Effects of dexamethasone, vitamin A and vitamin D3 on DSP-PP mRNA expression in rat tooth organ culture

Ritchie, Helena H.; Park, H.; Liu, J.; Bervoets, T.J.M.; Bronckers, A.L.J.J.

doi:10.1016/j.bbaexp.2004.07.004

Cited by 14 publications

(8 citation statements)

References 30 publications

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“…It has been demonstrated that dexamethasone (Dex) and/or β-glycerophosphate (β-GP) in the culture medium of dental pulp cells can induce odontoblast features (51)(52)(53)(54)(55)(56)(57)(58)(59)(60). Cultured dental pulp cells were spindle-shaped in appearance with extended cytoplasmic processes and were grown to a near-confluent state.…”

Section: Resultsmentioning

confidence: 98%

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Huang

Xu²,

Gao³

et al. 2011

Int J Mol Med

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Abstract. Dentin sialophosphoprotein (DSPP), an important marker of odontoblast differentiation, is a prerequisite for tooth development and mineralization; however, the molecular mechanisms of both temporal and spatial regulation remain unknown. MicroRNAs (miRNAs) provide an additional level of control beyond that of transcription factors, which regulate post-transcriptional control of gene expression. The present study was designed to provide a first attempt at an in-depth analysis of dental pulp cells at various odontoblastic differentiation stages to obtain miRNA differential expression patterns, and to determine the contribution of miRNAs in the expression of DSPP during odontoblast differentiation. Dual luciferase reporter assays and qRT-PCR were used to validate miRNAs identified from bioinformatic analyses to determine whether they were able to regulate the DSPP gene in dental pulp cells cultured in a mineralizing medium. The results presented here suggest that DSPP is regulated post-transcriptionally by mir32, mir885-5p and mir586 during odontoblast differentiation.

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Section: Resultsmentioning

confidence: 98%

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Huang

Xu²,

Gao³

et al. 2011

Int J Mol Med

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“…The main proteins engaged in tooth biomineralization have been studied with respect to their responsiveness to vitamin D. Most of these proteins appeared to be sensitive to vitamin D status. In dentin‐forming cells, osteocalcin (27) and osteopontin (28) levels were shown to be modulated by vitamin D, albeit at various degrees, while dentine sialophosphoprotein expression was not (13,28). In enamel‐forming cells, calbindins (9), enamelin (13), ameloblastin/amelin/sheathlin (13), and amelogenin (29) were found to be either positively or negatively controlled by vitamin D.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D and tissue non‐specific alkaline phosphatase in dental cells

Lezot

Descroix

Hotton

et al. 2006

European J Oral Sciences

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Dental epithelium comprises different cell populations, including ameloblasts and stratum intermedium cells. Ameloblasts are vitamin D targets, and at least five proteins undergo specific modulation of their expression following the addition of 1alpha,25(OH)2 vitamin D3[1alpha,25(OH)2D3]. Stratum intermedium cells have not been studied in any great detail regarding vitamin D impact. Interestingly, in these cells, the tissue non-specific alkaline phosphatase (TNAP) is overexpressed. On the other hand, TNAP is a reliable bone marker of vitamin D action, similar to calbindins in kidney and intestine, previously used for studies of vitamin D activity in ameloblasts. Here, TNAP expression and activity were investigated in vivo in the microdissected epithelium and mesenchyme of mandible incisors. Physiological doses of 1alpha,25(OH)2D3 injected in control rats failed to modify TNAP activity in both dental epithelium and mesenchyme. No significant differences were observed in the steady-state levels of TNAP mRNAs of dental tissues from wild-type and vitamin D nuclear receptor (VDRnuc)-deficient mice of the same litters. These data suggest that, in contrast to ameloblasts, stratum intermedium cells are not sensitive to 1alpha,25(OH)2D3. An explanation for such a responsiveness of stratum intermedium cells to 1alpha,25(OH)2D3 is proposed based on the respective expressions of both vitamin D receptors (VDRnuc and 1,25D3-[MARRS]) and the Dlx2 homeobox gene.

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“…On the other hand, Dex, which is popularly used to induce osteoblast differentiation (26), downregulated odontoblast differentiation in this study. Dex (10 −8 M) induces expression of Dspp in human dental pulp cells (27), and cultured rat tooth organ (28). However, Dex (10 −7 M) upregulated the expressions of ALP and type I collagen in MDPC‐23 cells (23).…”

Section: Discussionmentioning

confidence: 99%

Expression of Notch signalling‐related genes in normal and differentiating rat dental pulp cells

Sun

Kawashima

et al. 2010

Aust Endodontic J

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Notch signalling is of fundamental importance to various processes during embryonic development and in adults. The possible role of Hey1, an important Notch signalling component, in odontoblast differentiation was evaluated in this study. Primary cultured dental pulp cells, derived from upper incisors of 5-week-old Wistar rats, were placed in alpha-modification of Eagle's minimal essential medium supplemented with 10% Fetal Bovine Serum (FBS), and ascorbic acid (AA) and beta-glycerophosphate (beta-GP), with or without dexamethasone, and cultured on dishes coated with collagen type IA for 7 days. Conventional and real-time Polymerase Chain Reaction (PCR) was performed to determine the expression of Notch-related genes and dentin sialophosphoprotein as a marker of odontoblast differentiation. Dentin sialophosphoprotein and Hey1 expression was significantly increased and decreased in the presence of AA + beta-GP compared with controls, respectively. These findings suggest that Hey1 may be a negative regulator in odontoblast differentiation.

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Effects of dexamethasone, vitamin A and vitamin D3 on DSP-PP mRNA expression in rat tooth organ culture

Cited by 14 publications

References 30 publications

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Vitamin D and tissue non‐specific alkaline phosphatase in dental cells

Expression of Notch signalling‐related genes in normal and differentiating rat dental pulp cells

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