Effects of anodic titanium dioxide nanotubes of different diameters on macrophage secretion and expression of cytokines and chemokines

Lu, Wen-Cai; Wang, N.; Gao, Ping; Li, C. Y.; Zhao, Haige; Zhang, Z. T.

doi:10.1111/cpr.12149

Cited by 54 publications

(31 citation statements)

References 43 publications

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“…At the micron level, blasting and etching produces a severely pitted surface and subsequent storage in saline not only results in a hydrophilic surface (modSLA), but also the spontaneous formation of nanoscale sized surface structures (Wennerberg, Svanborg, Berner, & Andersson, ). These features have been shown by our group (Alfarsi et al, ; Hamlet et al, ) and others (Hotchkiss et al, , ; Lu & Webster, ; Lü et al, ) to affect both the attachment of monocytes and macrophages, as well as the gene expression and secretion of pro‐inflammatory cytokines.…”

Section: Discussionsupporting

confidence: 73%

Hydrophilic titanium surface‐induced macrophage modulation promotes pro‐osteogenic signalling

Hamlet

Lee

Moon

et al. 2019

Clinical Oral Implants Res

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Objectives As biomaterial‐induced modulation of mediators of the immune response may be a potential therapeutic approach to enhance wound healing events, the aim of this study was to delineate the effects of titanium surface modification on macrophage phenotype and function. Material and methods Rodent bone marrow‐derived macrophages were polarized into M1 and M2 phenotypes and cultured on micro‐rough (SLA) and hydrophilic modified SLA (modSLA) titanium discs. Macrophage phenotype and cytokine secretion were subsequently assessed by immunostaining and ELISA, respectively. Osteoblast gene expression in response to culture in the M1 and M2 macrophage conditioned media was also evaluated over 7 days by RT‐PCR. Results M1 macrophage culture on the modSLA surface promoted an M2‐like phenotype as demonstrated by marked CD163 protein expression, Arg1 gene expression and the secretion of cytokines that significantly upregulated in osteoblasts the expression of genes associated with the TGF‐ß/BMP signalling pathway and osteogenesis. In comparison, M2 macrophage culture on SLA surface promoted an inflammatory phenotype and cytokine profile that was not conducive for osteogenic gene expression. Conclusions Macrophages are able to alter or switch their phenotype according to the signals received from the biomaterial surface. A hydrophilic micro‐rough titanium surface topography elicits a macrophage phenotype associated with reduced inflammation and enhanced pro‐osteogenic signalling.

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Section: Discussionsupporting

confidence: 73%

Hydrophilic titanium surface‐induced macrophage modulation promotes pro‐osteogenic signalling

Hamlet

Lee

Moon

et al. 2019

Clinical Oral Implants Res

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“…To date, many studies have addressed the key roles of Ti implants in anti-inflammatory responses and have emphasized the important role of nanostructured surfaces. 44,[51][52][53][54] However, most of these studies have focused on the proliferation, adhesion, and secretion of pro-inflammatory cytokines from macrophages on the material. [53][54][55] Yao et al similarly used different voltages to fabricate nanotube surfaces and found that larger nanotubular surfaces (120 nm) could inhibit the expression and secretion of pro-inflammatory cytokines in macrophages.…”

mentioning

confidence: 99%

Nanotubular TiO<sub>2</sub> regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

Dong

Ding

et al. 2019

IJN

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BackgroundMacrophages play important roles in the immune response to, and successful implantation of, biomaterials. Titanium nanotubes are considered promising heart valve stent materials owing to their effects on modulation of macrophage behavior. However, the effects of nanotube-regulated macrophages on endothelial cells, which are essential for stent endothelialization, are unknown. Therefore, in this study we evaluated the inflammatory responses of endothelial cells to titanium nanotubes prepared at different voltages.Methods and resultsIn this study we used three different voltages (20, 40, and 60 V) to produce titania nanotubes with three different diameters by anodic oxidation. The state of macrophages on the samples was assessed, and the supernatants were collected as conditioned media (CM) to stimulate human umbilical vein endothelial cells (HUVECs), with pure titanium as a control group. The results indicated that titanium dioxide (TiO2) nanotubes induced macrophage polarization toward the anti-inflammatory M2 state and increased the expression of arginase-1, mannose receptor, and interleukin 10. Further mechanistic analysis revealed that M2 macrophage polarization controlled by the TiO2 nanotube surface activated the phosphatidylinositol 3-kinase/AKT and extracellular signal-regulated kinase 1/2 pathways through release of vascular endothelial growth factor to influence endothelialization.ConclusionOur findings expanded our understanding of the complex influence of nanotubes in implants and the macrophage inflammatory response. Furthermore, CM generated from culture on the TiO2 nanotube surface may represent an integrated research model for studying the interactions of two different cell types and may be a promising approach for accelerating stent endothelialization through immunoregulation.

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“…The cellular responses are different toward one-dimensional arrays, such as implants and scaffolds with varying morphologies and structures [140][141][142]. The morphology, including pore size (or top-side diameter) and length of the tubular TiO 2 arrays, mainly determines cell viability and proliferation through the modulation of the focal adhesion kinase and Ras homolog family member A (RhoA) pathways [35,143,144]. The macrophage inflammatory effect of tubular arrays is also controllable through the inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways [145].…”

Section: In Vitro Cytotoxicity Assessmentsmentioning

confidence: 99%

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Kafshgari

Goldmann

2020

Nano-Micro Lett.

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HIGHLIGHTS • Multifunctional TiO 2 nanostructures hold promise for advancing a wide range of biomedical applications due to a feasible integration of distinct theranostic features. • Fabrication and post-fabrication strategies implemented to generate multifunctional TiO 2 nanostructures for a broad range of biomedical applications are briefly outlined. The opportunities and challenges of TiO 2 nanomaterials are highlighted in order to open the possibility of clinical translation. ABSTRACT Titanium dioxide (TiO 2 ) nanostructures exhibit a broad range of theranostic properties that make them attractive for biomedical applications. TiO 2 nanostructures promise to improve current theranostic strategies by leveraging the enhanced quantum confinement, thermal conversion, specific surface area, and surface activity. This review highlights certain important aspects of fabrication strategies, which are employed to generate multifunctional TiO 2 nanostructures, while outlining post-fabrication techniques with anemphasis on their suitability for nanomedicine. The biodistribution, toxicity, biocompatibility, cellular adhesion, and endocytosis of these nanostructures, when exposed to biological microenvironments, are examined in regard to their geometry, size, and surface chemistry. The final section focuses on recent biomedical applications of TiO 2 nanostructures, specifically evaluating therapeutic delivery, photodynamic and sonodynamic therapy, bioimaging, biosensing, tissue regeneration, as well as chronic wound healing.

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Effects of anodic titanium dioxide nanotubes of different diameters on macrophage secretion and expression of cytokines and chemokines

Abstract: TiO2 nanotube surfaces, especially of 80 nm TiO2 nanotube, reduced inflammatory response in vitro, which might be part of a basis for rapid osseointegration in implants with TiO2 nanotube surfaces in animal studies.

Cited by 54 publications

References 43 publications

Hydrophilic titanium surface‐induced macrophage modulation promotes pro‐osteogenic signalling

Hydrophilic titanium surface‐induced macrophage modulation promotes pro‐osteogenic signalling

Nanotubular TiO<sub>2</sub> regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

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