Effects of Calcium Ions on the Malate-Aspartate Shuttle in Slow-Cooled Boar Spermatozoa

Nishimura, Kazuhiko

doi:10.1095/biolreprod49.3.537

Cited by 5 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our study shows positive correlations between La, Ce, Eu, Gd, and Ca concentrations in semen. Lanthanides act as Ca analogues in biological systems, and trivalent lanthanide ions can replace Ca at metal-binding sites (Wang et al 1982 ; Lansman 1990 ; Nishimura 1993 ; Reeves and Condrescu 2003 ; Ardón et al 2009 ; Carrillo-López et al 2010 ). Seminal La, Ce, and Gd concentrations were positively associated with progressive motility and percentage of normal spermatozoa and negatively with sperm concentration.…”

Section: Discussionmentioning

confidence: 99%

Determination of Rare Earth Elements in Human Sperm and Association with Semen Quality

Marzec-Wróblewska

Kamiński

Łakota

et al. 2015

Arch Environ Contam Toxicol

View full text Add to dashboard Cite

The aim of the present study was to measure lanthanum (La), cerium (Ce), europium (Eu), and gadolinium (Gd) concentrations in human semen and correlate the results with sperm quality. The median semen content of La was 19.5 µg kg−1 dry weight (dw) (range 2.27–269), of Ce was 41.9 µg kg−1 dw (range 4.52 to 167), of Eu was 0.68 µg kg−1 dw (range 0.06–1.95), of Gd was 3.19 µg kg−1 dw (range 0.38–12.0), and of calcium (Ca) was 4063 mg kg−1 dw (range 484–17,191). Concentrations of La, Ce, Eu, Gd, and Ca were significantly lower in nondrinkers’ semen than in semen from drinkers. Significant differences were detected between La, Ce, Eu, Gd, and Ca concentrations in semen from nondrinkers and moderate drinkers. Concentrations of La, Ce, and Gd in semen of short-term smokers were significantly lower than those in extremely long-term smokers. Significant differences were also detected between La concentration in semen from a group of short-term smokers and that of a group of long-term smokers. Positive correlations were found between La, Ce, Eu, Gd, and Ca concentrations in semen. La, Ce, Gd, and Ca concentrations in semen were positively associated with progressive motility and percentage of normal spermatozoa. Positive correlations were found between Ca and sperm concentration. Concentrations of La, Ce, and Gd were negatively associated with sperm concentration, whilst Ca concentration was negatively associated with volume of ejaculate. At the examined level, La, Ce, Eu, and Gd did not affect sperm quality, whereas alcohol consumption and smoking might have increased the level of rare earth elements in semen.

show abstract

Section: Discussionmentioning

confidence: 99%

Determination of Rare Earth Elements in Human Sperm and Association with Semen Quality

Marzec-Wróblewska

Kamiński

Łakota

et al. 2015

Arch Environ Contam Toxicol

View full text Add to dashboard Cite

show abstract

“…Provavelmente, as amostras de sêmen cujo diluente foi acrescido de 0,4 e 4,0mM de CaCO 3 , Ca(OH) 2 , podem ter sofrido uma queda acentuada da motilidade após um dia de acondicionamento do sêmen, sem ter havido, por outro lado, uma alteração significativa na porcentagem de células vivas, uma vez que sobre essa característica, as possibilidades de influência positiva ou negativa, são multifatoriais. No reprodutor suíno, as modificações da permabilidade de membrana aos íons e, em particular, ao íon cálcio, seriam responsáveis pela mudança da motilidade dos espermatozoides (hiperativação) (VICENTE-CARRILLO et al, 2017), ou seja, modificações bioquímicas importantes para a função normal dos espermatozoides (NISHIMURA, 1993).…”

