Effects of dietary LNA/LA ratios on growth performance, fatty acid composition and expression levels of elovl5, Δ4 fad and Δ6/Δ5 fad in the marine teleost Siganus canaliculatus

Xie, Dizhi; Liu, Xuebing; Wang, Shuqi; You, Cuihong; Li, Yuanyou

doi:10.1016/j.aquaculture.2017.08.039

Cited by 15 publications

(15 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest level of Δ6 fad activity was reported at dietary ALA:LA ratios of 1.54 in Murray cod, whereas in rainbow trout it was 2.63 . The expression of Δ4 and Δ6/Δ5 fad and elovl5 mRNA in the liver of rabbitfish was influenced by dietary ALA:LA ratios and the highest expression reported at a dietary ALA:LA ratio of 1.93 . Similarly, in butterfish, elovl5 and elovl4 mRNA expression in liver was also influenced by dietary ALA:LA ratios and the highest expression occurred at an ALA:LA ratio of 1.7 .…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

et al. 2019

View full text Add to dashboard Cite

Effects of dietary -linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and 6 fad and elovl5 gene expression in silver barb (Puntius gonionotus) Abstract BACKGROUND: Silver barb (Puntius gonionotus) is a medium-sized carp that is promising for freshwater aquaculture in Asia. This study's aim was to investigate the ideal dietary -linolenic acid (ALA): linoleic acid (LA) ratio for maximizing long-chain polyunsaturated fatty acid (LC-PUFA) synthesis and their deposition in the muscle of silver barb, as that of fish oil based control diet.RESULT: Fish (with an initial body weight of 11.07 ± 0.12 g) were fed for 60 days with five experimental iso-proteinous, iso-lipidic, and iso-caloric diets, supplemented with linseed oil and peanut oil at varying levels to obtain ALA:LA ratios of 0.35, 0.51, 0.91, 2.04, 2.66. A control diet was prepared by supplementing fish oil. The dietary ALA:LA ratio did not influence the growth performance of fish. With increased dietary ALA:LA ratios, LA content decreased and ALA content increased in the muscle and liver of silver barb. The n-3 LC-PUFA level in muscle and liver was not influenced by feeding different ratios of ALA:LA, whereas n-6 LC-PUFA was decreased in the muscle and increased in the liver with increased dietary ALA:LA ratios. Increasing dietary ALA:LA ratio increased the 6fad and elovl5mRNA expression in the liver, muscle, brain, and intestinal tissues of silver barbs. CONCLUSION: Silver barb possess the ability to elongate and desaturate ALA and LA to their end products EPA and DHA. The highest level expression of 6 fad and elovl5 mRNA at the dietary ALA:LA ratio of 2.66 suggests greater affinity of these enzymes towards ALA than LA in silver barb.

show abstract

Section: Discussionmentioning

confidence: 89%

“…Therefore, a exact ratio of ALA/LA in the diets is essential to determine the fillet quality of the fish. Previous studies in different fish species indicated that dietary ALA:LA ratios influenced their growth, tissue fatty acid profile, fatty acid metabolism, immunological response, and reproduction …”

Section: Introductionmentioning

confidence: 99%

Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…It is indicated that water salinity might affect the LC-PUFA bioconversion capacity from C18 precursors by regulating the expression of desaturases and elongases in fish. Moreover, the optimal dietary ALA:LA ratio of 1·93 is proposed for S. canaliculatus juveniles ( 21 ) . However, the effect of environmental salinity on endogenous LC-PUFA biosynthesis in fish varies among different species.…”

mentioning

confidence: 99%

Comparison of the growth performance and long-chain PUFA biosynthetic ability of the genetically improved farmed tilapia (Oreochromis niloticus) reared in different salinities

