Effects of Calcium Silicate Endodontic Cements on Biocompatibility and Mineralization-inducing Potentials in Human Dental Pulp Cells

Chang, Seok Woo; Lee, So‐Youn; Ann, Hyo-Jung; Kum, Kee‐Yeon; Kim, Eun-Cheol

doi:10.1016/j.joen.2014.01.001

Cited by 81 publications

(83 citation statements)

References 44 publications

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“…The reparative tissues induced by MTA and Biodentine were homogenous, whereas those induced by Ca(OH) 2 were porous, suggesting a reparative process different from that induced by calcium silicate (14,31). Studies indicate greater tissue repair efficiency of calcium silicate compared with that of Ca(OH) 2 , probably because of the recruitment of pulp stem cells by the former.…”

Section: Clinical Researchmentioning

confidence: 94%

Tomographic Evaluation of Reparative Dentin Formation after Direct Pulp Capping with Ca(OH)2, MTA, Biodentine, and Dentin Bonding System in Human Teeth

Nowicka

Wilk

Lipski

et al. 2015

Journal of Endodontics

114

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Section: Clinical Researchmentioning

confidence: 94%

Tomographic Evaluation of Reparative Dentin Formation after Direct Pulp Capping with Ca(OH)2, MTA, Biodentine, and Dentin Bonding System in Human Teeth

Nowicka

Wilk

Lipski

et al. 2015

Journal of Endodontics

114

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“…These results are in agreement with those found in previous cytotoxicity studies. [26,27] BioAggregate showed no cytotoxic effect in the first day extracts. Seventh day extracts of BioAggregate showed higher cell viability than the first day extracts.…”

Section: Microscopy Resultsmentioning

confidence: 86%

“…[36] Our results show that MTA-derived materials could enhance the cell viability of 3T3 cells in vitro. Recent studies showed that the cytotoxic effects imposed by Ortho MTA, [26,27] Retro MTA, [37] BioAggregate, [18,28,29] Biodentine, [26,28,38,39] MTA Plus [40] and MTA Angelus exhibited negligible cytotoxicity. [41,42] While the extracts of BioAggregate promoted cell viability, Biodentine showed a reduced number of cells.…”

Section: Microscopy Resultsmentioning

confidence: 99%

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

Varol

Bahar

Karsli

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the effects of calcium silicate-based products on cytotoxicity in the 3T3 fibroblast and gelatinolytic activity of matrix metalloproteinases (MMPs). 3T3 fibroblasts were incubated directly with Ortho Mineral trioxide aggregate (MTA), BioAggregate, Biodentine, MTA Plus, MTA Angelus and MTA Cerkamed for 24 hours and seven days. The cytotoxicity was determined using an MTT assay. Supernatants were collected to determine MMP-2 and MMP-9. Data were analysed using IBM SPSS 22. Seventh day extracts of Ortho MTA and Biodentine showed reduced cell viability. Specific characterization of MMPs in cell culture demonstrated that MMP-2 (62 kPa) in the cell culture supernatants by gelatin zymography showed induced expression in four out of seven groups by 3T3 cells. No MMP-9 expression was observed. The cytotoxicity of materials revealed a significant difference in cell viability between the groups on the first and seventh days. The results of this study revealed minor cytotoxic effects for Ortho MTA and Biodentine. This study suggests that endodontic sealers induced production of MMP-2. MMP-9 might be expressed in small amounts when compared with MMP-2.

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“…21,29 A newly developed CSC, with zirconium oxide (ZrO 2 ), named Ortho-MTA (OMTA; BioMTA, Seoul, Korea) has promoted an inflammatory response and odontoblastic differentiation similar to Biodentine and MTA Angelus in human dental pulp cells (hDPCs). 30 Furthermore, CSC has been found to stimulate osteogenesis in a co-culture of macrophage cells (Raw 264.7) and osteoblast cells (MC3T3-E1), with improved proliferation and higher calcium deposition than cells in a monoculture. 31 In our recent study, the silicate-based cement associated with ZrO 2 exhibited a viability rate similar to that of MTA Plus and Biodentine in osteoblast-like cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage

et al. 2016

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Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage Abstract: Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials.

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Effects of Calcium Silicate Endodontic Cements on Biocompatibility and Mineralization-inducing Potentials in Human Dental Pulp Cells

Cited by 81 publications

References 44 publications

Tomographic Evaluation of Reparative Dentin Formation after Direct Pulp Capping with Ca(OH)2, MTA, Biodentine, and Dentin Bonding System in Human Teeth

Tomographic Evaluation of Reparative Dentin Formation after Direct Pulp Capping with Ca(OH)2, MTA, Biodentine, and Dentin Bonding System in Human Teeth

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage

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