Effect of tween 80 on nanoparticle preparation of modified chitosan for targeted delivery of combination doxorubicin and curcumin analogue

Sukmawati, Anita; Utami, Wahyu; Yuliani, Ratna; Da'i, Muhammad; Nafarin, Akhmad

doi:10.1088/1757-899x/311/1/012024

Cited by 24 publications

(13 citation statements)

References 14 publications

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“…The synthesis of chitosan-decorated with folate (chitosan-FA) polymer was prepared using the method described. The conjugation of chitosan-FA had confirmed using UV absorption spectra as reported in Sukmawati et al 12 The chitosan-FA showed the two high-intensity absorbances (λ 216 nm and 282 nm) compared to the chitosan polymer without modification. The absorbance peaks related to the shifting of n to π* and π to π* due to conjugation reaction between chitosan and folic acid as well as C=C bond transition.…”

Section: Resultssupporting

confidence: 57%

“…18 However, the size of the NP was influenced by the amount of tween 80 added during NP preparation. 12,19 The chitosan-FA NP had positive charge related to the amine group in chitosan (Table ). The positive charge of the NP would give the great influence for interacting with the cell as the cell surface has a negative charge.…”

Section: Characterization Of Chitosan-fa Nanoparticlementioning

confidence: 99%

“…3,6 We successfully prepared and characterized the DOX and PGV-1 loaded into chitosan-FA NP in our previous research. 12 Thus, the cytotoxic activity of the chitosan-FA in MCF-7 breast cancer cell line needs to be evaluated. The cytotoxic activity of the NP then evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Cytotoxic Evaluation of Doxorubicin and Curcumin Analogue Loaded Modified Chitosan Nanoparticles

Sukmawati¹,

Nafarin²,

Yuliani³

et al. 2018

RJC

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Chitosan had been successfully modified by conjugating with folic acid for specific delivery for an anticancer agent. The modified chitosan-folate (chitosan-FA) then prepared into a nanosize particle (NP) and loaded with the active substances, a combination of doxorubicin (DOX) and curcumin analog, 2,5-bis-(4-hydroxy,3,5-dimethyl benzylidene) cyclopentanone (Pentagamavunon-1, PGV-1). In this research, the cytotoxic activity of chitosan-FA NP containing the drugs against MCF-7 breast cancer cell was evaluated. The ionic gelation method was used for preparing of the chitosan-FA NP containing active substances. Physical characterization including particle size and zeta potential of the NP was analyzed using Particle Size Analyzer (PSA), whereas the encapsulation efficiency (EE) was analyzed using ultrafast liquid chromatography (UFLC). Anticancer activity of nanoparticles was carried out using the MTT method. The particle size obtained for chitosan-FA containing active substances was 111.8 ± 4.11 nm with the EE more than 75% for DOX and around 12% for PGV-1. The cytotoxic test with MTT assay showed that the DOX and PGV-1 loaded in chitosan-FA NP had anticancer activity against MCF-7 cells with IC50 value 25.8±2.55 µg/mL for DOX and 24.7±0.91 µg/mL for PGV-1 in combination.

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Section: Resultssupporting

confidence: 57%

Section: Characterization Of Chitosan-fa Nanoparticlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Cytotoxic Evaluation of Doxorubicin and Curcumin Analogue Loaded Modified Chitosan Nanoparticles

Sukmawati¹,

Nafarin²,

Yuliani³

et al. 2018

RJC

Self Cite

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“…This is might be due to steric repulsion rendered by its molecules which minimizes the aggregation of the vesicles. 9 Tween 80 might also act as stabilizing agent during vesicles formation that results in reduced surface energy causing inhibition of crystal growth and reduction in VS. 37 On the other hand, SPs formulated using Kolliphor RH40 as an EA had the largest VS. This behavior might be attributed to the branched structure and relative bulkiness of Kolliphor RH40 molecules leading to increased size of the vesicles.…”

Section: Resultsmentioning

confidence: 99%

Implementing Spanlastics for Improving the Ocular Delivery of Clotrimazole: In vitro Characterization, Ex vivo Permeability, Microbiological Assessment and In vivo Safety Study