You

Wang

et al. 2019

Br J Nutr

Self Cite

View full text Add to dashboard Cite

To compare the growth and biosynthetic ability of long-chain PUFA (LC-PUFA) of the genetically improved farmed tilapia (GIFT) (Oreochromis niloticus) in different water salinities, an 8-week feeding trial was conducted on the GIFT juveniles at 0, 12 and 24 ‰ (parts per thousand; ppt), respectively, with three isonitrogenous (32 %) and isolipidic (8 %) diets (D1–D3). Diet D1 with fish oils (rich in LC-PUFA) as lipid source was used as the control, while D2 and D3 with vegetable oil (free LC-PUFA) blends as lipid source contained different ratios of linoleic acid (LA, 18 : 2n-6) and α-linolenic acid (ALA, 18 : 3n-3) at 4·04 (D2) and 0·54 (D3), respectively. At the end of feeding trial, the growth performance of D2 and D3 groups under all salinity treatments was as good as that of D1 group, which indicates that the GIFT juveniles may convert dietary LA and ALA into LC-PUFA to meet the requirement of essential fatty acids for normal growth and physiology. When fed the same diets, GIFT at 12 ppt had a better growth performance coupled with a higher liver and muscle arachidonic acid content than those in freshwater. Furthermore, brackish water (24 ppt) significantly promoted the mRNA levels of elongase 5 of very long-chain fatty acids (elovl5) and peroxisome proliferator-activated receptor α (pparα) in liver, when compared with freshwater. These results suggest that the GIFT may display better growth performance together with a relatively higher endogenous LC-PUFA biosynthetic ability under brackish water (12 and 24 ppt), probably through improving the expression of elovl5 and pparα in liver.

show abstract

“…While this is not something that had been highlighted previously, careful examination of existing literature has shown that increased dietary LA (i.e. reduced ALA:LA ratio) generally resulted in increased DHA:EPA ratios in whole fish and/or tissues of a range of freshwater and salmonid species (68,73,(75)(76)(77)(78)(79)(80)(81) , though not always (72,82,83) . It must be stressed that the above results are not directly comparable with the present study as the fish in these earlier trials were all fed diets that also contained EPA and DHA, complicating interpretation of the data.…”

Section: Discussionmentioning

confidence: 77%

“…Similarly, there was no effect of dietary ALA:LA ratio on the expression of Δ6 desaturase and elovl5 in liver of rainbow trout (68) . Dietary ALA:LA ratio affected the expression of Δ6 and Δ4 desaturases and elovl5 in the marine rabbit fish (Siganus canaliculatus), which has all the genes necessary for biosynthesis of EPA and DHA, but in a variable manner with no clear pattern (81) . Interestingly, in the present study, brain showed relatively high contents of EPA in addition to DHA, which may indicate in situ production of DHA in brain.…”

Section: Discussionmentioning

confidence: 99%

Endogenous production ofn-3 long-chain PUFA from first feeding and the influence of dietary linoleic acid and theα-linolenic:linoleic ratio in Atlantic salmon (Salmo salar)

Sprague

Betancor

et al. 2019

Br J Nutr

View full text Add to dashboard Cite

Atlantic salmon (Salmo salar) possess enzymes required for the endogenous biosynthesis of n-3 longchain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from a-linolenic acid (ALA). Linoleic acid (LA) competes with ALA for LC-PUFA biosynthesis enzymes leading to the production of n-6 LC-PUFA, including arachidonic acid (ARA).We aimed to quantify the endogenous production of EPA and DHA from ALA in salmon fed from first feeding on diets that contain no EPA and DHA, and to determine the influence of dietary LA and ALA:LA ratio on LC-PUFA production. Salmon were fed from first feeding for 22 weeks with three diets formulated with linseed and sunflower oils to provide ALA:LA ratios of approximately 3:1, 1:1 and 1:3. Endogenous production of n-3 LC-PUFA was 5.9, 4.4 and 2.8 mg per g fish and that of n-6 LC-PUFA was 0.2, 0.5 and 1.4 mg per g of fish in salmon fed diets with ALA:LA ratios of 3:1, 1:1 and 1:3, respectively. The ratio of n-3:n-6 LC-PUFA production decreased from 27.4 to 2.0, and DHA:EPA ratio increased and EPA:ARA and DHA:ARA ratios decreased, as dietary ALA:LA ratio decreased. In conclusion, with a dietary ALA:LA ratio of 1, salmon fry/parr produced around 28 µg n-3 LC-PUFA per g of fish per day, with a DHA:EPA ratio of 3.4. Production of n-3 LC-PUFA exceeded that of n-6 LC-PUFA by almost 9-fold. Reducing the dietary ALA:LA ratio reduced n-3

show abstract

Effects of dietary LNA/LA ratios on growth performance, fatty acid composition and expression levels of elovl5, Δ4 fad and Δ6/Δ5 fad in the marine teleost Siganus canaliculatus

Cited by 15 publications

References 45 publications

Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

Comparison of the growth performance and long-chain PUFA biosynthetic ability of the genetically improved farmed tilapia (Oreochromis niloticus) reared in different salinities

Endogenous production ofn-3 long-chain PUFA from first feeding and the influence of dietary linoleic acid and theα-linolenic:linoleic ratio in Atlantic salmon (Salmo salar)

Contact Info

Product

Resources

About