Effect of ultrasound on size, morphology, stability and antioxidant activity of selenium nanoparticles dispersed by a hyperbranched polysaccharide from Lignosus rhinocerotis

Abstract

The differences between ultrasonic and non-ultrasonic approaches in synthesizing Lignosus rhinocerotis polysaccharide-selenium nanoparticles (LRP-SeNPs) were compared in terms of size, morphology, stability and antioxidant activity by UV-VIS, FT-IR, X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) with high resolution TEM. Results indicated that the SeNPs were associated with the LRP macromolecules in a physical adsorption pattern without breaking chemical bonds, and the ultrasonic treatment reduced the size of SeNPs, narrowed the size distribution as well as improved the stability. Due to the LRP compact coil structure loosed under ultrasonic cavitation, the SeNPs could be easily diffused into the LRP internal branches instead of gathering on the LRP surface and were well dispersed and eventually stabilized throughout the extended branches. After ultrasound treatment, the SeNPs had a minimum average diameter of ∼50 nm and the LRP-SeNPs could remain homogeneous and translucent for 16 days within 200 nm size. Furthermore, the ultrasound-treated LRP-SeNPs exhibited higher DPPH and ABTS radical-scavenging abilities than those untreated with ultrasound. This difference may be attributed to the reason that ultrasound can reduce the SeNPs size and increase the specific surface area, which provides sufficient active sites to react with the free radicals and suppress the oxidizing reactions. The integrated results demonstrated that ultrasound played a crucial role in the dispersion, size control, stabilization and antioxidant activity of SeNPs.

Preparation and growth‐promoting effect of selenium nanoparticles capped by polysaccharide‐protein complexes on tilapia

“…Overall, PSP-SeNPs remained highly dispersible for at least 12 months at 4°C. Their stable time was longer than values reported in the literature for other materials, which showed a stable period of 5 days to 3 months at 4°C 42,44,45 or about 1 month at room temperature. 26,27 Mechanism of formation of PSP-SeNPs Combined with the above results, the formation mechanism of SeNPs and the principal model of PSP capping SeNPs were proposed ( Fig.…”

Assessment of antioxidant, antibacterial and anti-proliferative (lung cancer cell line A549) activities of green synthesized silver nanoparticles fromDerris trifoliata

“…The as-prepared AgNP-DTa with a small size and large specific surface area could therefore provide a lot of active sites to scavenge the free radicals and inhibit the oxidation reactions. 61,62…”

Preparation and characterization of selenium nanoparticles decorated by Spirulina platensis polysaccharide

“…Combined with the particle size ( Figure 1b, d and f) and dispersion degree (Table 1- was 0.14 ± 0.05, indicating that the product particles were uniform. Cai et al (2017) reported that SeNPs modified by a hyperbranched polysaccharide from Lignosus rhinocerotis showed a minimum size of 50 nm. Thirty-six percent of the particles had a diameter <50 nm, and a particle size range of 50-100 and 100-300 nm of the particles accounted for 43% and 21%, respectively, which suggested that the (Cai et al, 2017).…”

“…Cai et al (2017) reported that SeNPs modified by a hyperbranched polysaccharide from Lignosus rhinocerotis showed a minimum size of 50 nm. Thirty-six percent of the particles had a diameter <50 nm, and a particle size range of 50-100 and 100-300 nm of the particles accounted for 43% and 21%, respectively, which suggested that the (Cai et al, 2017). In comparison with these results, it was clear that the size of SPs-SeNPs synthesized under optimal conditions was much smaller, indicating that the SeNPs were stabilized by SPs.…”