Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure

Xu, Xiaofeng; Tan, Yong; Jiang, Guilin; Chen, Xuanyi; Lai, Rensheng; Zhang, Lurong; Liang, Guoqiang

doi:10.1186/s13020-017-0131-3

Cited by 20 publications

(14 citation statements)

References 54 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rats (7 rats/group) were intragastrically treated with control solution or one of three doses of quercetin (12.5 mg/kg, 25 mg/kg, and 50 mg/kg) for 90 days. After quercetin administration, estrous cycles of all rats (15 months old) stopped at either estrus (46.43%) or diestrus (53.57%) (Table 3 ), indicating that the estrous cycle had been terminated in all rats [ 17 ]. Furthermore, no obvious differences in the estrous cycle were observed between the control group and the groups treated with the three doses of quercetin, indicating that quercetin did not affect the estrous cycle of menopausal rats.…”

Section: Resultsmentioning

confidence: 99%

Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro

et al. 2018

View full text Add to dashboard Cite

BackgroundMenopause is the most important sign of aging in women, and the ovary is the organ most sensitive to aging. Quercetin is a potential antioxidant and free radical scavenger that is widely found in fruits, vegetables, and leaves. However, the effect of quercetin on ovarian aging has not been elucidated, and the mechanism underlying its antioxidative effect remains unclear. The purpose of the current study was to investigate whether quercetin protects ovarian function by decreasing oxidative stress.MethodsIn an in vivo experiment, female menopausal rats (12 months old) were intragastrically administered quercetin at three doses (12.5 mg/kg, 25 mg/kg, and 50 mg/kg) for 90 days, and the estrous cycles were determined by vaginal smearing. In an in vitro experiment, rat primary ovarian granulosa cells were cultured and treated with H2O2 (400 μM) alone or H2O2 plus quercetin at 5 μM, 20 μM, or 50 μM. The levels of the hormones estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were detected by radioimmunoassay. The serum levels of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-PX) and glutathione S-transferase (GST) were examined. The expression levels of the oxidative stress-related genes SOD-1, catalase (CAT) and glutathione synthetase (GSS) in the ovaries and ovarian granulosa cells were detected by Western blot.ResultsThe in vivo results demonstrated that quercetin had no effects on ovarian morphology, hormone secretion, or the estrous cycle in menopausal rats. Although no significant changes were detected in the serum levels of T-AOC, SOD, GSH, GSH-PX, and GST between the quercetin and control groups, the mRNA and protein expression levels of the oxidative stress-related genes SOD-1, CAT and GSS in menopausal rat ovaries were increased by low-dose quercetin. Moreover, the in vitro results demonstrated that quercetin significantly rescued the decrease in cell viability by H2О2-induced oxidative stress and enhanced the H2O2-induced decrease in expression of oxidative stress-related proteins.ConclusionsTogether, the results of this study indicated that quercetin increased the antioxidant capacity of the ovary by upregulating the expression of some oxidative stress-related genes both in vivo and in vitro.

show abstract

Section: Resultsmentioning

confidence: 99%

Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Rats that went through at least two consecutive regular estrous cycles were included in the experiments. The length of an estrous cycle was determined as the number of days between two non-consecutive days during which estrus cytology was observed [25]. The average length of regular estrous cycles in the rats was 4.4 days ( n = 18), ranging from 4 to 5 days.…”

Section: Methodsmentioning

confidence: 99%

“…Several studies have suggested that the efficacy of MSC transplantation on POI is mainly attributed to the paracrine mechanism [9, 10]. MSCs can secrete a variety of paracrine/autocrine factors, including growth factors, chemokines, and colony-stimulating factors, which are called secretomes, that mediate diverse functions [23–25]. Some studies have shown that the MSC secretome could be therapeutic for the treatment of diseases [26–28].…”

