Effects of both vitamin C and mechanical stimulation on improving the mechanical characteristics of regenerated cartilage

Omata, Seiji; Sonokawa, Shogo; Sawae, Yoshinori; Murakami, Teruo

doi:10.1016/j.bbrc.2012.07.019

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…Cartilage specific proteins including collagen type II and aggrecan can also be elevated after treatment with these agents (Bhatti et al 2013). Furthermore, favorable results were obtained after application in cartilage repair (Omata et al 2012). These findings suggest that antioxidants are promising candidates to replace growth factors in ACI.…”

Section: Introductionmentioning

confidence: 70%

Chondroprotective Effects of Taurine in Primary Cultures of Human Articular Chondrocytes

Liu

et al. 2015

Tohoku J. Exp. Med.

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Articular cartilage is characterized by the lack of blood vessels and has a poor self-healing potential. Limited cell numbers and dedifferentiation of chondrocytes when expanded in vitro are the major obstacles of autologous chondrocyte implantation. Autologous chondrocyte implantation is a cell-based treatment that can be used as a second-line measure to regenerate chondral or osteochondral defects in younger, active patients. There is an urgent need to find an effective chondrogenic protection agent alleviating or inhibiting chondrocyte dedifferentiation. In this study, we explored the effect of taurine (2-aminoethane sulfonic acid) on proliferation and phenotype maintenance of human articular chondrocytes by analyzing the cell proliferation, morphology, viability, and expression of cartilage specific mRNAs and proteins. Primary chondrocytes were isolated from human articular cartilage tissues. Results showed that taurine effectively promoted chondrocyte growth and enhanced accumulation of glycosaminoglycans and collagens in the conditioned media of chondrocytes. Moreover, taurine exposure caused significant increases in the relative expression levels of mRNAs for cartilage specific markers, including aggrecan, collagen type II and SOX9. Aggrecan is a cartilage-specific proteoglycan, and SOX9 is a chondrogenic transcription factor. In contrast, the mRNA expression of collagen type I, a marker for chondrocyte dedifferentiation, was significantly decreased in cells treated with taurine, indicating that taurine inhibits the chondrocyte dedifferentiation. This study reveals that taurine is effective in proliferation promotion and phenotype maintenance of chondrocytes. Thus, taurine may be a useful pro-chondrogenic agent for autologous chondrocyte implantation in the treatment of cartilage repair.

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Section: Introductionmentioning

confidence: 70%

Chondroprotective Effects of Taurine in Primary Cultures of Human Articular Chondrocytes

Liu

et al. 2015

Tohoku J. Exp. Med.

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“…Antioxidants were identified to exert cytoprotective effects in vitro and in vivo (29,30). AA, a widely used antioxidant, was revealed to delay the progression of age-associated diseases, which are highly susceptible to oxidative stress (19,21). However, it is unknown whether AA can provide any resistance to oxidative stress for the protection of chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The reactions were conducted with the following thermocycling conditions: at 95˚C for 10 min, followed by 45 cycles of 95˚C for 10 s, 56˚C for 30 s and extension for 10 s at 72˚C. The data were assessed using the 2 -∆∆Ct method for relative quantification (21,22). The GAPDH gene was used as the endogenous control for normalization.…”

Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

“…Previous studies have revealed that AA attenuates oxidative stress in diabetic aged rats (19,20). AA also exerts a role in chondroprotection: The application of AA and mechanical stimulation in combination was revealed to improve the mechanical characteristics of regenerated cartilage (21). AA was revealed to markedly affect the expression of proteins with specific functions in human articular chondrocytes under conditions of homogentisic acid-induced stress, which may be mediated by protein oxidation (22).…”

Section: Introductionmentioning

confidence: 99%

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Ascorbic acid provides protection for human chondrocytes against oxidative stress

Chang

Huo

et al. 2015

Molecular Medicine Reports

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Abstract. Oxidative stress is considered to be an important cause of dysfunction in chondrocytes and articular cartilage degradation, which leads to the pathogenesis of osteoarthritis (OA) and cartilage aging. The present study aimed to assess the effects of the widely applied antioxidant, ascorbic acid (AA), on human chondrocytes against hydrogen peroxide (H 2 O 2 ) in vitro. Using annexin V-fluorescein isothiocyanate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and senescence-associated β-galactosidase assays, the present study identified that AA reduced apoptosis, reduced the loss of viability and markedly decreased H 2 O 2 -mediated senescence in cells treated with H 2 O 2 . Furthermore, AA not only stimulated the expression levels of collagens and proteoglycans, but also inhibited the differentiation of chondrocytes under conditions of oxidative stress. In addition, reverse transcription-quantitative polymerase chain reaction and western blotting demonstrated that AA decreased the activity of nrf2, NF-κB, AP1 and matrix metalloproteinase-3, which is stimulated by H 2 O 2 . In conclusion, AA efficiently protected human chondrocytes against damage induced by H 2 O 2 by regulating multiple regulatory pathways.

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“…The PCB underwent systematic validation revealing safe and reliable functionality to ensure defined dynamic mechanical loading of viable engineered tissues. As compared to previously described systems (Rath et al, 2008 ; Ballyns and Bonassar, 2010 ; Sittichokechaiwut et al, 2010 ; Sun et al, 2010 ; Lujan et al, 2011 ; Matziolis et al, 2011 ; Omata et al, 2012 ; Petri et al, 2012 ; Puetzer et al, 2012 ; Shahin and Doran, 2012 ), the PCB exhibits a broader operational range. It allows for physiological mechanical compression in a consistent and reliable manner in a range from approximately 10 N up to 1 kN, while maintaining compact dimensions.…”

Section: Discussionmentioning

confidence: 69%

Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing

Hoffmann

Feliciano

Martín

et al. 2015

Front. Bioeng. Biotechnol.

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Secondary bone fracture healing is a physiological process that leads to functional tissue regeneration via endochondral bone formation. In vivo studies have demonstrated that early mobilization and the application of mechanical loads enhances the process of fracture healing. However, the influence of specific mechanical stimuli and particular effects during specific phases of fracture healing remain to be elucidated. In this work, we have developed and provided proof-of-concept of an in vitro human organotypic model of physiological loading of a cartilage callus, based on a novel perfused compression bioreactor (PCB) system. We then used the fracture callus model to investigate the regulatory role of dynamic mechanical loading. Our findings provide a proof-of-principle that dynamic mechanical loading applied by the PCB can enhance the maturation process of mesenchymal stromal cells toward late hypertrophic chondrocytes and the mineralization of the deposited extracellular matrix. The PCB provides a promising tool to study fracture healing and for the in vitro assessment of alternative fracture treatments based on engineered tissue grafts or pharmaceutical compounds, allowing for the reduction of animal experiments.

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Effects of both vitamin C and mechanical stimulation on improving the mechanical characteristics of regenerated cartilage

Cited by 16 publications

References 23 publications

Chondroprotective Effects of Taurine in Primary Cultures of Human Articular Chondrocytes

Chondroprotective Effects of Taurine in Primary Cultures of Human Articular Chondrocytes

Ascorbic acid provides protection for human chondrocytes against oxidative stress

Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing

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