Effects of dietary sulphur sources on concentrations of hydrogen sulphide in the rumen head-space gas of dairy cows

Dewhurst, Richard J.; Kim, E.J.; Evans, R.T.; Cabrita, Ana R.J.; Fonseca, António J.M.

doi:10.1017/s1751731107705311

Cited by 6 publications

(7 citation statements)

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“…Methionine in SBM was much less degradable than in the other protein sources (MGF and SFM). The degradability of methionine was always lower than cystine, confirming the results of Dewhurst et al (2007). The batches of MGF differing in colour also differed in S degradability, as well in concentrations of hydrogen sulphide in rumen headspace gas.…”

Section: Protein Sourcessupporting

confidence: 79%

“…Concentration of hydrogen sulphide in the rumen headspace gas Dewhurst et al (2007) confirmed that cyst(e)ine is the dominant source of hydrogen sulphide in rumen gas and showed that there was a linear increase in hydrogen sulphide concentrations in response to increasing cysteine additions. In Expt 1, the concentration of hydrogen sulphide was very different between protein sources, with MGF having the highest concentration followed by SFM and SBM leading to a very low concentration.…”

Section: Protein Sourcesmentioning

confidence: 60%

“…Samples of feeds and protein sources were taken each day and analysed for DM (oven drying), ash (furnace), CP (microKjeldahl with Se catalyst), ether extract (extraction with petroleum ether using Gerhardt Soxtherm 2000 Automatic equipment, Foss, Hillerød, Denmark), NDF, ADF and ADL (van Soest detergent methodology with amylase and without sodium sulphite; using Fibretec equipment, Foss) and starch (enzymatic) according to Cabrita et al (2011a). AAs were analyzed on dried samples after initial oxidation with performic acid according to Dewhurst et al (2007). Fonseca, Cabrita, Pinho, Kim and Dewhurst of heat damage evaluated through their colour by visual observation (from lighter to darker: MGF1, MGF2, MGF3 and MGF4).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, studies showed cysteine to be the dominant source of hydrogen sulphide in the rumen, leading to concentrations higher than those for methionine and inorganic S sources (Bird, 1972;Dewhurst et al, 2007). However, the variable effect of cysteine on hydrogen sulphide concentrations noted in earlier work (Dewhurst et al, 2007) questions the accuracy of the proposed approach as an assay for protein degradation.…”

Section: Introductionmentioning

confidence: 98%

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Effects of protein sources on concentrations of hydrogen sulphide in the rumen headspace gas of dairy cows

Fonseca

Cabrita

Pinho

et al. 2013

Animal

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Two Latin square design experiments investigated the relationship between hydrogen sulphide concentration in the rumen headspace gas of dairy cows and the early stages of protein degradation in the rumen. In Expt 1, three protein sources differing in rumen N (nitrogen) degradability (maize gluten feed (MGF); sunflower meal (SFM); and soyabean meal (SBM)) were used, whereas in Expt 2 four different batches of the same feed (MGF) differing in colour (CIE L*, a*, b* (CIELAB) scale) were used. After allowing the concentration of hydrogen sulphide in rumen gas to decline close to zero, a fixed amount of protein sources was offered to cows and the concentrations of hydrogen sulphide were recorded in rumen headspace gas at 30-min intervals. In Expt 1, the concentration of hydrogen sulphide showed considerable variation between protein sources, with MGF having the highest concentration followed by SFM and SBM resulting in very low concentrations. The N wash losses (zero time measurements with nylon bags) ranked the feeds in the same way, from MGF (highest; 61%) to SBM (lowest; 26%). There were marked differences in the degradation of cystine and methionine between protein sources, although the degradation of cystine was always higher than for methionine. MGF (Expt 2) led to increased concentrations of hydrogen sulphide, with peak concentrations achieved between 1 and 2 h after feeding. The concentrations of hydrogen sulphide were higher for MGF1, intermediate for MGF2 and lower for MGF3 and MGF4, agreeing with colour scale. Differences in the early stages of dietary sulphur degradation corresponded with differences in hydrogen sulphide concentrations in rumen gas. The results suggest that hydrogen sulphide concentrations in the rumen headspace gas could be useful to evaluate nutritional parameters not measured by the in sacco technique, contributing to a better understanding of the response of dairy cows to different protein supplements.Keywords: dairy cow, hydrogen sulphide, rumen, protein sources Implications Differences in rumen headspace gas concentrations of hydrogen sulphide are related to the degradation of sulphur (S) in the rumen. As a high proportion of dietary S is contained within protein, this association offers potential for a new approach to compare protein degradation of different protein sources. This may be particularly useful for studying the early stages of degradation of soluble proteins where the existing nylon bag technique is of limited value.

