Effects of antioxidants on DNA-double strand breaks in human gingival fibroblasts exposed to methacrylate based monomers

Lottner, S.; Shehata, M. M.; Hickel, Reinhard; Reichl, Franz‐Xaver; Durner, Jürgen

doi:10.1016/j.dental.2013.07.005

Cited by 34 publications

(23 citation statements)

References 37 publications

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“…DSB formation was studied using colocalizing foci of γ-H2AX and 53BP1 as surrogate markers for DSBs, which is widely accepted in the literature 28,30,33) . As compared to an earlier study 56) that also used Bis-GMA at 0.09 mM, we noted the induction of 2.6 fpc in the γ-H2AX/53BP1 DSB focus assay, which is significantly lower compared to the 4.1 fpc observed with γ-H2AX focus staining alone 56) . This difference likely relates to the use of γ-H2AX-only-foci staining by Lottner et al In the present study only colocalizing γ-H2AX and 53BP1 foci ( Fig.…”

Section: Discussionmentioning

confidence: 57%

NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Styllou

Hickel

et al. 2017

Dental Materials Journal

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Released (co)monomers from dental composite components can induce DNA damage of which DNA double-strand breaks (DSBs) threaten genome integrity. Here, we tested whether the administration of the antioxidant N-acetylcysteine (NAC) is able to reduce the dental composite-induced DSBs in primary human gingiva fibroblasts. The dental composites Bis-GMA (bisphenol-Aglycerolate dimethacrylate), GMA (glycidyl methacrylate), HEMA (2-hydroxyethyl methacrylate) and TEGDMA (triethyleneglycol dimethacrylate) were found to induce co-localizing microscopic nuclear foci numbers of the DSB markers γ-H2AX and 53BP1 per cell in the order: GMA>Bis-GMA>TEGDMA>HEMA. Supplementation of (co)monomer-containing culture medium with NAC led to a significant reduction of resin-induced DSBs as well as to an amelioration of dental monomer-induced nuclear chromatin condensation in gingival fibroblasts. Thus, antioxidant treatment can reduce radical-induced chromatin and DNA damage and open avenues to mitigate genotoxic effects of dental composite compounds.

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Section: Discussionmentioning

confidence: 57%

NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Styllou

Hickel

et al. 2017

Dental Materials Journal

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“…These observations were also consistent with previous publication which demonstrated that the supplement of GSH could attenuate methacrylic monomer-induced genotoxicity. Lottner et al found that the addition of NAC could reduce the DSBs induced by methacrylic monomers 25 . Likewise, Schweikl et al found that the presence of NAC could reduce the formation of micronuclei caused by TEGDMA and HEMA 28 .…”

Section: Discussionmentioning

confidence: 99%

Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress

Jiao¹,

Ma²,

Wang³

et al. 2016

Int. J. Biol. Sci.

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The polymerizable antibacterial monomer methacryloxylethyl cetyl ammonium chloride (DMAE-CB) has provided an effective strategy to combat dental caries. However, the application of such material raises the question about the biological safety and the question remains open. The mechanism of this toxic action, however, is not yet clearly understood. The present study aims at providing novel insight into the possible causal link between cellular oxidative stress and DNA damage, as well as apoptosis in human dental pulp cells exposed to DMAE-CB. The enhanced formation of reactive oxygen species and depletion of glutathione, as well as differential changes in activities of superoxide dismutase, glutathione peroxidase, and catalase in DMAE-CB-treated cells indicated oxidative stress. By using substances that can alter GSH synthesis, we found that GSH was the key component in the regulation of cell response towards oxidative stress induced by DMAE-CB. The increase in oxidative stress-sensitive 8-Oxo-2'-deoxyguanosine (8-OHdG) content, formation of γ-H2AX and cell cycle G1 phase arrest indicated that DNA damage occurred as a result of the interaction between DNA base and ROS beyond the capacities of antioxidant mechanisms in cells exposed to DMAE-CB. Such oxidative DNA damage thus triggers the activation of ataxia telangiectasia-mutated (ATM) signaling, the intrinsic apoptotic pathway, and destruction of mitochondrial morphology and function.

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“…In this way, they reach the systemic circulation 36,37) , where they are further metabolized 38,39) . Studies have shown that cellular or liver microsomal degradation of (co)monomers leads to the formation of the epoxy compound 2,3-epoxymethacrylic acid 38,[40][41][42][43][44][45][46][47][48][49][50][51][52] . Composite particles may also be generated during wear processes.…”

Section: (Courtesy Van Landuyt and By Dental Materials)mentioning

confidence: 99%

Wear of dental materials: Clinical significance and laboratory wear simulation methods —A review

Heintze

Reichl²,

Hickel

2019

Dent. Mater. J.

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This review focusses on tribological aspects of teeth during function, the clinical significance of wear, wear of natural teeth and restorative materials and laboratory methods to simulate wear of restorative materials. Ceramic, metal alloy and amalgam show low material wear, whereas resin-based materials demonstrate substantial wear in the long term. The clinical wear shows a high variability with the patient factor accounts for about 50% of the variability. Wear as such seldomly compromises the function of the stomatognath system or individual teeth and is in most cases an esthetic problem. Particles that are ingested due to attrition and abrasion wear may pose a health risk to the patient, especially those from composite resin materials. However, systematic clinical studies on that issue are not available. For laboratory research many wear simulation devices and methods have been developed but only few are validated and have a moderate correlation with clinical wear.

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Effects of antioxidants on DNA-double strand breaks in human gingival fibroblasts exposed to methacrylate based monomers

Cited by 34 publications

References 37 publications

NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress

Wear of dental materials: Clinical significance and laboratory wear simulation methods —A review

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