Effects of astilbic acid on airway hyperresponsiveness and inflammation in a mouse model of allergic asthma

Yuk, Ji-Eun; Lee, Mee-Young; Kwon, Ok‐Kyoung; Cai, Xing-Fu; Jang, Ha-Young; Oh, Sei‐Ryang; Lee, Hyeong‐Kyu; Ahn, Ki Hoon

doi:10.1016/j.intimp.2010.12.002

Cited by 29 publications

(15 citation statements)

References 31 publications

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“…Taken together, these previous results provide strong evidence for a crucial role of Th2 cytokines in the pathogenesis of allergic asthma. Therefore, a decrease in Th2 cytokine secretion could exhibit therapeutic benefits to asthmatic patients and animal models Yuk et al 2011). In contrast, the AcCystatin-treated rats exhibited reduced Th2 cytokine expression, with a reduction in IL-4 and IL-5 levels.…”

Section: Discussionmentioning

confidence: 99%

AcCystatin, an immunoregulatory molecule from Angiostrongylus cantonensis, ameliorates the asthmatic response in an aluminium hydroxide/ovalbumin-induced rat model of asthma

Yang

et al. 2014

Parasitol Res

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Epidemiological surveys have demonstrated that helminth infections are negatively related to atopic diseases, including asthma. Defining and characterising specific helminth molecules that have excellent immunomodulatory capacities as potential therapeutics for the treatment or prophylaxis of allergic manifestations are of great interest. AcCystatin, a cystatin protease inhibitor of Angiostrongylus cantonensis, is a homologue of other nematode cystatins with immunoregulatory properties. Here, we aim to determine the effects of AcCystatin on an ovalbumin/aluminium hydroxide (OVA/Al[OH]3)-induced rat model of asthma. Wistar rats were randomly divided into four groups, including a control group, an OVA/Al[OH]3-induced asthma group, a group receiving AcCystatin immunisation prior to OVA/Al[OH]3-induced asthma and a group receiving AcCystatin treatment after OVA/Al[OH]3-induced asthma. The numbers of eosinophils, basophils, neutrophils, lymphocytes and monocytes in the peripheral blood and of eosinophils in the bronchoalveolar lavage fluid (BALF) were counted for each animal. The expression levels of the cytokines interferon-γ, interleukin (IL) 4, IL-5, IL-6, IL-10, IL17A and tumour necrosis factor receptor-α in BALF, of OVA-specific immunoglobulin E in BALF and serum and of the chemokines eotaxin-1, eotaxin-2, eotaxin-3, MCP-1 and MCP-3 in lung tissue were measured. In addition, the degree of peribronchial and perivascular inflammation and the intensity of goblet cell metaplasia were qualitatively evaluated. The sensitised/challenged rats developed an extensive cell inflammatory response of the airways. AcCystatin administration significantly reduced the cellular infiltrate in the perivascular and peribronchial lung tissues and reduced both goblet mucous production and eosinophil infiltration. The rats that were treated with AcCystatin before or after sensitisation with OVA showed significant decreases in eotaxin-1, eotaxin-3 and MCP-1 expression in the lung tissue. The production of IL-4, IL-5, IL-6 and IL-17A and of OVA-specific IgE antibodies was also significantly reduced in AcCystatin-treated rats compared with untreated asthmatic rats. The AcCystatin treatment was associated with a significant increase in IL-10 levels. Our present findings provide the first demonstration that AcCystatin is an effective agent in the prevention and treatment of the airway inflammation associated with asthma.

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Section: Discussionmentioning

confidence: 99%

AcCystatin, an immunoregulatory molecule from Angiostrongylus cantonensis, ameliorates the asthmatic response in an aluminium hydroxide/ovalbumin-induced rat model of asthma

Yang

et al. 2014

Parasitol Res

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“…Chronic asthma is accompanied by thickening of the bronchial walls, epithelial damage, subepithelial fibrosis, increased deposition of extracellular matrix proteins, and increased activity of various cytokines and growth factors, all of which contribute to airway remodeling. Airway inflammation is a hallmark characteristic of chronic asthma and is closely associated with Th2 cell activation and the release of cytokines, including IL-4, IL-5 and IL-13, which in turn accelerate the airway inflammatory cell infiltration (23). At elevated levels, Th2 cytokines can stimulate the production of growth factors, including VEGF and TGF-β1, thereby resulting in pulmonary fibrosis (24,25).…”

Section: Discussionmentioning

confidence: 99%

Bangpungtongseong-san, a traditional herbal medicine, attenuates chronic asthmatic effects induced by repeated ovalbumin challenge

