Effects of combinations of Fusarium mycotoxins on the inhibition of macromolecular synthesis, malondialdehyde levels, DNA methylation and fragmentation, and viability in Caco-2 cells

Kouadio, James Halbin; Dano, Sébastien D.; Moukha, Serge; Mobio, Théophile A.; Creppy, E. E.

doi:10.1016/j.toxicon.2006.09.029

Cited by 172 publications

(99 citation statements)

References 55 publications

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“…The combination of the three toxins was not more effective in lipid peroxidation or in inhibiting DNA synthesis and methylation as compared to individual toxins, but the toxin combination was more effective than individual toxins in damaging DNA, suggesting an important aspect for further study. 30 We did not investigate the individual toxins in our study; antagonistic, additive, or synergistic effects of the combination of three toxins are possible. In light of these results, Grainsure E may be beneficial in reducing adverse effects of fumonisin B1 and deoxynivalenol in animals.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Qiang

Truong²,

Meynen³

et al. 2011

J. Agric. Food Chem.

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It was hypothesized that a mycotoxin binder, Grainsure E, would inhibit adverse effects of a mixture of fumonisin B1, deoxynivalenol, and zearalenone in rats. For 14 and 28 days, 8-10 Sprague-Dawley rats were fed control diet, Grainsure E (0.5%), toxins (7 μg fumonisin B1/g, 8 μg of deoxynivalenol/g and 0.2 μg of zearalenone/g), toxins (12 μg of fumonisin B1/g, 9 μg of deoxynivalenol/g, and 0.2 μg of zearalenone/g + Grainsure E), or pair-fed to control for food intake of toxin-fed rats. After 28 days, decreased body weight gain was prevented by Grainsure E in toxin-fed female rats, indicating partial protection against deoxynivalenol and fumonisin B1. Two effects of fumonisin B1 were partly prevented by Grainsure E in toxin-fed rats, increased plasma alanine transaminase (ALT) and urinary sphinganine/sphingosine, but sphinganine/sphingosine increase was not prevented in females at the latter time point. Grainsure E prevented some effects of fumonisin B1 and deoxynivalenol in rats. KeywordsAmes Laboratory, fumonisin; deoxynivalenol; zearalanone; mycotoxin binder; Grainsure E ABSTRACT: It was hypothesized that a mycotoxin binder, Grainsure E, would inhibit adverse effects of a mixture of fumonisin B1, deoxynivalenol, and zearalenone in rats. For 14 and 28 days, 8À10 SpragueÀDawley rats were fed control diet, Grainsure E (0.5%), toxins (7 μg fumonisin B1/g, 8 μg of deoxynivalenol/g and 0.2 μg of zearalenone/g), toxins (12 μg of fumonisin B1/g, 9 μg of deoxynivalenol/g, and 0.2 μg of zearalenone/g + Grainsure E), or pair-fed to control for food intake of toxin-fed rats. After 28 days, decreased body weight gain was prevented by Grainsure E in toxin-fed female rats, indicating partial protection against deoxynivalenol and fumonisin B1. Two effects of fumonisin B1 were partly prevented by Grainsure E in toxin-fed rats, increased plasma alanine transaminase (ALT) and urinary sphinganine/sphingosine, but sphinganine/sphingosine increase was not prevented in females at the latter time point. Grainsure E prevented some effects of fumonisin B1 and deoxynivalenol in rats.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Qiang

Truong²,

Meynen³

et al. 2011

J. Agric. Food Chem.

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“…The NR test was performed to assess cytotoxicity, as previously described by [7]. Viable cells actively transport this dye across their cell membrane; therefore, after subsequent lyses absorbance can be used as a measure of cell viability.…”

Section: Cytotoxicity Assay By Neutral Red (Nr) Testmentioning

confidence: 99%

“…The most significant mycotoxins in terms of public health and agronomic perspective include the aflatoxins, ochratoxin A (OTA), trichothecenes, fumonisins, zearalenone and patuline [3] and they were largely studied concerning their toxic aspects. The toxic effect of mycotoxin ingestion in both humans and animals depends on a number of factors including intake levels, the toxicity of the compound, duration of exposure (acute or chronic), the body weight of the individual, the presence of other mycotoxins (synergistic effects), mechanisms of action, metabolism, and defense mechanisms [4,1,5,6,7]. Aflatoxins B 1 (AFB 1 ) and ochratoxin A are mycotoxins abundantly produced in tropical areas such as Côte d'Ivoire.…”

Section: Introductionmentioning

confidence: 99%

“…For example, studies focused on mycotoxins presence in foods from Côte d'Ivoire reported co-occurrence of aflatoxin B 1 , ochratoxin A, fumonisin and zearalenone in rice, maize, peanuts [21]. Since, multi-exposure could lead to additive, synergistic or antagonistic effects [5,7,15,16], several combinations of mycotxins such as binary or tertiary combination were tested on cells line in the aim to observe their simultaneous effect on cellular damages namely cytotoxicty, genotoxicity, or cells metabolism [5,7,15,16] but very few had concerned the mixture of AFB 1 and OTA [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Low level of Ochratoxin A enhances Aflatoxin B1 Induced Cytotoxicity and Lipid Peroxydation in Both Human Intestinal (Caco-2) and Hepatoma (HepG2) Cells Lines

