Effect of traditional Chinese medicinal enemas on ulcerative colitis of rats

Abstract

AIM:To investigate the effects of traditional Chinese medicinal enema (TCME) on inflammatory and immune response of colonic mucosa of rats with ulcerative colitis (UC), and to observe the pathogenic mechanism. METHODS:Thirty UC rats, induced by intestinal enema together with 2.4-dinitrochlorobenzene (DNCB) and acetic acid, were randomly divided into 3 groups, i.e., GI, GII and GIII. Groups GI and GII were administered with TCME and salazosulfapyridine enema (SASPE), respectively. Group GIII was clystered with only normal saline (NSE), served as control. Group GIV was taken from normal rats as reference, once daily, from the 7th day after the establishment of UC for total 28 d. Interleukin-6 (IL-6) in the colonic mucosa was assayed by (16.28±0.19%) were raised. There was no significant difference between groups GI and GIV, but the difference between groups GI and GII was quite obvious (P<0.05). The expressions of TNF-α mRNA and IFN-γ mRNA in group GIII were much higher than those of group GIV, but those in group GI were significantly suppressed by TCME therapy. CONCLUSION:Ulcerative colitis is related to colonic regional mucosal inflammatory factors and immune imbalance. TCME can effectively inhibit regional mucosal inflammatory factors and improve their disorder of immunity.Guo SM, Tong HB, Bai LS, Yang W. Effect of traditional Chinese medicinal enemas on ulcerative colitis of rats.

Inhibition of MAP kinases by crude extract and pure compound isolated from Commiphora mukul leads to down regulation of TNF-α, IL-1β and IL-2

“…It has been reported that plant extracts or compounds that show inhibitory effects on these inflammatory cytokines are known to reduce the severity in diseases such as Crohn's disease, multiple sclerosis, RA, etc. [24]. When the profile of cytokine secretion by activated T cells and macrophages were analysed it was revealed that both crude ethyl acetate extract and pure compound of C. mukul significantly suppressed inflammatory cytokines such as IFN, IL-12, TNF-a, IL-1h cytokines (Fig.…”

Effect ofScutellariae Radixextract on experimental dextran-sulfate sodium-induced colitis in rats

“…Interestingly, a recent survey showed that one-third of both Chinese and Caucasian IBD patients had used complementary and alternative medicines and therapies [4] . Several TCM formulae have been shown to possess an anti-colitic effect in rats [26][27][28] . Recent studies suggest that Scutellariae Radix may have a pharmacological effect against murine colitis [10][11][12] and therefore in this study we aim to further evaluate the therapeutic effect of SRE on DSS-induced rat colitis.…”

Chinese Herbal Medicines in the Treatment of IBD and Colorectal Cancer: A Review

“…)Improvement of colitis/abolished inflammationImmune-suppressing (reduction of TNFα, IL-12, -17), wound-healing[16]W.chinensis water extractDSS-induced colitisNo datap.o.Improvement of colitis/abolished inflammationImmune-suppressing (suppression of Th1 and Th17 cells)[17]Changtai granulesChronic TNBS-induced colitisDXi.c.Improvement of colitis/abolished inflammationImmune-suppressing (suppression of macrophages/monocytes)[18]G. frondosa water extractTNBS-induced colitis5-ASAp.o.Reversed symptoms of colitisImmune-suppressing (reduction of TNFα, NF-κB and neutrophil influx)[21]Changlu EnemaProtein immunization induced-UCSASPi.c.Reversed inflammatory response (macro- and microscopically)No data[22]Mixed herbal preparationDNCB and acetic acid-induced colitisSASPi.c.Improvement of colitis/abolished inflammationImmune-suppressing (reduction of TNFα, INFγ and IL-6)[23]β,β-demethylacrylshikoninHCT-116 tumor xenograftNonei.p.Reduction of tumor growthinducing apoptosis of tumor cells (increasing level of Bax/ decreasing level of Bcl-2)[41]Shikunshito-KamihoDMH-induced CRCNonein diet (p.o. )Reduction of aberrant crypt fociNo data[42]SophoridineSW480 tumor xenograft5-FUi.p.Reduction of tumor growthinducing apoptosis of tumor cells ( activation of caspase-3 dependent pathways)[45]Spica Prunellae ethanol extractHT-29 tumor xenograftNonep.oReduction of tumor growthinduction apoptosis of tumor cells/inhibition of tumor angiogenesis and proliferation[46]

5-ASA 5-aminosalicylic acid, 5-FU 5-fluorouracil, 6-MP 6-mercaptopurine, CRC colorectal cancer, DMH 1,2-dimethylhydrazine, DNCB 2,4-dinitrochlorobenzene, DS dextran sodium sulfate, DX dexamethasone, i.c.

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