Lee

Shin

Jeon

et al. 2014

International Journal of Molecular Medicine

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Airway remodeling is characterized by airway wall thickening, subepithelial ﬁbrosis, increased smooth muscle mass, angiogenesis and increased mucus secretion, which can lead to chronic and obstinate asthma and can obstruct pulmonary function. In this study, the effects of Bangpungtongseong-san water extract (BPTS) on airway remodeling were examined using a murine model of bronchial asthma induced by ovalbumin (OVA) challenge. We focused on the effects of BPTS on the regulation of chronic asthma. BALB/c mice were randomly assigned to 5 groups, some of which were sensitized and challenged with OVA for 4 weeks. After the final ovalbumin challenge, typical asthma-like morphological changes were observed in the lung tissue with hematoxylin and eosin staining, periodic acid-Schiff, as well as with Masson's trichrome staining. The levels of transforming growth factor-β1 (TGF-β1) and Smad3 were assessed by immunohistochemistry and western blot analysis. The expression levels of vascular endothelial growth factor (VEGF) and adhesion molecules, such as intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 were also detected by western blot analysis. Our results revealed that BPTS reduced the OVA-induced increase in the infiltration of leukocytes, mucus hyperplasia and collagen deposition. Compared with the OVA-challenged group, the BPTS group had lower expression levels of adhesion molecules, TGF-β1, Smad3 and VEGF proteins in the lung tissues. The results of the current study suggest that BPTS prevents asthma airway remodeling in chronic asthma by inhibiting the activation of the TGF-β1-Smad3-signaling pathway, as well as the expression of VEGF and adhesion molecules. BPTS may thus be a potential drug for the treatment of patients with changes that occur in the airways due to severe asthma.

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“…Allergen exposure activates T helper 2 (Th2) cells to release Th2 cytokines, such as interleukin (IL)-4, IL-5, and IL-13 (3). These mediators increase inflammatory cell infiltration of the airway, immunoglobulin (Ig)E switching, and chemokine release, which lead to mucus hypersecretion in the bronchiolar epithelial region (4). Thus, Th2 cytokine reduction is the objective of antiasthmatic agents, including herbal medicines (3,5,6).…”

Section: Introductionmentioning

confidence: 99%

EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma

Shin

Lee

Jeon

et al. 2013

International Journal of Molecular Medicine

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Abstract. EBM84 is a traditional herbal medicine and a combination of extracts obtained from Pinellia ternata and Zingiber officinale. It is traditionally used to treat vomiting, nausea, sputum and gastrointestinal disorders, and functions is an effective expectorant. In this study, we evaluated the protective effects of EBM84 on asthmatic responses, particularly mucus hypersecretion in an ovalbumin (OVA)-induced murine model of asthma. We also analyzed EBM84 composition using high performance liquid chromatography. Animals were sensitized on days 0 and 14 via intraperitoneal injection using 20 µg OVA. On days 21, 22 and 23 after initial sensitization, the mice received an airway challenge with OVA (1% w/v in PBS) for 1 h using an ultrasonic nebulizer (NE-U12). EBM84 was administered by gavage to the mice at doses of 16.9, 33.8 and 67.5 mg/kg once daily from days 18 to 23. EBM84 administration significantly lowered elevated levels of interleukin (IL)-4, IL-13, eotaxin and immunoglobulin (Ig)E in the bronchoalveolar lavage fluid or plasma. Airway inflammation and mucus hypersecretion were attenuated following EBM84 administration. EBM84 also inhibited the overexpression of mucin 5AC (MUC5AC) induced by OVA challenge in lung tissue. This result was consistent with the immunohistochemistry results. Our results indicate that EBM84 effectively inhibited airway inflammation and mucus hypersecretion via the downregulation of T helper 2 (Th2) cytokines, which reduced MUC5AC expression. Therefore, EBM84 has potential as a useful medicine for the treatment of allergic asthma.

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Effects of astilbic acid on airway hyperresponsiveness and inflammation in a mouse model of allergic asthma

Cited by 29 publications

References 31 publications

AcCystatin, an immunoregulatory molecule from Angiostrongylus cantonensis, ameliorates the asthmatic response in an aluminium hydroxide/ovalbumin-induced rat model of asthma

AcCystatin, an immunoregulatory molecule from Angiostrongylus cantonensis, ameliorates the asthmatic response in an aluminium hydroxide/ovalbumin-induced rat model of asthma

Bangpungtongseong-san, a traditional herbal medicine, attenuates chronic asthmatic effects induced by repeated ovalbumin challenge

EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma

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