Effects of Aquifer Heterogeneity and Geochemical Variation on Petroleum-Hydrocarbon Biodegradation at a Gasoline Spill Site

Abstract

In subsurface environment, small-scale heterogeneities usually cause the reduction of the applicability of in situ remedial techniques. Biogeochemical heterogeneities and preferential groundwater flow paths create complex hydrogeologic conditions at most contaminated sites. A thorough understanding of the resulting three-dimensional distribution of contaminants is a necessity prior to determining a need for remediation. In this study, a gasoline spill site was selected to examine the effects of aquifer heterogeneities and geochemical variations on petroleum hydrocarbon biodegradation via different oxidation-reduction process. At this site, two multilevel sampling wells were installed to delineate the lateral (5 m) and vertical (0.5 m) distribution of contaminant concentrations and different biogeochemical parameters. Two 5-cm (I.D.) continuous soil cores [from 4 to 8 m below land surface (bls)] were collected within the gasoline plume to evaluate the distribution of the microbial population in soils. Results show that high microbial activities were observed in soil samples based on the following evidences: (1) high petroleum hydrocarbon degradation rate, and (2) high microbial biomass. Each soil section was used for chemical extraction, microbial enumeration, and grain size distribution. Results show that the soil sections with more permeable sediment materials corresponded with higher biomass (total anaerobes > 2 x 106cells/g) and significant contaminant degradation. However, those sections with less permeable sediments contained lower microbial population. Results indicate that the subsurface microorganisms were distributed unevenly in the aquifer, and some regions were devoid of microorganisms and biodegradation activities. Spatial distribution of microorganisms, soil materials, and biogeochemical characteristics in the subsurface soils control the extent and kinetics of contaminant biodegradation. Thus, using blended aquifer materials for measurement of in situ biodegradation rates may not achieve representative results.

Diverse Metabolic Capacities of Fungi for Bioremediation

“…Bioremediation of pollutants can be carried out by applying any one of the following processes such as natural attenuation, biostimulation and bioaugmentation or a combination thereof. This has been aptly demonstrated during the bioremediation of atrazine [4], petroleum hydrocarbons [5,6], and tri-nitro toluene (TNT) [7,8] in soil microcosms. Though bioremediation technologies for industrial chemicals based on activated sludge microorganisms are well-established [9,10], their performance has been found to be relatively less efficient for removing persistent trace organic contaminants (TrOCs) [11,12].…”