Effects of CpG-DNA from Escherichia coli on Digoxin Pharmacokinetics

Abstract

Deoxyribonucleic acid (DNA) from bacteria or viruses has been reported as one of the pathogen-associated molecular patterns (PAMPs) and a substance that can induce endotoxemia-like inflammation in animals. However, there has been no report on digoxin pharmacokinetics in the inflammation induced by bacterial DNA containing unmethylated CpG motifs (CpG-DNA). In this study, we investigated the effects of CpG-DNA on digoxin pharmacokinetics. We determined the degree of lipopolysaccharide contamination in CpG-DNA solution and examined the changes in digoxin pharmacokinetics in rats after CpG-DNA administration. In addition, plasma concentrations of tumor necrosis factor-a a (TNF-a a), interleukin-1b b (IL-1b b), and nitrite/nitrate (NOx) were determined after CpG-DNA administration (5 mg/kg, i.p.). The AUC 0-24 of digoxin increased significantly on Day 1-3 and CL/F decreased on Day 1 and Day 2 after CpG-DNA administration. On Day 7 after CpG-DNA administration, there were no significant differences in AUC 0-24 and CL/F compared with the control group (without CpG-DNA administration). However, Kel remained relatively unchanged throughout the experiment. Plasma TNF-a a concentrations were significantly increased at 1 h and plasma IL-1b b concentrations were significantly decreased at 6 h after administration of CpG-DNA, while plasma NOx concentrations were significantly increased at 12 h after CpG-DNA administration, compared with the control group. These findings suggest that CpG-DNA (5 mg/kg) induces a transient inflammatory condition, and that AUC 0-24 and CL/F of digoxin were altered after CpG-DNA administration. Digoxin pharmacokinetics recovered within 7 d after CpG-DNA exposure.

Changes of midazolam pharmacokinetics in Wistar rats treated with lipopolysaccharide: relationship between total CYP and CYP3A2

“…[5][6][7] However, there are few reports about the recovery process on drug pharmacokinetics during infection. [8][9][10] In this study, we investigated the effects of LPS on total cytochrome P450 (CYP), CYP3A2, and CYP2C11 contents in the liver, and measured tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and nitrite/nitrate (NOx) concentrations in plasma in a transient LPS-induced endotoxemia model of rats that were able to recover. 8 In addition, to assess the effects of LPS on CYP3A2 activities, the pharmacokinetics of midazolam, 11,12 a CYP3A2 substrate, were investigated.…”