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Section: Protein Sourcessupporting

confidence: 79%

Section: Protein Sourcesmentioning

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Effects of protein sources on concentrations of hydrogen sulphide in the rumen headspace gas of dairy cows

Fonseca

Cabrita

Pinho

et al. 2013

Animal

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“…1987, Gooneratne et al 1989, Sager et al 1990, Gould et al 1991, Hamlen et al 1993, Jeffrey et al 1994, McAllister et al 1997, Loneragan et al 1998, Gould 1998, Gould 2000, Niles et al 2000, Loneragan et al 2001, Niles et al 2002, Haydock 2003, Loneragan et al 2005, Kul et al 2006, Dewhurst et al 2007, McKenzie et al 2009). Outras etiologias descritas foram deficiência da tiamina (Jensen et al 1956, Edwin & Jackman 1973, intoxicação por cloreto de sódio associada à privação de água (Trueman & Clague 1978, Osweiler et al 1995, intoxicação por chumbo (Christian & Tryphonas 1971), ingestão de melaço (Mella et al 1976) ou de plantas ricas em tiaminases (Pritchard & Eggleston 1978) e infecção por herpesvírus bovino 5 (BoHV-5) (Perez et al 2003, Rissi et al 2008.…”

Section: Introductionunclassified

Polioencefalomalacia experimental em bovinos induzida por toxicose por enxofre

et al. 2011

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RESUMO.-O presente trabalho teve como objetivos avaliar os sinais clínicos, as concentrações do sulfeto de hidrogênio ruminal e as alterações anatomopatológicas associadas à intoxicação experimental por enxofre em bovinos. Foram utilizados dez bezerros mestiços leiteiros, sendo que quatro bovinos ingeriram ração sem sulfato de sódio (G1) e seis consumiram ração com sulfato de sódio (G2). Exames clínicos (temperatura retal, frequência cardíaca e respiratória e motricidade ruminal) e laboratoriais (hemograma, fibrinogênio, proteína plasmática, pH do fluido ruminal, concentração do sulfeto de hidrogênio ruminal, lí-quido cerebrospinal e histopatológico) foram realizados. A temperatura retal, frequência cardíaca, hemograma, fibrinogênio, proteína plasmática, pH do fluido ruminal e os valores do líquido cerebrospinal estavam dentro dos valores de referência para a espécie. Taquipnéia, hipomotricidade ruminal e elevados valores de sulfeto de hidrogênio ruminal foram observados nos bezerros do grupo G2. Um bezerro do grupo G2 apresentou sinais neurológicos e lesões histopatológicas de PEM. The aims of this study were to evaluate the clinical signs, the ruminal hydrogen sulfide concentration and the histological lesions induced by sulfur toxicosis in cattle. Ten crossbred calves were fed an experimental diet, four without sodium sulfate (G1) and six with (G2). The calves were submitted to clinical (rectal temperature, cardiac and respiratory rate and ruminal motricity) and laboratorial (hemogram, fibrinogen, total plasma protein, ruminal fluid pH, ruminal hydrogen sulfide concentration, cerebrospinal fluid and histopathological) evaluations. Rectal temperature, cardiac rate, hemogram, fibrinogen, total plasma protein, ruminal fluid pH and cerebrospinal fluid values were within normal reference ranges in animals from both groups. Ruminal hypomotricity and increased respiratory rate and ruminal hydrogen sulfide concentration occurred in G2 animals. One out of six calves in G2 developed neurological signs and lesions of PEM. Two calves of each Group were euthanized. Microscopic lesions of PEM were observed in G2 animals. Histologically there were cortical neuronal necrosis and hemorrhagic lesions in basal nuclei, thalamus, midbrain, pons and medulla oblongata. The experimental model consisting of a diet with high carbohydrate and low in long fiber content with high sulfur concentrations (0.52%) resulted in clinical and histological abnormalities and high ruminal hydrogen sulfide concentration consistent with sulpur toxicosis in cattle.INDEX-TERMS: Cerebrocortical necrosis, sulfur, cattle, experimental model, ruminal hydrogen sulfide.

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Review: Markers and proxies to monitor ruminal function and feed efficiency in young ruminants

Cheng

Cantalapiedra-Hijar

Meale

et al. 2021

Animal

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Effects of dietary sulphur sources on concentrations of hydrogen sulphide in the rumen head-space gas of dairy cows

Cited by 6 publications

References 15 publications

Effects of protein sources on concentrations of hydrogen sulphide in the rumen headspace gas of dairy cows

Effects of protein sources on concentrations of hydrogen sulphide in the rumen headspace gas of dairy cows

Polioencefalomalacia experimental em bovinos induzida por toxicose por enxofre

Review: Markers and proxies to monitor ruminal function and feed efficiency in young ruminants

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