Effect of γ-Heptalactone on the Morphology and Production of Monascus Pigments and Monacolin K in Monascus purpureus

Shi, Ruoyu; Luo, Qiaoqiao; Liu, Yutong; Chen, Wei; Wang, Chengtao

doi:10.3390/jof8020179

Cited by 10 publications

(9 citation statements)

References 78 publications

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“…Monascus pigments and Monacolin K, the best‐known metabolites produced by Monascus , are widely used in food and medicines (Chaudhary et al, 2021), and numerous studies have focused on increasing the production of these valuable metabolites (He et al, 2021). The addition of various substances, such as tyrosol, farnesol, linoleic acid, rutin, La 3+ , γ ‐butyrolactone, genistein, and ascorbic acid, has been reported to enhance the production of Monascus pigments and Monacolin K (Erkaya et al, 2020; J. Huang et al, 2018; Z. Huang et al, 2019; H. Liu et al, 2021; Ouyang et al, 2021; Shi et al, 2022; Wei et al, 2022). Further investigation revealed that most of these exogenous molecules affect the secondary metabolism of Monascus and change the morphology of Monascus spores and mycelia by regulating relevant genes.…”

Section: Discussionmentioning

confidence: 99%

“…Further investigation revealed that most of these exogenous molecules affect the secondary metabolism of Monascus and change the morphology of Monascus spores and mycelia by regulating relevant genes. Some of these inductive molecules are quorum‐sensing molecules that play various roles in the development and metabolism of Monascus (Erkaya et al, 2020; Z. R. Huang et al, 2018; Shi et al, 2022). Quorum‐sensing molecules are related to cell density and regulate morphology and secondary metabolite production in a variety of filamentous fungi (Palonen et al, 2017a, 2017b; Raina et al, 2010; Sorrentino et al, 2010; Wang et al, 2018; Williams et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Huang et al, 2019;H. Liu et al, 2021;Ouyang et al, 2021;Shi et al, 2022;Wei et al, 2022). Further investigation revealed that most of these exogenous molecules affect the secondary metabolism of Monascus and change the morphology of Monascus spores and mycelia by regulating relevant genes.…”

Section: Effects Of Exogenous Linoleic Acid On Monascusmentioning

confidence: 99%

“…Some of these inductive molecules are quorum-sensing molecules that play various roles in the development and metabolism of Monascus (Erkaya et al, 2020;Z. R. Huang et al, 2018;Shi et al, 2022).…”

Section: Effects Of Exogenous Linoleic Acid On Monascusmentioning

confidence: 99%

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Linoleic acid functions as a quorum‐sensing molecule in Monascus purpureus–Saccharomyces cerevisiae co‐culture

Shi

Gong

Liu

et al. 2022

Yeast

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When Monascus purpureus was co-cultured with Saccharomyces cerevisiae, we noted significant changes in the secondary metabolism and morphological development of Monascus. In yeast co-culture, although the pH was not different from that of a control, the Monascus mycelial biomass increased during fermentation, and the Monacolin K yield was significantly enhanced (up to 58.87% higher). However, pigment production did not increase. Co-culture with S. cerevisiae significantly increased the expression levels of genes related to Monacolin K production (mokA-mokI), especially mokE, mokF, and mokG. Linoleic acid, that has been implicated in playing a regulating role in the secondary metabolism and morphology of Monascus, was hypothesized to be the effector. Linoleic acid was detected in the co-culture, and its levels changed during fermentation. Addition of linoleic acid increased Monacolin K production and caused similar morphological changes in Monascus spores and mycelia. Exogenous linoleic acid also significantly upregulated the transcription levels of all nine genes involved in the biosynthesis of Monacolin K (up to 69.50% higher), consistent with the enhanced Monacolin K yield.Taken together, our results showed the effect of S. cerevisiae co-culture on M. purpureus and suggested linoleic acid as a specific quorum-sensing molecule in Saccharomyces-Monascus co-culture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Effects Of Exogenous Linoleic Acid On Monascusmentioning

confidence: 99%

Section: Effects Of Exogenous Linoleic Acid On Monascusmentioning

confidence: 99%

See 2 more Smart Citations

Linoleic acid functions as a quorum‐sensing molecule in Monascus purpureus–Saccharomyces cerevisiae co‐culture

