Effect of trans-resveratrol on the thrombogenicity and atherogenicity in apolipoprotein E-deficient and low-density lipoprotein receptor-deficient mice

Abstract

Resveratrol is one of the major polyphenolics in red wine that has been shown to exert the preventive effects against cardiovascular diseases. The effect of trans-resveratrol (t-RES) administered as an ingredient of the diet on the atherothrombotic tendency was assessed in genetically hypercholesterolemic mice after laser-induced damage on endothelium. Mice lacking both apolipoprotein E and low-density lipoprotein receptor (apoE-/-/LDLR-/-) were fed with a high-fat diet with or without t-RES (9.6 and 96 mg/kg diet) for 8 weeks. The atherosclerotic tendency was morphometrically analyzed in their aortae. The thrombotic tendency was determined by inducing thrombus by the irradiation of a helium-neon laser on carotid arteries of these mice with injection of Evans blue. Atherosclerotic area and thrombus size were evaluated by image analyzing in a computer system. Even though the plasma concentrations of lipids (total cholesterol and triacylglycerol) did not change in the control and t-RES groups, a significant decrease (approximately 30%) in the formation of atheroma was observed in the aortae of the t-RES group. The size of laser-induced thrombus that mostly consisted of platelet aggregates was significantly reduced (approximately 25%) in the t-RES group compared with that in the control group. Thus, t-RES orally administrated with a high-fat diet in apoE-/-/LDLR-/- mice significantly suppressed atherosclerosis in their aortae and reduced the laser-induced thrombosis in their carotid arteries.

Resveratrol attenuates angiotensin II-induced interleukin-6 expression and perivascular fibrosis

“…Although a controversy still exists in terms of the role of IL-6 in atherogenesis, resveratrol itself was reported to attenuate atherogenesis in apolipoprotein E knockout mice without a significant change in the blood lipid profile, 32 which may indicate the therapeutic or preventive implication of resveratrol in atherosclerotic cardiovascular diseases. Our study suggests that this atheroprotective effect of resveratrol may involve the inhibition of the Ang II function and the resultant suppression of IL-6 production.…”

Resveratrol: A multitargeted agent for age-associated chronic diseases

“…It was found that resveratrol reduced the atherosclerotic area and the size of the thrombus generated by the laser. 113 The possible mechanism is the inhibition of COX-1 and COX-2 by resveratrol. The synthesis of eicasoinds and leukotrienes from arcahidonic acid is also linked to platelet aggregation.…”

Health Stimulating Properties of Resveratrol

“…Fukao i wsp. [13] przeprowadzili badania z udziałem myszy apo E -/ -LDL (z wyciszonym genem odpowiedzialnym za produkcję apolipoproteiny E i receptora, liporotein o małej gęstości). Przez 8 tygodni podawano tym zwierzętom dietę eksperymentalną wysokotłuszczową z dodatkiem resweratrolu w ilości 9,6 mg/kg m.c.…”

“…spowodował znaczące zmniejszenie nasilenia procesu zapalnego, nacieku neutrofilowego i aktywności cytokin [23]. Działając razem z kwercetyną, resweratrol blokował uwalnianie z ludzkich komórek nabłonka oddechowego mediatorów stanu zapalnego: interleukiny 8 (Il-8), która "przyciąga" komórki odpornościowe oraz czynnika stymulujące-go kolonie granulocytów i makrofagów (GM-CSF) [10,13].Naukowcy zbadali również wpływ resweratrolu na wirusy. Stwierdzono, że następstwem oddziaływania tego związku jest hamowanie replikacji wirusa cytomegalii (CMV) [12].…”