Abdelbari¹,

El-Mancy²,

Elshafeey³

et al. 2021

IJN

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Purpose The aim of this study was to encapsulate clotrimazole (CLT), an antifungal drug with poor water solubility characteristics, into spanlastics (SPs) to provide a controlled ocular delivery of the drug. Methods Span 60 was used in the formulation of SPs with Tween 80, Pluronic F127, or Kolliphor RH40 as an edge activator (EA). The presence of EA offers more elasticity to the membrane of the vesicles which is expected to increase the corneal permeation of CLT. SPs were prepared using ethanol injection method applying 3 2 complete factorial design to study the effect of formulation variables (ratio of Span 60: EA (w/w) and type of EA) on SPs characteristics (encapsulation efficiency percent (EE%), average vesicle size (VS), polydispersity index (PDI) and zeta potential (ZP)). Design-Expert software was used to determine the optimum formulation for further investigations. Results The optimum formulation determined was S1, which contains 20 mg of Tween 80 used as an EA and 80 mg of Span 60. S1 exhibited EE% = 66.54 ± 7.57%, VS = 206.20 ± 4.95 nm, PDI = 0.39 ± 0.00 and ZP = −29.60 ± 0.99 mV. S1 showed highly elastic sphere-shaped vesicles. Furthermore, S1 displayed a sustained release profile and a higher ex vivo permeation across rabbit cornea relative to CLT suspension. Also, S1 revealed superior inhibition of Candida albicans development compared to CLT suspension applying 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction technique. Moreover, in vivo histopathological examination assured the safety of S1 after ophthalmic application in mature male albino rabbits. Conclusion Overall, the outcomes revealed the marked efficacy of SPs for ocular delivery of CLT.

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“…In addition, homogeneous distribution of propolis in the polymer matrix was observed. Experimental results showed that propolis was surrounded with polymer and interacted with the hydrophilic end (–OH group) of Tween 80 used as a stabilizing agent in nanosize in chitosan (-NH2) [ 84 , 85 ]. Although the homogeneity of the distribution did not change with increasing amounts of propolis, it was determined that the size of the particles increased.…”

Section: Methodsmentioning

confidence: 99%

Turkish Propolis and Its Nano Form Can Ameliorate the Side Effects of Cisplatin, Which Is a Widely Used Drug in the Treatment of Cancer

Seven

Karakuş

et al. 2020

Plants

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This study was performed to determine the effects of chitosan-coated nano-propolis (NP), which is synthesized via a green sonochemical method, and propolis on the side effects of cisplatin (CP), which is a widely used drug in the treatment of cancer. For this aim, 56 rats were divided into seven groups, balancing their body weights (BW). The study was designed as Control, CP (3 mg/kg BW at single dose of CP as intraperitoneal, ip), Propolis (100 mg/kg BW per day of propolis by gavage), NP-10 (10 mg/kg BW of NP per day by gavage), CP + Propolis (3 mg/kg BW of CP and 100 mg/kg BW of propolis), CP + NP-10 (3 mg/kg CP and 10 mg/kg BW of NP), and CP + NP-30 (3 mg/kg BW of CP and 30 mg/kg BW of NP). Propolis and NP (especially NP-30) were preserved via biochemical parameters, oxidative stress, and activation of apoptotic pathways (anti-apoptotic protein: Bcl-2 and pro-apoptotic protein: Bax) in liver and kidney tissues in the toxicity induced by CP. The NP were more effective than propolis at a dose of 30 mg/kg BW and had the potential to ameliorate CP’s negative effects while overcoming serious side effects such as liver and kidney damage.

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Effect of tween 80 on nanoparticle preparation of modified chitosan for targeted delivery of combination doxorubicin and curcumin analogue

Cited by 24 publications

References 14 publications

In Vitro Cytotoxic Evaluation of Doxorubicin and Curcumin Analogue Loaded Modified Chitosan Nanoparticles

In Vitro Cytotoxic Evaluation of Doxorubicin and Curcumin Analogue Loaded Modified Chitosan Nanoparticles

Implementing Spanlastics for Improving the Ocular Delivery of Clotrimazole: In vitro Characterization, Ex vivo Permeability, Microbiological Assessment and In vivo Safety Study

Turkish Propolis and Its Nano Form Can Ameliorate the Side Effects of Cisplatin, Which Is a Widely Used Drug in the Treatment of Cancer

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