Effects of Binahong (Anredera cordifolia (Tenore) Steenis) Extracts on the Levels of Malondialdehyde (MDA) in Cataract Goat Lenses

Abstract

Cataracts are one of the most causes of blindness in the world. Oxidative stress can form pathological conditions such as cataracts. This oxidative stress ability can be measured by the malondialdehyde (MDA) biomarker. Binahong leaves (Anredera cordifolia (Tenore) Steenis) are native plants from Indonesia that are used to treat various diseases including cataract treatment. Binahong leaf (Anredera cordifolia (Tenore) Steenis) has a high amount of flavonoids and is rich in antioxidants that can be used to treat cataracts. Objective. The purpose of this study was to assess the effect of binahong leaf extract on the levels of MDA in a goat lens with cataract-induced material. Method. As many as possible, 40 goat eye lenses were divided into several groups, namely, group I normal lenses as controls (glucose 5.5 mM), group II lenses were cataract induced with glucose concentration of 55 mM, group III lenses with glucose 55 mM + binahong leaf extract (100 μg/ml), group IV lens with glucose 55 mM + binahong leaf extract (200 μg/ml), and group V lens with glucose 55 mM + quercetin (positive control). Biochemical parameters measured in the lens homogenate are malondialdehyde lens morphology in all groups’ observations and comparisons made. Results. The results of the study found that the lens group with the addition of binahong extract showed more results transparency compared to lens groups induced by glucose concentrations of 55 mM). This shows that the diabetic cataract group experienced high oxidative stress due to the accumulation of sorbitol compounds derived from glucose which caused turbidity in the goat eye lens and increased levels of lens MDA. Binahong levels at concentrations of 100 or 200 can inhibit MDA production. Conclusion. Binahong (Anredera cordifolia (Tenore) Steenis) extract has the ability to inhibit the production of MDA levels. In glucose-induced goat lenses, binahong extract and quercetin show antioxidant and anticataract properties.

Kolaviron neuroprotective effect against okadaic acid-provoked cognitive impairment

“…Quantity of MDA as a factor of lipid peroxidation was measured in the presence of 0.5 % thiobarbiturate and 20 % trichloroacetic acid in a thermoblock (95ᵒC) for 30 min. After cooling and centrifuging (1500× g ) at 25ᵒC for 5 min, absorption was obtained at 535 nm and with tetraethoxypropane as the standard [ 37 ].…”

Natural Antioxidant Activities of Plants in Preventing Cataractogenesis

“…According to the ethanol extract of the leaves of the binahong plant, the lens group of the goat lens induced with glucose and the addition of the binahong (Anredera cordifolia (Tenore) Steenis) extract exhibited more transparent results than the lens group induced with 55 mM glucose concentration. Binahong can suppress malondialdehyde generation at doses of 100 or 200 μg/mL [ 101 ]. In another study, it was stated that Lupeol, a pentacyclic triterpenoid isolated from Vernonia cinereal , was effective in the treatment of cataracts in the eye lens of Sprague Dawley rats induced by selenite from the results of testing biochemical parameters such as activity of SOD, CAT, GPx, GR, GST, Ca 2+ ATPase, glutathione, ROS, and lipid peroxidation product (malondialdehyde) were found to be effective in the treatment of cataracts with lupeol [ 100 , 102 ].…”

Antioxidant and Hepatoprotective Activity of Aerides odorata Lour. on Alcohol Induced Liver Damage in Rats

“…In this process, they are causing oxidative stress by reacting with different cellular components such as lipids, proteins, nucleotides and other macromolecules [28,37,38] . During oxidative stress, the levels of the oxidative enzyme will increase, and antioxidant enzymes levels will decrease [39,40] , and the cellular component damage will occur through lipid peroxidation, i.e., increased levels of TBARS levels indicated by MDA [41,42] . The antioxidant activity of A. odorata extracts results showed that good inhibition of free radicals in in vitro study against tested free radicals and in in vivo study results supports the in vitro results with their capability in ameliorating the levels of the antioxidant enzyme, i.e., rise in catalase and SOD levels and decrease the cellular component damages in the body (Tables 3 and 4).…”