Effect of Xiao-Ai-Ping injection on paclitaxel pharmacokinetics in rats by LC–MS/MS method

Shi, Mei-Zhi; Xing, Ting-Yu; Chen, Junjun; Jiang, Bo; Xiao, Xiao; Jiao, Yang; Zhu, Jiang; Guo, Cheng; Hu, Jialei; Han, Yonglong

doi:10.1016/j.jpba.2019.07.003

Cited by 19 publications

(7 citation statements)

References 12 publications

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“…24 Our previous pharmacokinetic study has shown that XAP increased the exposure to PTX in rats. 16 Further study should be performed to evaluate the efficacy of the combination therapy in improving results. To address this, the present study aimed to refine XAP improvement of antitumor efficacy of PTX.…”

Section: Discussionmentioning

confidence: 99%

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Xiao-Ai-Ping Injection Enhances Effect of Paclitaxel to Suppress Breast Cancer Proliferation and Metastasis via Activating Transcription Factor 3

et al. 2020

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Chemotherapy is an effective treatment for invasive breast cancer. Paradoxically, many recently published findings showed that the first-line chemotherapeutic agent paclitaxel (PTX) showed pro-metastatic effects in the progress of treating breast cancer. Xiao-Ai-Ping (XAP) injection, composed of a traditional herbal medicine, Marsdenia tenacissimae extract, is known to exert antitumor effects on various cancers. However, there are few experimental studies on breast cancer. The underlying mechanism of the antitumor effect of XAP combined with chemotherapy agents has not been fully understood. In the present study, we sought to find the antitumor effects of XAP combined with PTX in vitro and in vivo. The data demonstrated that the combination of XAP with PTX resulted in remarkable enhancement of the pro-apoptotic, migration-inhibiting, and anti-invasive effects of PTX in vitro. Significantly, further study showed the overexpression of ATF3 in PTX-treated cell, while XAP counteracted the change of ATF3 induced by PTX. Moreover, it showed that combination treatment could promote the inhibition of tumor growth in MDA-MB-231 cell xenograft mouse model. Compared with PTX treatment, the downregulation of ATF3 indicated that ATF3 played a pivotal role in the combination of XAP with PTX to exert a synergistic effect. Overall, it is expected that PTX combined with XAP may serve as an effective agent for antitumor treatment, and dampening ATF3 maybe a potential strategy to improve the efficacy of PTX.

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Section: Discussionmentioning

confidence: 99%

“…[13][14][15] Our previous study had shown that XAP increased the exposure to PTX in rats. 16 However, there are few experimental studies on breast cancer, and the underlying mechanism of the antitumor efficacy of XAP combined with chemotherapy agents has not been fully understood.…”

Section: Introductionmentioning

confidence: 99%

Xiao-Ai-Ping Injection Enhances Effect of Paclitaxel to Suppress Breast Cancer Proliferation and Metastasis via Activating Transcription Factor 3

et al. 2020

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“…Although herbal medicines may improve the efficacy or reduce the side effects of conventional drugs when taken together, they also have the potential to increase the risk of clinically relevant herb−drug interactions, resulting in increased drug toxicity or even death. 10, 19, 20 Given that the use of herbal medicines is based on tradition and experience, there are no comprehensive federal regulations regarding their safety. Hence, regulatory authorities do not require rigorous preclinical and clinical evaluations of herbal medicines.…”

Section: Discussionmentioning

confidence: 99%

Effects of Palmijihwang-hwan: A Herbal Formula on the Pharmacokinetics of Tamsulosin in Rats

Jin

Jeon

Hwang

et al. 2023

Pharmacognosy Magazine

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Background Tamsulosin is an α1-adrenoceptor antagonist used to treat lower urinary tract symptoms (LUTS) and benign prostatic hyperplasia (BPH). Palmijihwang-hwan (PMJHH) is a traditional herbal formula for treating prostatic disorders and is often used with tamsulosin for treating LUTS/BPH. The co-administration of herbal medicines and conventional drugs increases the potential for clinically considerable herb−drug interactions. Hence, it is important to evaluate the safety of such combinations of drugs. This study aimed to investigate the influence of PMJHH on the pharmacokinetics of tamsulosin in rats. Materials and Methods We developed and validated a simple and rapid method to determine the plasma levels of tamsulosin in rats using an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) system. After oral administration of tamsulosin (2 mg/kg), either alone or in combination with PMJHH (930 mg/kg), to rats, blood samples were collected at various times and used for pharmacokinetic studies. The pharmacokinetic parameters were analyzed using a non-compartmental model. Results Compared to tamsulosin alone, co-administration of PMJHH had no significant effects on any pharmacokinetic parameters of tamsulosin, including maximal plasma concentration (Cmax), time to Cmax (Tmax), terminal elimination half-life ( t1/2), AUC from time 0 to the last observation (AUC0−t), mean residence from time 0 to infinity (MRT0−∞), apparent oral clearance (Cl/F), and apparent volume of distribution during terminal elimination (Vz/F). Conclusion The pharmacokinetics of tamsulosin were not affected by the co-administration of PMJHH. Our findings, therefore, validate the safety of the combination of tamsulosin and PMJHH commonly used for treating LUTS/BPH.