Section: Introductionmentioning

confidence: 99%

“…Some studies have shown that the MSC secretome could be therapeutic for the treatment of diseases [26–28]. Various paracrine factors secreted by MSCs can act directly, triggering intracellular mechanisms of injured cells, or act indirectly, promoting the secretion of functional active mediators in neighboring cells, which may attenuate tissue damage, inhibit apoptosis and fibrosis, promote angiogenesis, and modulate immune responses [25, 29]. MSC-conditioned media (CM) contains various factors and microvesicles secreted by MSCs that could be applicable in regenerative medicine [29].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation improves ovarian function in rats with premature ovarian insufficiency (POI) at least partly through a paracrine mechanism

et al. 2019

View full text Add to dashboard Cite

BackgroundChemotherapy can induce premature ovarian insufficiency (POI) and reduce fertility in young female patients. Currently, there is no effective therapy for POI. Human amnion-derived mesenchymal stem cells (hAD-MSCs) may be a promising seed cell for regenerative medicine. This study investigated the effects and mechanisms of hAD-MSC transplantation on chemotherapy-induced POI in rats.MethodsChemotherapy-induced POI rat models were established by intraperitoneal injection of cyclophosphamide. Seventy-two female SD rats were randomly divided into control, POI, and hAD-MSC-treated groups. hAD-MSCs were labeled with PKH26 and injected into the tail veins of POI rats. To examine the underlying mechanisms, the differentiation of transplanted hAD-MSCs in the POI ovaries was analyzed by immunofluorescent staining. The in vitro expression of growth factors secreted by hAD-MSCs in hAD-MSC-conditioned media (hAD-MSC-CM) was analyzed by ELISA. Sixty female SD rats were divided into control, POI, and hAD-MSC-CM-treated groups, and hAD-MSC-CM was injected into the bilateral ovaries of POI rats. After hAD-MSC transplantation or hAD-MSC-CM injection, serum sex hormone levels, estrous cycles, ovarian pathological changes, follicle counts, granulosa cell (GC) apoptosis, and Bcl-2, Bax, and VEGF expression in ovaries were examined.ResultsPKH26-labeled hAD-MSCs mainly homed to ovaries after transplantation.hAD-MSC transplantation reduced ovarian injury and improved ovarian function in rats with POI. Transplanted hAD-MSCs were only located in the interstitium of ovaries, rather than in follicles, and did not express the typical markers of oocytes and GCs, which are ZP3 and FSHR, respectively. hAD-MSCs secreted FGF2, IGF-1, HGF, and VEGF, and those growth factors were detected in the hAD-MSC-CM. hAD-MSC-CM injection improved the local microenvironment of POI ovaries, leading to a decrease in Bax expression and an increase in Bcl-2 and endogenous VEGF expression in ovarian cells, which inhibited chemotherapy-induced GC apoptosis, promoted angiogenesis and regulated follicular development, thus partly reducing ovarian injury and improving ovarian function in rats with POI.ConclusionshAD-MSC transplantation can improve ovarian function in rats with chemotherapy-induced POI at least partly through a paracrine mechanism. The presence of a paracrine mechanism accounting for hAD-MSC-mediated recovery of ovarian function might be attributed to the growth factors secreted by hAD-MSCs.

show abstract

“…Speaking of which, management of POF in western medicine nowadays involves immunosuppressive treatment, hormone therapy such as hormone replacement therapy (HRT), and ovarian transplantation [ 13 , 14 ]. Immunosuppressive treatment presents possibility of resumption of ovarian function; nevertheless, treatment with immunosuppressive agents failed to reverse the course of the ovarian autoimmunity or enhance the ovarian response to gonadotropins [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment

Yuemaier

Tuerhong

Keremu

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

This study aimed to establish and explore the biological basis of abnormal phlegmatic syndrome with premature ovarian failure (POF) model in rats based on the Uighur medicine (UM) in the first place and investigate the effects of unique herbal medicine, Balgham Munziq (BMq). Mature female Wistar rats were fed with spinach and coriander in cold and humid condition for approximately 20 weeks until abnormal phlegmatic syndrome (APS) model was established. When APS model was confirmed by Uighur medical experts, APS with POF disease rats were subdivided into APS with POF disease model group and APS with POF disease treated with BMq group; the rest of them were subdivided into APS model group and APS treated with BMq group. The results show that biological characteristics of animals in the course of modeling period were in accordance with clinical features of abnormal phlegmatic syndrome (APS) in Uighur medicine. Herbal medicine BMq not only reverted reproductive hormone levels disorders but also improved the function of hypothalamic-pituitary-ovarian axis and regulated secretion of monoamine neurotransmitters. APS is most likely to cause pathological changes of hypothalamic-pituitary-ovarian axis and lead to the occurrence of POF and BMq is effective in the treatment of APS with POF disease.

show abstract

Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure

Cited by 20 publications

References 54 publications

Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro

Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro

Human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation improves ovarian function in rats with premature ovarian insufficiency (POI) at least partly through a paracrine mechanism

Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment

Contact Info

Product

Resources

About