extract. The authors attributed the formation of cavities, surface alterations, and appearance of collapsed and shrunken cells to leakage of cytoplasmic contents [22].…”

Section: Discussionmentioning

confidence: 99%

Time-kill profiles and cell-surface morphological effects of crude Polycephalomyces nipponicus Cod-MK1201 mycelial extract against antibiotic-sensitive and -resistant <i>Staphylococcus aureus</i>

Thammawat¹,

Sangdee²,

Sangdee³

2017

Trop. J. Pharm Res

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“…Besides that, the MRSA cells were observed to be in crumpled and shrunken stages. Ibrahim [41], stated that the reason of the irregular shape of bacterial cell treated with fungal extract (such as cell surface crumpled and shrinkage of the cells) could be due to extensive loss of cell organelles and also the leakage of cytoplasm, supported by the damage of cell envelope.…”

Section: Discussionmentioning

confidence: 99%

Anti-Mrsa of the Ethyl Acetate Crude Extract From Lasiodiplodia Pseudotheobromae Ibrl Os-64, an Endophytic Fungus Isolated From Leaf of Ocimum Sanctum Linn

Taufiq

Demirtaş

2018

Int J Pharm Pharm Sci

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Objective: To investigate the effects of ethyl acetate crude extract of an endophytic fungus, L. pseudotheobromae IBRL OS-64 which was isolated from leaf of Ocimum sanctum against the growth of methicillin-resistant Staphylococcus aureus (MRSA)., a common pathogenic bacteria to human. Methods:Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. Time-kill assay was used to examine the effect of the extract on the MRSA growth profile. The effects of the extract on the ultrastructure of MRSA cells were analyzed by scanning electron microscopic (SEM) study. Results:The results obtained from this study showed the fungal ethyl acetate crude extract exhibited a strong bactericidal effect on MRSA where the ratio of MBC/MIC was 2 and less than 4. The MIC and MBC values were 125.0 and 250.0 µg/ml, respectively. The time-kill study revealed that the bacteriocidal activity of the extract was both concentration and time-dependent. After 12 h treatment, the interaction of extract with MRSA cells resulted in the formation of dents, cavity or small dimples on the cell surface, indicating disintegration of the cell wall and cell membrane that resulting in leakage of their cytoplasmic contents, and ultimately cell death. Conclusion:The ethyl acetate crude extract of L. pseudotheobromae IBRL OS-64 showed a significant anti-MRSA activity and principally affected the cell wall and the cell membrane of the growing MRSA cells. This is the first report on L. pseudotheobromae, an endophytic fungus isolated from medicinal herb, Ocimum sanctum Linn.

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Effect of the Extract of Endophytic fungus, Nigrospora sphaerica CL-OP 30, Against the Growth of Methicillin- Resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Klebsiella pneumonia</i> cells

Cited by 29 publications

References 16 publications

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Time-kill profiles and cell-surface morphological effects of crude Polycephalomyces nipponicus Cod-MK1201 mycelial extract against antibiotic-sensitive and -resistant <i>Staphylococcus aureus</i>

Anti-Mrsa of the Ethyl Acetate Crude Extract From Lasiodiplodia Pseudotheobromae Ibrl Os-64, an Endophytic Fungus Isolated From Leaf of Ocimum Sanctum Linn

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