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“…Many chemotherapeutic agents, including PTX, are common substrates of P-gp and MRPs (Dinic et al, 2015), and the overexpression of P-gp or MRPs in tumor cells can reduce drug accumulation in cells. Thus, combining P-gp inhibitors or competing substrates of MRPs may enhance the distribution of chemotherapeutic agents and sensitize the pharmacological effects of PTX (Shi et al, 2019;Feng et al, 2020;Liu et al, 2020). JG has been commonly used to treat cough and other lung diseases clinically and is also a meridian guide drug.…”

Section: Discussionmentioning

confidence: 99%

Jiegeng Decoction Potentiates the Anticancer Efficacy of Paclitaxel in vivo and in vitro

Chen

Liu

et al. 2022

Front. Pharmacol.

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Paclitaxel (PTX) has been the first-line treatment for lung cancer; however, its clinical use is limited due to multidrug resistance (MDR) and adverse effects. Thus, there is an urgent need to explore agents that can enhance the anticancer efficacy of PTX by reducing drug resistance and adverse reactions. Jiegeng decoction (JG) was used as the meridian guide drug and adjuvant drug in treatment of lung cancer. However, the mechanism of adjuvant effect was unclear. The aim of this study was to determine whether JG could potentiate the anticancer effect of PTX. Tissue distribution of PTX was detected using HPLC-MS/MS. The anti-lung cancer effect of the combination of PTX and JG in Lewis lung cancer C57BL/6J mice was evaluated based on the body weight and tumor-inhibition rate. PTX concentration in tumors was determined using HPLC-MS and in vivo imaging. Biochemical indices were detected using biochemical analyzer and ELISA. The anticancer mechanism of the PTX-JG combination in A549/PTX cells was elucidated based on cell proliferation, annexin V-FITC apoptosis assay, and western blotting. Tissue distribution analysis showed that the distribution of PTX increased in the lungs, liver, and heart upon administering the combination of PTX and JG. JG remarkably enhanced the anticancer effect of PTX by increasing the red blood cell and platelet counts; increasing hemoglobin, interleukin (IL)-2, and tumor necrosis factor-α levels; increasing CD4+T cells and the CD4+/CD8+ ratio; and decreasing IL-10 levels. JG administration led to the increased distribution of PTX at the tumor lesion sites and also potentiated the anticancer effect of PTX by inhibiting tumor cell proliferation and promoting apoptosis. Moreover, JG reversed PTX resistance by inhibiting the expression of lung resistance-related proteins, multiresistance protein 1, P-glycoprotein, and breast cancer-resistant protein. Furthermore, the combination of JG and PTX decreased alanine aminotransferase and aspartate aminotransferase levels and did not affect creatine kinase-MB levels. Therefore, our discovery suggests that JG increased the anticancer effect of PTX by downregulating the MDR-related protein and demonstrated a synergistic enhancement of immunity. Thus, the combination of PTX with JG shows potential in the management of lung cancer owing to its synergistic and detoxifying effects.

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Effect of Xiao-Ai-Ping injection on paclitaxel pharmacokinetics in rats by LC–MS/MS method

Cited by 19 publications

References 12 publications

Xiao-Ai-Ping Injection Enhances Effect of Paclitaxel to Suppress Breast Cancer Proliferation and Metastasis via Activating Transcription Factor 3

Xiao-Ai-Ping Injection Enhances Effect of Paclitaxel to Suppress Breast Cancer Proliferation and Metastasis via Activating Transcription Factor 3

Effects of Palmijihwang-hwan: A Herbal Formula on the Pharmacokinetics of Tamsulosin in Rats

Jiegeng Decoction Potentiates the Anticancer Efficacy of Paclitaxel in vivo and in vitro

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