Halbin¹,

Brou²,

Dago³

2013

IJNFS

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Abstract:Aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) are contaminants which co-occurred in the same food such as cereals. The few studies performed on their interactive effect had revealed additive or antagonistic cytotoxic effect according to cells endpoints and concentrations of both mycotoxins. The aim of the present study was to investigate in a possible influence of very low level of ochratoxin A in aflatoxin B 1 toxic action regarding cellular endpoints such as malonedialdehyde (MDA) production and cells viability as evaluated by lysosome and mitochondria integrities and cell lactate dehydrogenase (LDH) leakage. OTA (20nM) and AFB 1 were tested in combination in both human intestinal (Caco-2) and hepatoma (HepG2) cells lines. As results, OTA alone tested at 20nM was not cytotoxic and did not induce MDA production in both Caco-2 and HepG2 cells line. Interestingly, combined to AFB 1 (10µM), OTA enhanced markedly AFB 1 cytotoxic effect. OTA significantly increased cell lysosomes damage induced by AFB 1 from 24% to 38% (+14%) and from 28% to 43% (+15%) respectively in Caco-2 and HepG2 cells line (p<0.05). Similarly, OTA enhanced inhibition of mitochondria succinate dehydrogenase activity induced by AFB 1 until to +15% and +6% respectively in Caco-2 and HepG2 cells line (p<0.05). On cell necrosis marker, the mixture of OTA and AFB 1 induced more LDH leakage when compared to AFB 1 alone with increase of +14% and +12% respectively in Caco-2 and HepG2 cells line (p<0.05). Finally, on MDA production, AFB 1 + OTA induced more intensively MDA production when compared to AFB 1 alone with +49% and +110% of increasing in both Caco-2 and HepG2 cells line (p<0.01). Taken together, our results suggested that combined AFB 1 and OTA induced all the toxicities observed with the mycotoxins separately but more intensively suggesting synergistic or potentiating effect. Moreover, AFB 1 or its association with OTA had been found very potent in human hepatic cells HepG2 in necrosis induction but especially in lipids oxidative damage confirming oxidative stress as one of keys pathways in toxic action of AFB 1 .

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“…DON could increase the production of free radicals, thus initiating oxidative stress [6][7][8][9], and both in vitro [10][11][12] and in vivo [13][14][15] studies have suggested that DON causes DNA fragmentation and lipid peroxidation (LPO), which lead to cell death and apoptosis through oxidative stress. On the other hand, previous research has found that antioxidants including epigallocatechin gallate (EGCG) [16], lutin [17], flavanols and vitamins E (α-tocopherol), A (β-carotene) or C (ascorbic acid) [18] could protect animals from DON toxicity.…”

Section: Introductionmentioning

confidence: 99%

Effect of Selenium Nanoparticles on Anti-Oxidative Level, Egg Production and Quality and Blood Parameter of Laying Hens Exposed to Deoxynivalenol

Qu¹,

Yang²,

Sun³

et al. 2017

J Anim Res Nutr

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The aim of this study was to investigate the efficacy of selenium nanoparticles (SeNPs) that had been biologically synthesized by Bacillus licheniformis to counteract deoxynivalenol (DON) toxicity in laying hens. Ninety-six healthy, 20-week-old laying hens were randomly assigned to four treatment groups, each of which included 3 replicates of 8 layers, and fed four different diets: an uncontaminated basal diet (control group), a 10 mg/kg DON-contaminated basal diet (DON group), a 10 mg/kg DON-contaminated basal diet with 0.5 mg/kg SeNPs (DON+SeNPs group) and the basal diet with 0.5 mg/kg SeNPs (SeNPs group).Serum T-AOC and GPx activities were significantly decreased (P<0.05) in hens fed the DON-contaminated diet, and DON significantly decreased the egg production rate (P<0.05), significantly increased the soft-shelled or cracked egg rate (P<0.05), significantly decreased serum calcium and inhibited the immune systems of the animals according to blood routine indexes. However, SeNPs improved the levels of GPx and T-AOC, increased the egg production rate, significantly decreased the soft-shelled or cracked egg rate (P<0.05), and decreased the influence of DON on the blood routine.In addition, SeNPs significantly (P<0.05) increased serum calcium. However, no differences were observed in egg quality (egg weight, Haugh units, yolk color, eggshell strength and eggshell thickness) among the four groups. These results showed that SeNPs could provide effective anti-oxidative protection against DON toxicity in laying hens, reduced DON's influence on egg production and blood calcium

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Effects of combinations of Fusarium mycotoxins on the inhibition of macromolecular synthesis, malondialdehyde levels, DNA methylation and fragmentation, and viability in Caco-2 cells

Cited by 172 publications

References 55 publications

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Low level of Ochratoxin A enhances Aflatoxin B1 Induced Cytotoxicity and Lipid Peroxydation in Both Human Intestinal (Caco-2) and Hepatoma (HepG2) Cells Lines

Effect of Selenium Nanoparticles on Anti-Oxidative Level, Egg Production and Quality and Blood Parameter of Laying Hens Exposed to Deoxynivalenol

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