Effects of ANGPTL3 Antisense Oligodeoxynucleotides Transfection on the Cell Growths and Invasion of Human Hepatocellular Carcinoma Cells

Yu, Haibo; Zhang, Huifeng; Li, Deyu; Xue, Huanzhou; Pan, Cheng-En; Zhao, Sihai; Wang, Lei

doi:10.5754/hge10647

Cited by 26 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study of cellular proliferation mechanism in tumoral cells is important for tumoral treatment and molecular biology . ANGPTL3 has been reported to be associated with tumoral development and progression . In this study, suppression of ANGPTL3 by shRNA significantly decreased cellular proliferation by inhibiting the ERK pathway (Fig.…”

Section: Discussionmentioning

confidence: 63%

ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer

et al. 2015

View full text Add to dashboard Cite

Angiopoietin-like 3 (ANGPTL3), which is involved in new blood vessel growth and stimulation of mitogen-activated protein kinase (MAPK), is expressed aberrantly in several types of human cancers. However, little is known about the relevance of ANGPTL3 in the behavior of oral squamous cell carcinoma (OSCC). In this study, we evaluated ANGPTL3 mRNA and protein in OSCC-derived cell lines (n = 8) and primary OSCCs (n = 109) and assessed the effect of ANGPTL3 on the biology and function of OSCCs in vitro and in vivo. Significant (P < 0.05) ANGPTL3 upregulation was detected in the cell lines and most primary OSCCs (60%) compared with the normal counterparts. The ANGPTL3 expression level was correlated closely (P < 0.05) with tumoral size. In patients with T3/T4 tumors, the overall survival rate with an ANGPTL3-positive tumor was significantly (P < 0.05) lower than that of ANGPTL3-negative cases. In vitro, cellular growth in ANGPTL3 knockdown cells significantly (P < 0.05) decreased with inactivated extracellular regulated kinase (ERK) and cell-cycle arrest at the G1 phase resulting from upregulation of the cyclin-dependent kinase inhibitors, including p21Cip1 and p27Kip1. We also observed a marked (P < 0.05) reduction in the growth in ANGPTL3 knockdown-cell xenografts with decreased levels of phosphorylated ERK relative to control-cell xenografts. The current data indicated that ANGPTL3 may play a role in OSCCs via MAPK signaling cascades, making it a potentially useful diagnostic/therapeutic target for use in patients with OSCC.

show abstract

Section: Discussionmentioning

confidence: 63%

ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer

et al. 2015

View full text Add to dashboard Cite

show abstract

“…However, reports about the role of ANGPTLs in cancer were nearly blank, except for ANGPTL1 and ANGPTL4. ANGPTL3 is strongly involved in the development of cancer, for example ovarian cancer and liver cancer [9,10]. Here, we are the first to characterize the role of ANGPTL3 expression in glioblastomas.…”

Section: Discussionmentioning

confidence: 97%

“…As a secreted protein, reduced levels of ANGPTL3 have also been detected in the patients with ovarian cancer [9]. Downregulation of ANGPTL3 in hepatocellular carcinoma cells inhibits cell proliferation and invasion in vitro [10]. However, there are no published data regarding the role of ANGPTL3 in gliomas to date.…”

mentioning

confidence: 99%

Clinical significance of angiopoietin-like protein 3 expression in patients with glioblastoma

Wang¹,

Li²,

Qi³

et al. 2016

neo

View full text Add to dashboard Cite

There is strong evidence that angiopoietin-like peptide family is involved in the invasiveness and metastasis of cancer. Angiopoietin-like protein 3 (ANGPTL3) is proven to be involved in angiogenesis and tumor development. However, there is no published data on the role ANGPLT3 plays in glioblastomas. The present study was conducted to examine ANGPLT3 proteins expression and its association with clinicopathological factors and prognosis in human glioblastomas. Immunohistochemistry was used to evaluate the expression of ANGPTL3, EGFR and VEGFR. We found that 33 cases (57.9%) that showed strong immunostaining for ANGPTL3 proteins. However, there was no significant difference between the expression of ANGPTL3 and the proangiogenic factors, including EGFR or VEGFR. Patients with high/moderate expression of ANGPTL3 had a significantly shorter survival time (6.3 months) than those (median survival time 13.8 months) with low/ negative expression. The overall survival (OS) was also investigated and analyzed by the Kaplan-Meier method, which showed a significant difference (P=0.0045, Breslow test). The present data leads to new insights into the role of ANGPTL3 in glioblastomas and provides an independent predictive factor.

show abstract

“…Among these changing factors, we attributed MMP9 to exosomal ANGPTL1 dependent vascular leakiness prevention. ANGPTLs were reported to regulate the expression of MMP9 in osteosarcoma [43] and HCC [44]. And MMP9 is known to be involved in regulating vascular integrity in PMNs [22].…”

Section: Discussionmentioning

confidence: 99%

Exosomal ANGPTL1 Attenuates CRC Liver Metastasis by Regulating Kupffer Cell Secretion Pattern and Impeding MMP9 induced vascular leakiness.

Jiang

Chen

Fang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Angiopoietin-like protein 1 (ANGPTL1) has been proved to suppress tumor metastasis in several cancers. However, its extracellular effects on the pre-metastatic niches (PMNs) are still unclear. ANGPTL1 has been identified in exosomes, while its function remains unknown. This study was designed to explore the role of exosomal ANGPTL1 on liver metastasis in colorectal cancer (CRC).Methods: Exosomes were isolated by ultracentrifugation. The ANGPTL1 level was detected in exosomes derived from human CRC tissues. The effects of exosomal ANGPTL1 on CRC liver metastasis were explored by the intrasplenic injection mouse model. The liver PMN was examined by vascular permeability assays. Exosomal ANGPTL1 localization was validated by exosome labeling. The regulatory mechanisms of exosomal ANGPTL1 on Kupffer cells were determined by RNA sequencing. qRT-PCR, Western Blot, and ELISA analysis were conducted to examine gene expressions at mRNA and protein levels.Results: ANGPTL1 protein level was significantly downregulated in the exosomes derived from CRC tumors compared with paired normal tissues. Besides, exosomal ANGPTL1 attenuated liver metastasis and impeded vascular leakiness in the liver PMN. Moreover, exosomal ANGPTL1 were mainly taken up by KCs and regulated the KCs secretion pattern, especially decreasing the MMP9 expression, which finally prevented the liver vascular leakiness. In mechanism, exosomal ANGPTL1 downregulated MMP9 level in KCs by inhibiting the JAK2-STAT3 signaling pathway.Conclusions: Taken together, exosomal ANGPTL1 attenuated CRC liver metastasis and impeded vascular leakiness in the liver PMN by reprogramming the Kupffer cell and decreasing the MMP9 expression. This study suggests a suppression role of exosomal ANGPTL1 on CRC liver metastasis and expands the approach of ANGPTL1 functioning.

show abstract

Effects of ANGPTL3 Antisense Oligodeoxynucleotides Transfection on the Cell Growths and Invasion of Human Hepatocellular Carcinoma Cells

Abstract: ANGPTL3 may be functionally involved in hepatocellular carcinoma cell proliferation and invasion and is a potential target for hepatocellular carcinoma therapy.

Cited by 26 publications

References 0 publications

ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer

ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer

Clinical significance of angiopoietin-like protein 3 expression in patients with glioblastoma

Exosomal ANGPTL1 Attenuates CRC Liver Metastasis by Regulating Kupffer Cell Secretion Pattern and Impeding MMP9 induced vascular leakiness.

Contact Info

Product

Resources

About