Effect of wheat grass juice (triticum aestivum l.) against the erythrocytes and hemoglobin in male mice (mus musculus l.) anemia induced by sodium nitrite

Abstract

Anemia is one of the most common health problems found in Indonesia. Anemia is characterized by the low number of erythrocytes and hemoglobin in the blood caused by low levels of iron in the body. Wheat grass (Triticum aestivum L.) contain high existence of iron is supposed to be able to increase erythrocytes and Hb level in mice (Mus musculus L.). Completely Randomized Design was used in this experiment, consisting of 5 treatment (K1: without NaNO2 and WGJ (wheat grass juice), K2: given the NaNO2 without given the WGJ, P1: WGJ 25%, P2: WGJ 50%, and P3: WGJ 75%) and 5 replication. The parameters observed were the male mice erythrocytes and the Hb levels before and after being given the wheat grass juice. The data were analyzed using ANOVA then continued DNMRT test with a 5% level. The results showed that giving wheat grass juice for 14 days can increased the eryhrocytes and Hb levels in male mice. The most noticeable impact is the treatment with the highest dose i.e. P3 (WGJ 75%).

Influence of exopolysaccharide EPSKar1–iron complexation on iron bioavailability and alleviating iron deficiency anaemia in Wistar rats

“…These results are in line with the earlier studies demonstrating a significant decrease ( p < 0.05) in transferrin saturation in anaemic rats (0.82 ± 0.12 mg mL −1 ) when compared with FeSO 4 (1.98 ± 0.25 mg mL −1 ) treated rats and iron oxide nanoparticles (2.82 ± 0.99 mg mL −1 ) treated rats. 48 Likewise, our results align well with previous studies 23 reporting an increase in the serum transferrin levels in anaemic rats after administration with iron supplements along with milk lyophilates. According to a study by Gandhi et al , 38 the serum ferritin level in animals fed with only FeSO 4 was significantly lower (38.14 ± 0.43 mg mL −1 ) when compared with animals fed with the whey protein–iron complex (70.91 ± 3.87 mg mL −1 ).…”

“…It is worth mentioning that the induction of anaemia did not affect the body weight of any rat. 23 The feed and water intake was also measured daily, and the average intake was expressed on a weekly basis. During the study period, each animal consumed approximately 14.38 ± 0.92 mL of water and 13.45 ± 2.05 g of feed per day, respectively.…”

“…To monitor the blood haemoglobin levels, blood samples were collected from each animal at the 0 th , 21 st , 43 rd and 62 nd days via a retro-orbital sinus, and haemoglobin levels were assayed as described elsewhere. 23 Once the animals developed anaemia, they were transferred into metabolic cages with ad libitum access to an iron-free diet and deionised water and were daily exposed to different experimental treatments for 20 days (experimental phase) (Fig. 1).…”

Effect of Planting Media and Different Concentration of Wheat Grass Juice (Triticum aestivum L.) on Anemic Male Mice (Mus musculus L.) Leukocytes

“…Based on the summary of the DMRT test results in Table 3, it can be seen that there were significant differences in each treatment of wheat grass juice on the decrease in the number of male leukocytes in anemia, the highest decrease in the number of male leukocytes in P3 treatment with 100% concentration of wheat grass juice. According [9], research on giving wheat grass juice with a concentration of 75% can increase hemoglobin. This decrease in leukocyte count is due to the chlorophyll content found in wheat grass.…”

“…Although the soil planting media and fuel husk (hydroponics) of wheat grass have no effect on the number of leukocytes of male mice but, both planting media have their respective advantages, soil planting media are well used without providing additional nutrients and are more economical because they can use existing humus around the environment while for hydroponics using AB mix nutrients make the plant more hygienic because it is not directly contaminated by the dirt contained in the soil.amounts. Contrariwise, Nisa's research (9) shows that the ability of endophytic bacteria to produce antimicrobial compounds is lower than that of plant hosts. This difference in results can be caused by several factors, one of them is the use of solvents to extract active compounds which are not appropriate.…”