Effects of albumin administration in serum liver enzymes of rats in the presence of extrahepatic biliary obstruction

Lessa, Jorge Fernando Rebouças; Rangel, Leonardo Siqueira; Costa, Nilton José de Almeida; Silva, Orlando Castro e; Cruz, Camilo; Sousa, João Batista de

doi:10.1590/s0102-86502011000800013

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…Similar findings were also generated by Woolbright et al[20]. In a study by Lessa et al[21] an additional increase of transaminases during the third week after BDL was visible (but not significant), which was also detectable in our data. This phenomenon might be explained by the small number of animals in each group.…”

Section: Discussionsupporting

confidence: 93%

Induction of chronic cholestasis without liver cirrhosis - Creation of an animal model

Dondorf¹,

Fahrner²,

Ardelt³

et al. 2017

WJG

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AIMTo analyze time intervals of inflammation and regeneration in a cholestatic rat liver model.METHODSIn 36 Lewis rats, divided into six groups of 6 animals (postoperative observation periods: 1, 2, 3, 4, 6, 8 wk), the main bile duct was ligated with two ligatures and observed for the periods mentioned above. For laboratory evaluation, cholestasis parameters (bilirubin, γ-GT), liver cell parameters (ASAT, ALAT) and liver synthesis parameters (quick, albumin) were determined. For histological analysis, HE, EvG, ASDCL and HMGB-1 stainings were performed. Furthermore, we used the mRNA of IL-33, GADD45a and p-21 for analyzing cellular stress and regeneration in cholestatic rats.RESULTSIn chemical laboratory and histological evaluation, a distinction between acute and chronic cholestatic liver injury with identification of inflammation and regeneration could be demonstrated by an increase in cholestasis (bilirubin: 1-wk group, 156.83 ± 34.12 μmol/L, P = 0.004) and liver cell parameters (ASAT: 2-wk group, 2.1 ± 2.19 μmol/L.s, P = 0.03; ALAT: 2-wk group, 1.03 ± 0.38 μmol/L.s, P = 0.03) after bile duct ligation (BDL). Histological evaluation showed an increase of bile ducts per portal field (3-wk group, 48 ± 6.13, P = 0.004) during the first four weeks after bile duct ligation. In addition to inflammation, which is an expression of acute cholestasis, there was an increase of necrotic areas in the histological sections (2-wk group, 1.38% ± 2.28% per slide, P = 0.002). Furthermore, the inflammation could be verified by ASDCL (4-wk group, 22 ± 5.93 positive cells per portal field, P = 0.041) and HMGB-1 [2-wk group, 13 ± 8.18 positive cells per field of view (FoV), P = 0.065] staining. Therefore, in summary of the laboratory evaluation and histological studies, acute cholestasis could be found during the first four weeks after bile duct ligation. Subsequently, the described parameters declined so that chronic cholestasis could be assumed. For quantification of secondary biliary cirrhosis, eosin staining was performed, which did not reveal any signs of liver remodeling, thus precluding the development of a chronic cholestasis model. Additionally, to establish the chronic cholestasis model, we evaluated liver regeneration capacity through measurements of IL-33, p-21 and GADD45a mRNA.CONCLUSIONWe created a chronic cholestasis model. The point of inflammatory and regenerative balance was reached after four weeks. This finding should be used for experimental approaches dealing with chronic cholestatic liver damage.

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Section: Discussionsupporting

confidence: 93%

Induction of chronic cholestasis without liver cirrhosis - Creation of an animal model

Dondorf¹,

Fahrner²,

Ardelt³

et al. 2017

WJG

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“…• Group III: BDL/untreated (n = 6) rats underwent laparotomy with ligation of the common bile duct and were sacrificed 4 weeks after the common bile duct ligation. • Group IV: BDL/treated (n = 6) rats underwent laparotomy with ligation of the common bile duct, were treated with 2% (albumin aqueous solution) ad libitum [12], and were sacrificed 4 weeks after the common bile duct ligation.…”

Section: Animal Groupsmentioning

confidence: 99%

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

Saad

Fathelbab

El-Saba

et al. 2016

Journal of Taibah University for Science

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The aim was to study the influence of albumin supplementation on the changes of the kidney function and structure in cirrhotic rats induced by common bile duct ligation (BDL). Twenty-four male albino rats weighing 200-250 g were divided into Group I: 6 rats underwent laparotomy alone, and the bile duct was only dissected from the surrounding tissue; Group II: 6 rats underwent a sham operation and received 2% albumin in their drinking water; Group III: 6 rats were subjected to bile duct ligation only; and Group IV: 6 rats were subjected to bile duct ligation and received a daily albumin 2% in drinking water. All rats were sacrificed after 4 weeks. We measured the liver and kidney functions and oxidative stress markers in the renal tissue and conducted a histological evaluation of the liver and kidney. The liver enzymes were decreased, but there was no significant difference in the bilirubin levels in group IV compared to group III. There was a significant elevation of serum creatinine in group III compared to group II, and serum creatinine was attenuated in group IV. The renal tissue catalase activity and reduced glutathione, as well as the nitric oxide levels, were significantly increased in group IV and were elevated in group III. Histologically, the livers of group IV showed degeneration and inflammatory cell infiltration with regeneration areas in which normal hepatocytes appeared. The kidneys of group IV showed recovery as well as areas of inflammatory cell infiltration. Some tubules appeared with normal epithelial lining. In conclusion, the results suggest that albumin partially improves the renal functions and structures after their disturbances as a result of BDL.

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Effects of albumin administration in serum liver enzymes of rats in the presence of extrahepatic biliary obstruction

Cited by 2 publications

References 10 publications

Induction of chronic cholestasis without liver cirrhosis - Creation of an animal model

Induction of chronic cholestasis without liver cirrhosis - Creation of an animal model

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

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