Effect of time after hormonal stimulation on semen quality indicators of common carp, &lt;I&gt;Cyprinus carpio&lt;/I&gt; (Actinopterygii: Cypriniformes: Cyprinidae)

Cejko, Beata Irena; Kowalski, Radosław Kajetan; Kucharczyk, Dariusz; Żarski, Daniel; Targońska, Katarzyna

doi:10.3750/aip2011.41.2.01

Cited by 15 publications

(20 citation statements)

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“…Hormonal stimulation leads to increased reproductive efficiency of male broodstock and guarantees the synchronization of ovulation and spermiation and is a good starting point to solving problem with gametes maturation (Warnecke and Pluta 2003;Kucharczyk et al 2008). In common carp males, the application of Ovopel (1 pellet kg -1 ) enables semen to be obtained even after 72 h, but the semen volume and sperm number at this time are significantly lower than values noted after 24 h (Cejko et al 2011). This indicates that in common carp males, latency time is rather short and semen should be taken no longer than 24 h after hormonal treatment (Toth et al 1995;Christ et al 1996;Cejko et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In common carp males, the application of Ovopel (1 pellet kg -1 ) enables semen to be obtained even after 72 h, but the semen volume and sperm number at this time are significantly lower than values noted after 24 h (Cejko et al 2011). This indicates that in common carp males, latency time is rather short and semen should be taken no longer than 24 h after hormonal treatment (Toth et al 1995;Christ et al 1996;Cejko et al 2011). The presented study indicated that in comparison to the first Ovopel treatment, the repeated stimulation of males with this preparation resulted in decreased semen volume and total number of sperm.…”

Section: Discussionmentioning

confidence: 99%

“…metoclopramide] Ovopel (1 pellet kg -1 ). After 24-h post-hormonal application on 30 May, the fish were taken from the tanks (Cejko et al 2011) and floy-tagged, and then semen was collected (first semen sampling) with sterile syringes by abdominal massage. The fish were anesthetized with 2-phenoxyethanol (Merc, Darmstadt, Germany) at a dose of 0.5 ml l -1 of water.…”

Section: Semen Collection and Broodstock Manipulationmentioning

confidence: 99%

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Sperm quality and selected biochemical markers of seminal plasma at the beginning of the reproductive period of common carp, Cyprinus carpio L.

et al. 2013

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Changes in semen quality and selected biochemical markers were analyzed during a week of spawning season of common carp Cyprinus carpio L. Semen was obtained twice, on May 30 and on June 7, and each time it was collected 24 h after hormonal stimulation using Ovopel [(D-Ala 6 , Pro 9 NEt)-mGnRH ? metoclopramide] in 1 pellet kg -1 . The total volume of semen (ml), volume of semen per kg of body weight (ml kg -1 b.w.), sperm concentration (910 9 ml -1 ), total number of sperm per kg of body weight (910 9 kg -1 b.w.), pH of semen, pH of seminal plasma, seminal plasma osmotic pressure (mOsm kg -1 ) and the total protein content in seminal plasma (mg ml -1 ) were determined. A 10 mM Tris buffer containing 100 mM NaCl with 0.5 % BSA (pH 9.0, osmolality 200 mOsm kg ), movement linearity (LIN, %), wobbling index (WOB, %), amplitude of lateral head displacement (ALH, lm) and beat cross-frequency (BCF, Hz). The volume of semen per kg of BW, total number of sperm per kg of BW and semen pH were significantly lower at the second semen sampling compared to the first semen sampling. Volume of semen at the second sampling correlated positively with CASA parameters. A lack of differences among CASA parameters between both collection periods indicates good quality of carp sperm hormonally stimulated with Ovopel twice at a 1-week interval.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Semen Collection and Broodstock Manipulationmentioning

confidence: 99%

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Sperm quality and selected biochemical markers of seminal plasma at the beginning of the reproductive period of common carp, Cyprinus carpio L.

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“…Sperm concentration (910 9 ml À1 ) was determined in accordance with the procedure described by Cejko et al (2011). The amount of milt was recorded.…”

Section: Determination Of the Volume Of Milt And Sperm Countmentioning

confidence: 99%

“…The strong mechanism blocking the release of gonadotropins in cyprinids was confirmed and, therefore, complex preparations enriched with substances which have an antagonistic effect on dopamine are in use. A commonly used synthetic preparation to aid-common carp reproduction in hatcheries is [(D-Ala 6 , Pro 9 NEt)-metoclopramide], commercially named Ovopel (Horv ath et al, 1997;Brzuska and Białowa z s, 2002;Kucharczyk et al, 2008;Cejko et al, 2011). This preparation is available as pellets containing 18-20 lg mammalian mGnRHa and 8-10 mg metoclopramide (Horv ath et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Biochemical factors of common carpCyprinus carpioL. 1758, seminal plasma and its relationship with sperm motility parameters

et al. 2015

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Summary Compared were the volume and quality of carp Cyprinus carpio L. obtained from semen of males not subjected to hormonal stimulation (N = 10) but injected with 0.9% NaCl in the amount of 1 ml kg−1 b.w., as well as those stimulated using Ovopel (N = 10) in the amount of 1 granule kg−1 b.w. Semen was collected from the males 24 h after injection of hormonal preparation, to determine the pH, total volume (TVS) and volume recalculated per kg of body mass, concentration and total sperm production (TSP). Differences in sperm motility parameters between fish groups were determined by a computer‐assisted sperm analysis (CASA) system. Sperm motility was activated using 10 mm Tris buffer, containing 100 mm NaCl (pH 9.0, osmolality 200 mOsm kg−1). The pH, osmolality, total protein concentration and activity of acidic phosphatase (AcP), lactate dehydrogenase (LDH), ß‐N‐acetylglucosaminidase and arylsulfatase (AS) were determined in the seminal plasma of both test groups. The stimulation of male carp using Ovopel did not significantly affect CASA parameters, but did lead to an over ten‐fold increase in the values of TVS and VOS and nearly six times higher values of TSP as compared to the Control group. A significant increase in pH following stimulation with Ovopel indicates that cyclic AMP increased, thanks to which sperm acquire a capacity to move during their passage through the sperm ducts. An increase in the values of CASA parameters is a likely reflection of these changes.

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Effect of albumin and casein supplementation on the common carp Cyprinus carpio L. sperm motility parameters measured by CASA

et al. 2013

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Effect of time after hormonal stimulation on semen quality indicators of common carp, <I>Cyprinus carpio</I> (Actinopterygii: Cypriniformes: Cyprinidae)

Cited by 15 publications

References 29 publications

Sperm quality and selected biochemical markers of seminal plasma at the beginning of the reproductive period of common carp, Cyprinus carpio L.

Sperm quality and selected biochemical markers of seminal plasma at the beginning of the reproductive period of common carp, Cyprinus carpio L.

Biochemical factors of common carpCyprinus carpioL. 1758, seminal plasma and its relationship with sperm motility parameters

Effect of albumin and casein supplementation on the common carp Cyprinus carpio L. sperm motility parameters measured by CASA

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