Section: Viabilidade Espermáticaunclassified

Efeito da Adição de Cálcio sobre a Hiperativação Espermática: Avaliação Computadorizada

et al. 2021

View full text Add to dashboard Cite

Estudos mostram que o íon cálcio é um mensageiro intracelular que regula fenômenos celulares fisiológicos, como a motilidade e fosforilação de enzimas. O objetivo do presente trabalho foi avaliar se a adição de diferentes tipos e concentrações de íons cálcio ao diluente BTS para sêmen suíno, promove hiperativação espermática. O sêmen de 4 varrões foi coletado durante dez semanas e seus parâmetros foram analisados. O experimento contou com a utilização de diferentes tipos de cálcio: carbonato de cálcio (CaCO3), hidróxido de cálcio (Ca(OH)2), fostato dibásico anidro de cálcio (CaHPO4), sulfato hidratado de cálcio (CaSO4.2H2O) e sulfato anidro de cálcio (CaSO4), distribuídos em diferentes concentrações: 0mM = controle; 0,4mM e 4mM. As amostras foram armazenadas a 17 oC e posteriormente incubadas a 37 oC para a avaliação computadorizada, realizada do D0 (dia da coleta) até D2 (último dia de incubação). Os testes de viabilidade e morfologia espermática foram realizados no D0 e D2. Os resultados para a concentração de 4 mM (CaHPO4, CaSO4.2H2O e CaSO4) indicaram um aumento da velocidade média da trajetória (≥40μm/s) e da freqüência de batimento cruzado (≥8,0Hz), além da redução da linearidade (≤35%). Concluiu-se que a adição de 4 mM de CaHPO4, CaSO4.2H2O e CaSO4 gerou a hiperativação das células espermáticas e não exerceu efeito negativo sobre a membrana espermática, ressaltando-se que a hiperativação das células espermáticas poderia aumentar a capacidade fertilizante dos espermatozoides em fertilização in vitro ou em processos de transferência de gametas intrafalopianos. Dessa forma, estudos adicionais são necessários para verificar a sua eficiência. Palavras chave: Espermatozoide. Motilidade. Suíno. Linearidade. Abstract Studies show that the calcium ion is intracellular messenger that regulates physiological cellular phenomena, such as motility and enzyme phosphorylation. The aim of the present study was to evaluate whether the addition of different types and concentrations of calcium ions to the BTS diluent for swine semen can promote sperm hyperactivation. The semen from 4 boars was collected during ten weeks and its parameters were analyzed. The experiment used different types of calcium: calcium carbonate (CaCO3), calcium hydroxide (Ca(OH)2), anhydrous calcium dibasic phosphate (CaHPO4), hydrated calcium sulfate (CaSO4.2H2O) and anhydrous calcium sulfate (CaSO4), distributed in different concentrations: 0mM = control; 0.4mM and 4mM. The samples were stored at 17 oC and then incubated at 37 oC for computerized evaluation, performed from D0 (collection day) to D2 (last day of incubation). The viability and sperm morphology tests were performed on D0 and D2. The results for the concentration of 4mM (CaHPO4, CaSO4.2H2O and CaSO4) indicated an increase in the average path velocity (≥40μm/s) and in the cross-beat frequency (≥8.0Hz), in addition to a reduction in linearity (≤35%). It was concluded that the addition of 4mM of CaHPO4, CaSO4.2H2O and CaSO4 generated the sperm cells hyperactivation and had no negative effect on the sperm membrane, emphasizing that the sperm cells hyperactivation could increase the sperm fertilizing capacity in in vitro fertilization or in intrafalopian gamete transfer processes. Thus, additional studies are necessary to verify its efficiency Keywords: Sperm. Motility. Swine. Linearity.

show abstract

The rate of L-lactate production: a feasible parameter for the fresh diluted boar semen quality analysis

Rigau

Piedrafita

Reverter

et al. 1996

Animal Reproduction Science

View full text Add to dashboard Cite

Effects of Calcium Ions on the Malate-Aspartate Shuttle in Slow-Cooled Boar Spermatozoa

Cited by 5 publications

References 24 publications

Determination of Rare Earth Elements in Human Sperm and Association with Semen Quality

Determination of Rare Earth Elements in Human Sperm and Association with Semen Quality

Efeito da Adição de Cálcio sobre a Hiperativação Espermática: Avaliação Computadorizada

The rate of L-lactate production: a feasible parameter for the fresh diluted boar semen quality analysis

Contact Info

Product

Resources

About