Effect of water stress on photosynthesis and in vitro activities of the PCR cycle enzymes in pigeonpea (Cajanus cajan L.)

Singal, H. R.; Sheoran, I. S.; Singh, Randhir

doi:10.1007/bf00032923

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…In Pusa362, the specific activity of NADP-GAPDH decreased by 12.5 % and in SBD377, a decrease of 17.4 % was observed after 5 hour of dehydration stress. Lack of any effect on the specific activities of glycolytic enzymes under water deficit stress was also reported by Steewart and Lee (1972) in mosses and by Singal et al (1985) in pigeon pea. In accord to our results, both these studies reported that out of all the enzymes investigated, NADP-GAPDH was the most sensitive enzyme to water potential.…”

Section: Glycolytic Enzymes and Genessupporting

confidence: 70%

Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

Khanna

Taxak

Jain

et al. 2015

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The effect of dehydration on the specific activities and transcript levels of several glycolytic enzymes was determined in two chickpea (Cicer arietinum L.) cultivars- Pusa362 (drought tolerant) and SBD377 (drought sensitive). Dehydration treatment was imposed on 6 days old chickpea seedlings by removing them from pots and keeping them on table top on 3MM Whatman paper at room temperature for 5 h. The stress imposed was judged by determining relative water content (RWC) and proline content. RWC decreased and proline content accumulated after five hours of dehydration treatment. The decline in RWC was higher in SBD377 than in Pusa362, while varietal differences were absent in proline content. With the exception of NADP-glyceraldehyde-3-phospahte dehydrogense, whose activity as well as transcript level showed marginal decrease in both the varieties, dehydration had little effect on all glycolytic enzymes studied.

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Section: Glycolytic Enzymes and Genessupporting

confidence: 70%

Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

Khanna

Taxak

Jain

et al. 2015

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“…RuBP carboxylase, 3-PGA kinase, NADP and NAD-glyceraldehyde-3-P dehydrogenase, aldolase and FBPase were extracted and assayed by standard methods reported earlier (Singal et al, 1985). In preliminary studies, the recovery, stability and optimum assay conditions for all the enzymes were established.…”

Section: Determination Of Enzyme Activitiesmentioning

confidence: 99%

Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajanus cajan L.)

1990

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The effect of Cd(2+) and Ni(2+) on the rate of photosynthesis and activities of key enzymes of the photosynthetic carbon reduction cycle was examined in leaves from pigeonpea (Cajanus cajan L., cv. UPAS-120) grown in nitrogen free sand culture. Two different concentrations of Cd(2+) and Ni(2+) were applied through the rooting medium at two growth stages. The application of Cd(2+) and Ni(2+) (0.5 and 1.0 mM) at an early vegetative stage (30 days after sowing) resulted in about 50% and 32% reduction in net photosynthesis, respectively. However, enzyme activities were decreased to different levels (2-61%) depending upon the enzyme and the concentration of the metal ion.These concentrations (0.5 and 1.0 mM of Cd(2+) and Ni(2+)) had no effect when applied at a later vegetative stage i.e. 70 days after sowing. However, when the concentration of Cd(2+) was increased to 10 mM, there was about an 86% reduction in the rate of photosynthesis but the enzyme activities were reduced by only about 40%. Although Ni(2+) reduced the photosynthetic rate by 65%, it had little effect on enzyme activities. The reduction in photosynthesis seems to occur indirectly through a decrease in chlorophyll content and stomatal conductance but not due to decreased enzyme activities. Oxygen evolution by leaf discs was inhibited by Cd(2+) and Ni(2+) in parallel with a reduction in photosynthesis. These data confirm the earlier reported effects of Cd(2+) and Ni(2+) on O2 evolution in isolated chloroplasts.

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“…Five hundred mg of tissue was taken and the enzyme extract prepared in 50mM imidazole-HC1 buffer (pH8.0) containing 10mM DTT, 10mM MgC12, 2mMEDTA and 1% (w/v) PVP as described previously [22].…”

Section: Determination Of Enzyme Activitiesmentioning

confidence: 99%

“…All the enzymes from each replicate were assayed in duplicate and the average values are reported here. The activities of RuBP carboxylase (EC 4.1.1.39), NADP-glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13), NAD-glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) and ribulose-5-phosphate kinase (EC 2.7.1.19) were determined as described previously [22]. PEP carboxylase (EC 4.1.1.31) was assayed by following the method of Hatch [8].…”

Section: Determination Of Enzyme Activitiesmentioning

confidence: 99%

In vitro enzyme activities and products of 14CO2 assimilation in flag leaf and ear parts of wheat (Triticum aestivum L.)

1986

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Activities of key enzymes of Calvin cycle and C4 metabolism, rate of (14)CO2 fixation in light and dark and the initial products of photosynthetic (14)CO2 fixation were determined in flag leaf and different ear parts of wheat viz. pericarp, awn and glumes. Compared to the activities of RuBP carboxylase and other Calvin cycle enzymes viz. NADP-glyceraldehyde-3-phosphate dehydrogenase, NAD-glyceraldehyde-3-phosphate dehydrogenase and ribulose-5-phosphate kinase, the levels of PEP carboxylase and other enzymes of C4 metabolism viz. NADP-malate dehydrogenase, NAD-malate dehydrogenase, NADP-malic enzyme, NAD-malic enzyme, glutamate oxaloacetate transaminase genase, NADP-malic enzyme, NAD-malic enzyme, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase, were generally greater in ear parts than in the flag leaf. In contrast to CO2 fixation in light, the various ear parts incorporated CO2 in darkness at much higher rates than flag leaf. In short term assimilation of (14)CO2 by illuminated ear parts, most of the (14)C was in malate with less in 3-phosphoglyceric acid, whereas flag leaves incorporated most into 3-phosphoglyceric acid. It seems likely that ear parts have the capability of assimilating CO2 by the C4 pathway of photosynthesis and utilise PEP carboxylase for recapturing the respired CO2.

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Effect of water stress on photosynthesis and in vitro activities of the PCR cycle enzymes in pigeonpea (Cajanus cajan L.)

Cited by 14 publications

References 26 publications

Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajanus cajan L.)

In vitro enzyme activities and products of 14CO2 assimilation in flag leaf and ear parts of wheat (Triticum aestivum L.)

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