Effects of 0.2 T static magnetic field on human skin fibroblasts

Pacini, Stefania; Gulisano, Massimo; Peruzzi, Benedetta; Sgambati, Eleonora; Gheri, G; Bryk, S. Gheri; Vannucchi, Simonetta; Polli, G.; Ruggiero, Marco

doi:10.1016/s0361-090x(03)00124-7

Cited by 44 publications

(27 citation statements)

References 12 publications

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“…Although studies have reported that magnetic field applications have decreased voltage-sensitive channel activation in GH3 cells (36), increased second messenger levels in human skin fibroblasts (30) and FNC-B4 neuronal cells (31), and increased microvessel dilation mediated by NO signaling (23), the results are mostly confounding, since they are from different cell types in vitro and tissues in vivo with varying magnetic field dosages and durations and have been applied both locally and globally, thus complicating the assessment of therapeutic value. By locally applying the SMF in conjunction with pharmacological alteration of endothelium-dependent production of NO or the Ca 2ϩ -induced contractile state in smooth muscle cells, we can begin to assess the mechanism(s) of SMF action and determine the validity of our hypotheses that SMF application might modulate the permeability and/or dilation resulting from histamine-induced edema.…”

Section: Discussionmentioning

confidence: 99%

“…Other work has suggested that SMF application may act via influence on Ca 2ϩ dynamics and NOS to regulate blood pressure and local microvascular tone (28,29). Studies have also recorded an effect of SMF on Ca 2ϩ dynamics in other, nonvascular cell types and systems (30,31,36,37,39), suggesting that application of a SMF may impact edema formation by influencing the Ca 2ϩ signal directly or indirectly through the NO signaling cascade, resulting in modulated dilation and permeability.…”

Section: Smf Application For 2 H Reduces Ca-induced Edemamentioning

confidence: 98%

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Acute exposure to a moderate strength static magnetic field reduces edema formation in rats

Morris¹,

Skalak²

2008

American Journal of Physiology-Heart and Circulatory Physiology

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Section: Discussionmentioning

confidence: 99%

Section: Smf Application For 2 H Reduces Ca-induced Edemamentioning

confidence: 98%

Acute exposure to a moderate strength static magnetic field reduces edema formation in rats

Morris¹,

Skalak²

2008

American Journal of Physiology-Heart and Circulatory Physiology

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“…Human skin fibroblast cell morphology was modified with a concomitant decrease in the expression of some sugar residues of glycoconjugates after 1h exposure to a 0.2 T static magnetic field [13]. However, cell viability, assessed by the colonyforming assay, was unaffected.…”

Section: Morphologymentioning

confidence: 99%

The review of cellular effects of a static magnetic field

Miyakoshi

2006

Science and Technology of Advanced Materials

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The effects of static magnetic fields at the cellular level are reviewed. Past studies have shown that a static magnetic field alone does not have a lethal effect on the basic properties of cell growth and survival under normal culture conditions, regardless of its magnetic density. It has also been shown that cell cycle distribution is not influenced by extremely strong static magnetic fields (up to a maximum of 10 tesla (T)). A further area of interest is whether static magnetic fields cause DNA damage, which can be evaluated by determination of the frequency of micronucleus formation. The presence or absence of such micronuclei can confirm whether a particular treatment damages cellular DNA. This method has been used to confirm that a static magnetic field alone has no such effect. However, the frequency of micronucleus formation changes significantly when certain treatments (for example, X-irradiation and mitomycin C) are given during exposure to a strong static magnetic field. It has also been reported that treatment with trace amounts of ferrous ions in the cell culture medium and exposure to a static magnetic field increases DNA damage, which is detected using the comet assay. Several reports suggest that a strong static magnetic field may affect the ion transport and the gene expression. In addition, many studies have found a strong magnetic field can induce orientation phenomena in cell culture. q

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“…The report published by World Health Organization (WHO) in 2006 gives a fair comprehension of the literature. Pacini et al [2003] exposed human skin fibroblasts to 0.2 T SMF of an MRI scanner. They studied cell morphology after 1 h exposure and found a decrease of thymidine incorporation and second messenger formation.…”

Section: Introductionmentioning

confidence: 99%

Effects of homogeneous and inhomogeneous static magnetic fields combined with gamma radiation on DNA and DNA repair

Kubinyi

Zeitler

Thuróczy

et al. 2010

Bioelectromagnetics

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The aim of this study was to reveal whether static magnetic fields (SMFs) influence the repair of radiation-damaged DNA on leukocytes or has any effect on DNA. After 4 Gy of (60)Co-gamma irradiation, some of the samples were exposed to inhomogeneous SMFs with a lateral magnetic flux density gradient of 47.7, 1.2, or 0.3 T/m by 10 mm lateral periodicity, while other samples were exposed to homogeneous SMF of 159.2 +/- 13.4 mT magnetic flux density for a time period of 0.5 min, 1, 2, 4, 6, 18, 20, or 24 h. Another set of samples was exposed to the aforementioned SMFs before gamma irradiation. The following three groups were examined: (i) exposed to SMF only, (ii) exposed to SMF following irradiation by (60)Co-gamma, and (iii) exposed to SMF before (60)Co-gamma irradiation. The analysis of the DNA damage was made by single-cell gel electrophoresis technique (comet assay). Statistically significant differences were found at 1 h (iSMF), 4 h (hSMF), and 18 h (hSMF) if samples were exposed to only SMF, compared to control. When the SMF exposure followed the (60)Co-gamma irradiation, statistically significant differences were found at 1 h (iSMF) and 4 h (hSMF). If exposure to SMF preceded (60)Co-gamma irradiation, no statistically significant difference was found compared to 4 Gy gamma-irradiated group.

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Effects of 0.2 T static magnetic field on human skin fibroblasts

Cited by 44 publications

References 12 publications

Acute exposure to a moderate strength static magnetic field reduces edema formation in rats

Acute exposure to a moderate strength static magnetic field reduces edema formation in rats

The review of cellular effects of a static magnetic field

Effects of homogeneous and inhomogeneous static magnetic fields combined with gamma radiation on DNA and DNA repair

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