Effects of Avian Infectious Bronchitis Virus (IBV) on Tracheal Organ Cultures

Wm, Colwell; Pd, Lukert

doi:10.2307/1588596

Cited by 28 publications

(13 citation statements)

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“…Similar changes were observed in expiants of tracheas in the present study. Colwell & Lukert (1969) and Darbyshire et al (1976) examined the cytopathology following IBV inoculation of trachéal OCs and described rounding and sloughing off of the ciliated epithelial cells, similar to that observed here, but the present study is believed to be the first detailed histological examination of the effect of different IBV strains on trachéal explants. All the nine isolations of IBV obtained in this study were from flocks of broilers or commercial layers.…”

Section: Discussionsupporting

confidence: 83%

Characterisation of strains of infectious bronchitis virus isolated in Chile

Cubillos

Ulloa²,

Cubillos³

et al. 1991

Avian Pathology

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SUMMARYNine isolates of infectious bronchitis (IB)-like viruses were made from 23 flocks (broilers or layers) in Chile experiencing the types of disease problems commonly associated with IBV. Their identity as IB viruses was confirmed. The histological changes they caused in tracheal organ cultures (OC) are described.Serum neutralisation tests performed in embryonated eggs (α-method) suggested that four of the isolates were serologically related to the Massachusetts (Mass) serotype of IBV and one to Connecticut. The four other strains were examined further by a serum neutralisation test in OC (ß-method). One was found to be of the Mass serotype but the others were found to be unrelated antigenically to a wide range of IBV serotypes isolated in many countries over a number of years. One of these three strains and the Mass strain, when given intranasally to 8-day-old specified pathogen free chickens together with pathogenic serotypes of E.coli, caused some mortality and considerable morbidity. The H120 vaccine strain was found not to protect completely against challenge with these four strains 21 days later.

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Section: Discussionsupporting

confidence: 83%

Characterisation of strains of infectious bronchitis virus isolated in Chile

Cubillos

Ulloa²,

Cubillos³

et al. 1991

Avian Pathology

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“…Many papers have been published on the duration of IBV infections in the chicken (Hofstad & Yoder, 1966;Doherty, 1967;Colwell & Lukert, 1969;Cook, 1971;Crinion et al, 1971;Jones & Jordan, 1972;Hawkes et al, 1983;Smith et al, 1985;ElHouadfi et al, 1986;Jones & Ambali, 1987;Bumstead et al, 1989;Ambali & Jones, 1990;Otsuki et al, 1990;Nakamura et al, 1991;Owen et al, 1991;Bhattacharjee et al, 1995;Dhinakar Raj & Jones, 1997a;De Wit et al, 1998b). These studies involve a large variety of sampled organs, different virus strains, different doses, etc., and can therefore be expected to present partly conflicting data about the intervals in which the virus can be detected in the various organs.…”

Section: Time Between Start Of Infection and Samplingmentioning

confidence: 99%

“…Tracheal organ cultures, usually prepared from 20-dayold (SPF) embryos, have proved very successful for the isolation, titration and serotyping of IBV (Cook, 1984) because no adaptation of field strains is required for growth and induction of (non-specific) ciliostasis. Ciliostasis, easily observed by lowpower microscopy, usually occurs within 3 to 4 days after inoculation, but this period can differ between strains, inoculation dose (Colwell & Lukert, 1969) and, possibly, genetic factors of the host (Otsuki et al, 1990). The presence of IBV in field samples has to be confirmed by an IBVspecific test, as ciliostasis can also be induced by many other agents.…”

Section: Virus Isolation (Multiplication and Detection Of Infectious mentioning

confidence: 99%

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

167

128

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The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protectotyping, serotyping, epitope-typing and genotyping.

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“…Chicken tracheal organ cultures have been shown to be susceptible to infection with AIB virus and may be employed as an accurate assay system if eiliostasis is used as the criterion of infection (12,4,13,2,7). Furthermore, JOHNSO~ ~ and N~wMA~ (13) found that tracheal explants provided a better assay system than embryonated chicken eggs because the results were more reproducible.…”

Section: Introductionmentioning

confidence: 99%

The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus

Cook

Darbyshire

Peters

1976

Archives of Virology

185

150

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A study has been made of the use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis (AIB) virus from both naturally and experimentally infected chickens. Six strains of AIB virus were investigated, 3 of which had been isolated from natural outbreaks of disease. Two of the virus isolations from the outbreaks of AIB were made directly into tracheal organ cultures without passage in embryonated eggs. Organ cultures prepared from 20-day-old embryos were used since they were found to be somewhat more sensitive in virus assay than those derived from chickens of up to 31 days of age. Ciliostasis, which was used as the marker of infectivity, was complete by 3 days after inoculation with each strain of virus examined. Virus could be isolated from both respiratory and non-respiratory tissue in tracheal organ cultures and these cultures were found to be at least as sensitive as 9-day-old embryonated eggs in detecting AIB virus either in pathological material or in serial dilutions. When virus was assayed in both systems, the titres were very similar. It is considered, therefore, that chicken embryo tracheal organ cultures offer a reliable alternative system to embryonated eggs for studying AIB virus.

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Effects of Avian Infectious Bronchitis Virus (IBV) on Tracheal Organ Cultures

Cited by 28 publications

References 0 publications

Characterisation of strains of infectious bronchitis virus isolated in Chile

Characterisation of strains of infectious bronchitis virus isolated in Chile

Detection of infectious bronchitis virus

The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus

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