Effect of various exogenous and endogenous factors on microspore embryogenesis in Indian mustard (Brassica juncea (L.) Czern and Coss)

Prem, Deepak; Gupta, Kadambari; Agnihotri, Abha

doi:10.1079/ivp2005636

Cited by 42 publications

(34 citation statements)

References 23 publications

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“…Acquiring embryogenic competence requires the application of some type of external cue(s) leading to a stress(s) that preventing microspores from continuing their normal mode of gametophytic development and inducing sporophytic differentiation. In the genus Brassica, microspores are often induced by heat shock (Prem et al 2005), anti-microtubular (Würschum et al 2012), and mutagenic agents (Ahmadi et al 2012), stress hormones (Ahmadi et al 2014a) or polyamines (Ahmadi et al 2014b). Upon stress treatment and embryogenesis induction, many biochemical and morphological changes occur which is closely related to alterations in gene expression pattern (Tsuwamoto et al 2007;Muñoz-Amatriaín et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

et al. 2015

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The BnSERK1 and BnSERK2 are involved in the process of microspore embryogenesis induction, development, and plantlet regeneration. Little is known about regulatory role of somatic embryogenesis-related kinase (SERK) genes family in the induction of microspore embryogenesis, development and plant regeneration. In this study, the expression of two SERK genes (SERK1 and SERK2) was assessed during the microspore embryogenesis and plantlet regeneration in Brassica napus L. The BnSERK1 was severely up-regulated 1-5 days following microspore culture and its expression drastically decreased in the globular-heart and also torpedo staged microspore-derived embryos (MDEs). In addition, high levels of BnSERK1 transcript were detected in the MDE maturation phase and in the roots and shoots of the regenerated plantlets which indicates a broader role(s) of BnSERK1 in the organ formation, rather than being specific to the embryogenesis. Results of partial sequencing indicated that the BnSERK1 shares a conserved serine-threonine kinase catalytic domain and exhibited 95 % similarity with AtSERK1, CsSERK1, BrSERK1, NaSERK1, and NbSERK1. A steady increase in the expression of BnSERK2 was observed during the MDE initiation and development so that, the highest expression was noted in the MDE maturation phase i.e., late cotyledonary MDEs. Our results also indicated low amounts of BnSERK2 transcript at the onset of rhyzogenesis but significantly higher expression in the developing roots. In contrast, the BnSERK2 strongly up-regulated during the both initially and developed shoots. The BnSERK2 shares highly conserved LRR-RLK domain when compared with different species tested so that, high homology (100 %) was noticed with BrSERK2. Based on our findings, MDE formation and plantlet regeneration seem to be correlated with both BnSERK1 and BnSERK2 expression.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

et al. 2015

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“…In B. rapa , BA was also recommended in microspore culture without data (Ferrie 2003 ), and recently Takahashi et al ( 2012 ) demonstrated that BA enhanced embryo yields in all genotypes of B. rapa . Aminoethoxyvinylglycine (AVG) and CoCl 2 , inhibitors of ethylene biosynthesis, and silver nitrate (AgNO 3 ), an inhibitor of ethylene action, led to increased embryo yields in B. napus and B. juncea , respectively (Prem et al 2005 ;Leroux et al 2009 ). In addtion, Leroux et al ( 2009 ) reported that S-adenosylmethionine (SAM), an ethylene biosynthesis precursor, and ethephon, the ethylenereleasing agent, decreased embryo yields.…”

Section: Culture Mediummentioning

confidence: 99%

“…(reviewed by Palmer and Keller 1999 ). In contrast, AC gave a negative effect in some reports (Prem et al 2005 ;Takahashi et al 2012 ). In almost all reports showing positive effect, addition of AC with agarose was used, but in reports showing negative effect, AC was used without agarose.…”

Section: Culture Mediummentioning

confidence: 99%

Microspore Culture and Doubled Haploid Technology

Takahata

Takahashi

Tsuwamoto³

2013

Biotechnology of Crucifers

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Haploids and doubled haploids (DHs) produced by in vitro culture of gametophytic cells, especially male gametophyto, are of great importance to plant breeding and basic science. The routine protocol of isolated microspore culture of Brassica has been established, and used worldwide. However, recently, new protocols dealing with multiple samples have been developed. Although various factors infl uencing microspore embryogenesis, plant regeneration and diplodization have been examined, attempts to enhance these effi ciencies have continued. With the advancement of molecular genetics, many approaches from both forward-and reverse-genetics for understanding the mechanism of microspore embryogenesis have been attempted. This chapter reviews the recent progress of microspore embryogenesis of Brassica in relation to culture protocol and factors affecting microspore embryogenesis, and recent achievements in elucidating the mechanism of microspore embryogenesis.

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“…For example, more successful results are noted to be attained at 32.5 °C for 1 days in broccoli (Carlos & Dias 2001;Yuan et al 2011) 32.5 °C for 1 days in B. oleracea var. costata (Dias & Correia 2002), 30 °C for 2 days (Wan et al 2011), 32.5 °C for 2 days (Kim et al 2012), 30 °C for 6 days (Ahmadi et al 2012), and 32 °C for 2 days in B. napus (Prem et al 2012;Wen et al 2012); 30.5 °C for 2 days in B. oleracea (Winarto & Teixeira da Silva 2011), 35 °C for 1 days in B. rapa , 32.5 °C for 10-15 days in B. juncea (Prem et al 2005) and 32 ºC for 3 days in B. carinata (Abraha et al 2008). Brassica crops such as cabbage and kales widely grown in Turkey.…”

Section: Introductionmentioning

confidence: 99%

Effect of heat shock treatment on microspore embryogenesis in Brassica oleracea species

ÇIĞ¹

2016

Tarım Bilimleri Dergisi

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Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 °C and 35 °C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F 1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F 1 at 32 °C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 °C.

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Effect of various exogenous and endogenous factors on microspore embryogenesis in Indian mustard (Brassica juncea (L.) Czern and Coss)

Cited by 42 publications

References 23 publications

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

Microspore Culture and Doubled Haploid Technology

Effect of heat shock treatment on microspore embryogenesis in Brassica oleracea species

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