Effects of CSN3 and LGB gene polymorphisms on production traits in beef cattle

Curi, Rogério Abdallah; Oliveira, Henrique Nunes de; Gimenes, Marcos Aparecido; Silveira, Antônio Carlos; Lopes, Catalina Romero

doi:10.1590/s1415-47572005000200015

Cited by 25 publications

(17 citation statements)

References 15 publications

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“…In contrast to the previously mentioned results, the authors reported that the AA genotype of K-CN gene were characterized by the highest milk, fat and protein yield, while the lowest fat and protein contents were observed in milk of cows with the BB genotype. This association between AA genotype with higher milk production agreed with the results of Curi et al [13].…”

Section: Kappa-caseinsupporting

confidence: 92%

Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo

Othman

Zayed

Gawead

et al. 2011

Journal of Genetic Engineering and Biotechnology

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In dairy farm animals, the principal goal of the selection is the improvement of milk yield and composition. The genes of milk proteins and hormones are excellent candidate genes for linkage analysis with quantitative trait loci (QTL) because of their biological significance on the quantitative traits of interest.Prolactin (PRL) is a polypeptide hormone with multiple functions, secreted mainly by the anterior pituitary gland. Prolactin's biological activity consists of various roles in the reproduction, lactation and a number of homeostatic biological functions including immune functions.Casein proteins and their genetic variants have been reported as important factors associated with lactation performance, milk composition and cheese yield efficiency. Genetic variants of bovine kappa-casein (K-CN) gene are associated with milk protein content and have a significant influence on rennet clotting time, firmness and cheese yield of milk.The pituitary-specific transcription factor (PIT-1) gene is responsible for pituitary development and hormone secreting gene expression in mammals. PIT-1 is studied as a candidate genetic marker for growth, carcass and also for milk yield traits.Genomic DNA extracted from 100 healthy buffaloes was amplified using primers that were designed from the cattle PRL, K-CN and PIT-1 gene sequences. The amplified fragments of PRL (294-bp), K-CN (530-bp) and PIT-1 (451-bp) were digested with RsaI, HindIII and HinfI restriction enzymes, respectively. The results showed that all tested buffaloes are genotyped as GG for PRL, BB for K-CN and BB for PIT-1.

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Section: Kappa-caseinsupporting

confidence: 92%

Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo

Othman

Zayed

Gawead

et al. 2011

Journal of Genetic Engineering and Biotechnology

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“…However, Biase et al 2005 indicate that a kappa-casein polymorphism did not influence weight-gain in Brazilian Nellore herds, although they found differences in the frequency of the A and B alleles, with the A allele observed most frequently in this beef breed. Similar results were reported by Curi et al 2005 in Nellore, Canchim and a crossbreed (Simmental x Nellore, Angus x Nellore) cattle. Here, we include animals from the Charolais breed, and we found frequencies similar to those reported by Jann et al (2004).…”

Section: Fig 2 Confirmation Of Pcr-rflp For Variants E and H Of K-casupporting

confidence: 89%

Convenient genotyping of nine bovine K-casein variants

Contreras¹,

Jaramillo²,

Parra‐Bracamonte³

et al. 2011

Electro Journal of Biotech

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K-casein gene polymorphisms are of major importance in the dairy industry due to their association with different quality and productive traits (i.e., milk protein). Several methods for genotyping this gene have been proposed; however, none are focused on the simultaneous discrimination of nine K-casein variants. A strategy based on PCR-RFLP was designed to characterize nine K-casein variants (A, B, C, E, F 2 , G, H, I and J) and used to genotype three cattle populations: Gyrholando (3/8 Gyr x 5/8 Holstein), Charolais and Carora. The B variant was the most frequent in the Charolais and Carora breeds, with allelic frequencies of 0.60 and 0.59, respectively. In the Gyrholando breed, four variants were found, with the A variant being the most frequent and E and H the least. The genotyping strategy was effective in detection and differentiation of K-casein variants, and it is proposed for use in laboratories with minimal molecular biology equipment for genotyping and evaluation of the phenotypic effects of nine K-casein variants on milk production and quality.

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“…Alison Van Eenennaam and Medrano (1991) compared κ-casein AA and BB genotypes and showed that the BB genotype showed first lactational yields increased by 296 kg with regard to AA. In contrast, Gonyon et al (1987) and Curi et al (2005) reported that the κ-casein genotype AA was associated with higher milk production than BB, with the heterozygous AB being intermediate, whereas Ng-Kwai-Hang et al (1986) and S Kim, M.Sc. Thesis, McGill University, Montreal, QC, 1994) showed that κ-casein AB animals were better milk producers than either of the homozygous animals, in Ayrshire, Holstein, Jersey, brown Swiss, Canadienne and Guernsey cattle breeds.…”

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confidence: 93%

Bovine kappa-casein gene polymorphism and its association with milk production traits

Rachagani

Gupta

2008

Genet. Mol. Biol.

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Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism was detected by digestion of PCR-amplified products with HindIII, HhaI and HaeIII restriction enzymes, followed by separation on 3% agarose gels, and resolved by ethidium bromide staining. Allele A of the κ-casein gene occurred at a higher frequency than allele B, in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in the Sahiwal and Tharparkar breeds were 0.758, 0.230 and 0.012, and 0.0.732, 0.250 and 0.018, respectively. The frequencies of alleles A and B in the Sahiwal and Tharparkar breeds were 0.873 and 0.127, and 0.857 and 0.143, respectively. Genotype BB of the kappa-casein gene had more influence on the monthly milk yield, 305-days milk yield, monthly solids-not-fat (SNF) yield, and monthly protein yield, in the Sahiwal cattle.

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Effects of CSN3 and LGB gene polymorphisms on production traits in beef cattle

Cited by 25 publications

References 15 publications

Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo

Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo

Convenient genotyping of nine bovine K-casein variants

Bovine kappa-casein gene polymorphism and its association with milk production traits

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