Effect of the Hsp90 modulators on the heat-shock response in eukaryotic cells

Papamichael, Konstantinos; Vovou, I.; Miligkos, V.; Stavrinidis, E.; Delitheos, A.; Tiligada, Ekaterini

doi:10.1007/bf02931447

Cited by 5 publications

(14 citation statements)

References 17 publications

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“…Other compounds, such as 17‐ β ‐estradiol (Papamichael et al . ), failed to confer resistance to a subsequent HS, similar to dimethindene and ranitidine that were tested in this study. Taken together, these results provide supportive evidence for stimulus‐related induction and differential modulation of the adaptive response by small molecules such as drugs and are consistent with the model that cross‐stress protection occurs via diverse responses to the original stressor (Berry and Gasch ).…”

Section: Discussionmentioning

confidence: 78%

“…), prednisolone and the Hsp90 inhibitor geldanamycin (Papamichael et al . ). On the contrary, acquisition of thermotolerance by the phosphatase inhibitor sodium molybdate did not rely on nascent protein synthesis (Tiligada et al .…”

Section: Discussionmentioning

confidence: 97%

“…Thus, in addition to histamine (Delitheos et al 2010), de novo protein synthesis-dependent induction of thermotolerance has been observed following exposure to various agents such as anticancer drugs (Miligkos et al 2000;Tiligada et al 2006b), prednisolone and the Hsp90 inhibitor geldanamycin (Papamichael et al 2006). On the contrary, acquisition of thermotolerance by the phosphatase inhibitor sodium molybdate did not rely on nascent protein synthesis (Tiligada et al 1999).…”

Section: H X R Ligandsmentioning

confidence: 99%

“…Further to the challenge faced by molecular biology and genetics to determine the players of the stress response, the identification of modulating agents is of considerable importance to human health (Papamichael et al . ; Neef et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…The budding yeast Saccharomyces cerevisiae is a versatile and powerful model system for the investigation of the cellular stress response (Mager and Ferreira 1993;Tiligada et al 2002;Berry and Gasch 2008;Morano et al 2012), and it has been used as a drug discovery platform to identify compounds capable of modifying the response (Neef et al 2012). Further to the challenge faced by molecular biology and genetics to determine the players of the stress response, the identification of modulating agents is of considerable importance to human health (Papamichael et al 2006;Neef et al 2012). Thus, the manipulation of the response emerges as a promising target for the management of various diseases, including inflammatory disorders (Morimoto 2008;Westerheide et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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A subset of histamine receptor ligands improve thermotolerance of the yeast Saccharomyces cerevisiae

2012

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Aims: Histamine interacts with the stress response in eukaryotes. This study investigated the effects of antihistamines on the heat shock (HS) response in yeast, thereby exploring their functions in a well-established histamine receptor (H x R)-free model. Methods and Results: Stress response was evaluated by determining growth and viability of postlogarithmic phase grown yeast cultures after HS at 53°C for 30 min. The effects of H x R ligands were investigated following short-and long-term administration. The H 1 R antagonist dimethindene exerted doserelated antifungal actions, whereas the H 2 R antagonist ranitidine failed to elicit any effect. In contrast, the H 3/4 R and H 4 R ligands, thioperamide and JNJ7777120, respectively, induced the thermotolerant phenotype. The circumvention of thermotolerance by cycloheximide and the induction of Hsp70 and Hsp104 expression indicated the contribution of de novo protein synthesis in the adaptive process, likely directed towards alterations in Hsp expression. Conclusions: The data provide evidence for the differential function of H x R ligands in thermotolerance induction in yeast. Significance and Impact of the Study: First demonstration of the action of antihistamines in the HS response in yeast. The work supports the potential H x R-independent functions of histaminergic compounds in fungal adaptation and stimulates research on the prospect of their exploitation in eukaryotic (patho)physiology.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 97%

Section: H X R Ligandsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A subset of histamine receptor ligands improve thermotolerance of the yeast Saccharomyces cerevisiae

2012

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Histamine modulates the cellular stress response in yeast

2009

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The cellular stress response is a universal protective reaction to adverse environmental or microenvironmental conditions, such as heat and drugs, associated in part with the highly conserved heat shock proteins (HSPs). Histamine is a key inflammatory mediator derived from L: -histidine that governs vital cellular processes beyond inflammation, while recent evidence implies additional actions in both prokaryotes and eukaryotes. This study explored the possible role of histamine in the heat shock response in yeast, an established experimental model for the pharmacological investigation of the cellular stress response. The response was evaluated by determining growth and viability of post-logarithmic phase grown yeast cultures after heat shock at 53 degrees C for 30 min. Thermal preconditioning at 37 degrees C for 2 h served as a positive control. The effect of histamine was investigated following long-term administration through the post-logarithmic phase of growth or short-term administration for 2 h prior to heat shock. Short-term treatment with 1 mM histamine resulted in de novo protein synthesis-dependent acquisition of thermotolerance, while lower doses or long-term administration of histamine failed to induce the heat-resistant phenotype. Preliminary investigation of HSP104, HSP70 and HSP60 expression by western blotting showed an increase of these proteins after thermal preconditioning. However, a differential HSP and tubulin expression appeared to underlie the response of yeast cells to histamine. In conclusion, histamine was capable of inducing the adaptive phenotype, while the contribution of HSPs and tubulin and the potential implications remain largely elusive.

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l-Thyroxine induces thermotolerance in yeast

Papamichael

Delitheos

Mourouzis

et al. 2019

Cell Stress and Chaperones

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The cellular stress response (CSR) is a universal inducible reaction modulated, among others, by heat, drugs, and hormones. We aimed to investigate the role of L-thyroxine (T4) on the heat shock (HS) response in Saccharomyces cerevisiae. The CSR was evaluated by determining growth and viability of post-logarithmic phase grown yeast cultures after HS at 53°C for 30 min. We found that long-term T4 exposure can induce a dose-dependent and Hsp90 and H + trafficking-related thermotolerance in yeast.

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Effect of the Hsp90 modulators on the heat-shock response in eukaryotic cells

Cited by 5 publications

References 17 publications

A subset of histamine receptor ligands improve thermotolerance of the yeast Saccharomyces cerevisiae

A subset of histamine receptor ligands improve thermotolerance of the yeast Saccharomyces cerevisiae

Histamine modulates the cellular stress response in yeast

l-Thyroxine induces thermotolerance in yeast

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