Shi

Gong

Liu

et al. 2022

Yeast

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“…Monascus spp. are industrial fungi used for food fermentation and pharmaceutical field since they can produce many beneficial metabolites, such as edible pigments, hypolipidemic compound monacolin K, blood pressure-lowering compound γ-aminobutyric acid, and highly active enzymes including glucoamylase, amylase, and esterase [2][3][4]. Recent archeology proved that the application of Monascus strain may be traced back to the Peiligang Culture Period over 8000 years ago in China.…”

Section: Introductionmentioning

confidence: 99%

Histone deacetylase MrHos3 negatively regulates the production of citrinin and pigments in Monascus ruber

et al. 2023

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Monascus spp. can produce a variety of beneficial metabolites widely used in food and pharmaceutical industries. However, some Monascus species contain the complete gene cluster responsible for citrinin biosynthesis, which raises our concerns about the safety of their fermented products. In this study, the gene Mrhos3, encoding histone deacetylase (HDAC), was deleted to evaluate its effects on the production of mycotoxin (citrinin) and the edible pigments as well as the developmental process of Monascus ruber M7. The results showed that absence of Mrhos3 caused an enhancement of citrinin content by 105.1%, 82.4%, 111.9%, and 95.7% at the 5th, 7th, 9th, and 11th day, respectively. Furthermore, deletion of Mrhos3 increased the relative expression of citrinin biosynthetic pathway genes including pksCT, mrl1, mrl2, mrl4, mrl6, and mrl7. In addition, deletion of Mrhos3 led to an increase in total pigment content and six classic pigment components. Western blot results revealed that deletion of Mrhos3 could significantly elevate the acetylation level of H3K9, H4K12, H3K18, and total protein. This study provides an important insight into the effects of hos3 gene on the secondary metabolites production in filamentous fungi.

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COMPASS core subunits MpSet1 and MpSwd3 regulate Monascus pigments synthesis in Monascus purpureus

Wu,

Gao,

Liu

2024

J Basic Microbiol

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In eukaryotes, methylation of histone H3 at lysine 4 (H3K4me) catalyzed by the complex of proteins associated with Set1 (COMPASS) is crucial for the transcriptional regulation of genes and the development of organisms. In Monascus, the functions of COMPASS in establishing H3K4me remain unclear. This study first identified the conserved COMPASS core subunits MpSet1 and MpSwd3 in Monascus purpureus and confirmed their roles in establishing H3K4me2/3. Loss of MpSet1 and MpSwd3 resulted in slower growth and development and inhibited the formation of cleistothecia, ascospores, and conidia. The loss of these core subunits also decreased the production of extracellular and intracellular Monascus pigments (MPs) by 94.2%, 93.5%, 82.7%, and 82.5%, respectively. In addition, RNA high‐throughput sequencing and quantitative real‐time polymerase chain reaction (qRT‐PCR) showed that the loss of MpSet1 and MpSwd3 altered the expression of 2646 and 2659 genes, respectively, and repressed the transcription of MPs synthesis‐related genes. In addition, the ΔMpset1 and ΔMpswd3 strains demonstrated increased sensitivity to cell wall stress with the downregulation of chitin synthase‐coding genes. These results indicated that the COMPASS core subunits MpSet1 and MpSwd3 help establish H3K4me2/3 for growth and development, spore formation, and pigment synthesis in Monascus. These core subunits also assist in maintaining cell wall integrity.

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Effect of γ-Heptalactone on the Morphology and Production of Monascus Pigments and Monacolin K in Monascus purpureus

Cited by 10 publications

References 78 publications

Linoleic acid functions as a quorum‐sensing molecule in Monascus purpureus–Saccharomyces cerevisiae co‐culture

Linoleic acid functions as a quorum‐sensing molecule in Monascus purpureus–Saccharomyces cerevisiae co‐culture

Histone deacetylase MrHos3 negatively regulates the production of citrinin and pigments in Monascus ruber

COMPASS core subunits MpSet1 and MpSwd3 regulate Monascus pigments synthesis in Monascus purpureus

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