Effects of Cadmium and Phenol on Motility and Ultrastructure of Sea Urchin and Mussel Spermatozoa

Au, Doris W.T.; Chiang, Michael; Wu, Rongben

doi:10.1007/s002449910060

Cited by 72 publications

(34 citation statements)

References 29 publications

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“…Moreover, LM fish's spermatozoa revealed the presence of few numbers and large sized mitochondria in front of the proximal centriole toward the lateral aspects of the nucleus. The enlarged size of the mitochondria appeared to be a considerable hypertrophy and a sign of acclimatization of fish in these samples rather than RN [48,49]. The bilateral fins of the spermatozoa that formed by the plasma membrane extended from the flagellum is very important for perfect motility and exhibits higher fertilization capacity [40].…”

Section: Discussionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

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IntroductionThe importance of Nile tilapia, Oreochromis niloticus in aqua cultures is due to its spreading in most countries of the world [1]. It breads in captivity and wide variety of water conditions [2]. Lake Manzalah (LM) is one of the most important lakes in Egypt that provides more than 70% of the total fishery of the country [3] and considered as a highly polluted with toxic heavy metals and pesticides due to progressive increase of industrial and agricultural drainage, as well as sewage out fall from the surrounding governorates [4]. The mean values of some heavy metals (Cd, Pb, Fe, Cr and Mn) recorded in LM were higher than the world permissible levels [5] and according to the Egyptian laws [6]. Such heavy metals are not lethal to fish, but concentrated in their flesh and create a hazard to the consumer [7,8]. Cd, Pb, Zn, and Cr are considered the main causes of pollution in aquatic ecosystem [9]. The serious effect of these metals is derived from their persistence, toxicity and bioaccumulation, and consequently exert dangerous problem to man [10]. Experimental studies on heavy metals proved that they could impair the respiratory functions of the gills; consequently death in fish is probably caused by tissue hypoxia [11]. Metals affect not only the fish morphology, but also all biological activities [12]. Among the heavy metals; Cd and Pb are the serious heavy metals that produce histopathological alterations including liver damage [13], respiratory dysfunctions [14], testicular and ovarian alterations [15][16][17]. These pathological alterations were primarily attributed to damage of cell membranes which allows higher uptake of Cd and Pb and thus the injury extends to more critical targets [18]. Many studies focused on bioaccumulation of heavy metals in fish and biological studies. Thus we aimed in this work to study the testicular histopathological alterations of Oreochromis niloticus using light microscope and the mature sperm using TEM. Materials and Methods Samples collectionSamples of water and 48 specimen of Nile tilapia, Oreochromis niloticus about 14-20 cm in length and 120-200 g in weight were collected from two areas; Demitta branch of RN at Mansoura city as a control site and LM as a polluted area during the spawning season from March-June and transported to the laboratory in tanks provided with oxygen pump. The fish were weighed, dissected and the testes were Abstract Background: Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing serious threat to the survival of aquatic organisms including fish. Lake Manzala (LM) is one of the Egypt's northern Delta Lakes situated on the Mediterranean Coast of the Delta. It is affected by drainage water polluted by different heavy metals that their concentrations exceed the maximum world permissible levels.

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Section: Discussionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

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“…The characteristics of sperm, collected after mollusk sperm shedding, were estimated in a few species: mussel (Mytilus edulis, Everett et al 2004;Jha et al 2007), Pacific oyster (Cosson et al 2008a;Suquet et al 2012) and sea urchin (Anthocidaris crassispina; Au et al 2000). Sperm fertilisation capacity of two Caribbean scallops (Argopecten nucleus and Nodipecten nodosus) was not influenced by stimulation technique, including temperature shock and serotonin or prostaglandin injection (Velasco et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Suquet

Quéré

Mingant

et al. 2013

Aquat. Living Resour.

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-Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (VAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, VAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and VAP was assessed, suggesting a sperm 'maturation process' along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 min incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher VAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, VAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, VAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 ± 7%, 162 ± 15 µm s −1 , 0.33 ± 0.12 and 212 ± 133 nmol ×10 −9 spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm "maturation process" along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase.

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“…Contudo, os resultados variaram de 81,92 a 97,14% de fertilização artificial, taxas estas consideradas elevadas e adequadas para o padrão da espécie em condições normais de criação (Baldisserotto e Radunz-Neto, 2004).…”

Section: Resultados E Discussão Resultados E Discussão Resultados E Dunclassified

“…A mensuração da concentração espermática do sêmen foi realizada utilizando-se o método da contagem de células espermáticas (Streit Jr. et al, 2004), em câmara hematimétrica de Neubauer (CSPZ) (espermatozoides (SPZ) mL -1 ) (Billard et al, 1996). Para a mensuração da concentração espermática, utilizou-se amostra de 5 µL do sêmen que foi diluída em 5000 µL de formol salina tamponado, conforme Bombardelli et al (2006), resultando em uma diluição de 1:1000.…”

Section: Methodsunclassified

Efeito do chumbo sobre a fertilização artificial e incubação de ovos de jundiá cinza (<em>Rhamdia quelen</em>)

Hilbig

Bombardelli

Sanches

et al. 2008

Acta Sci. Anim. Sci.

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Effects of Cadmium and Phenol on Motility and Ultrastructure of Sea Urchin and Mussel Spermatozoa

Cited by 72 publications

References 29 publications

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Efeito do chumbo sobre a fertilização artificial e incubação de ovos de jundiá cinza (<em>Rhamdia quelen</em>)

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