Effects of dietary supplementation of vitamin-E and commercial probiotics on the innate immunity of Labeo rohita against Aeromonas hydrophila infection

Priyadarsani, Leesa; Abraham, Thangapalam Jawahar; Adikesavalu, Harresh; Dash, Gadadhar; Nagesh, T. S.

doi:10.1016/j.fsirep.2021.100013

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…Our results showed the significant enhancement of antiprotease activity in OFO+C500+E400 group compared to OFO group indicating enhanced protection of fish against invading pathogens (Shin et al, 2014). Likewise, Priyadarsani et al (2021) reported elevated serum antiprotease activity by VE supplementation in diets for L. rohita. However, no effect of VC was found on plasma antiprotease activity in hybrid striped bass (Morone chrysops × M. saxatilis) (Yamamoto et al, 2020).…”

Section: Discussionsupporting

confidence: 57%

Effects of Vitamin C and E Supplementation on Growth, Fatty Acid Composition, Innate Immunity, and Antioxidant Capacity of Rainbow Trout (Oncorhynchus mykiss) Fed Oxidized Fish Oil

et al. 2021

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This 10-week feeding experiment examined the effects of supplementing vitamin C (VC) and E (VE) in oxidized oil diets on growth, fatty acid composition, blood physiological indicators, innate immunity, antioxidant capacity, and liver and intestine histology of rainbow trout (Oncorhynchus mykiss). Four diets were produced including a fresh fish oil containing diet (FFO diet), an oxidized fish oil containing diet (OFO diet), and OFO diet supplemented with 500 mg kg–1 VC and 400 mg kg–1 VE (OFO+C500+E400 diet) or 1,000 mg kg–1 VC and 800 mg kg–1 VE (OFO+C1000+E800 diet). Four hundred and twenty fish averaging 46.9 ± 0.32 g were stocked into 12 tanks and fed the diets twice a day to visual satiety. The results showed no significant effect of OFO or vitamins supplementation on growth, feed intake and feed utilization (P > 0.05). The groups that received OFO and OFO+C1000+E800 diets had significantly (P < 0.05) lower hepatosomatic indices than the other groups. Muscle fatty acid composition showed decreased proportion of 15:0, 16:0, 16:1n-7, 16:2n-4, 18:1n-5, 18:2n-6 (linoleic acid) and total saturated fatty acids in OFO group. Whereas higher percentages of 20:3n-6 and 20:3n-3 were found in OFO group. In addition, the highest ratio of docosahexaenoic acid (DHA, 22:6n-3) to eicosapentaenoic acid (EPA, 20:5n-3) was detected in OFO+C500+E400 group. The highest serum triglyceride concentration was recorded in the OFO group. Serum aspartate aminotransferase activity increased in OFO and OFO+C1000+E800 groups compared to OFO+C500+E400 group. Furthermore, significantly higher alkaline phosphatase activity in blood was found in OFO and OFO+C500+E400 groups. Significantly lower serum lysozyme, antiprotease, superoxide dismutase, and catalase activities were recorded in OFO group compared to FFO and OFO+C500+E400 groups, and an opposite trend was observed for malondialdehyde concentration. Muscle VC and VE concentrations, and liver and intestine histology remained unaffected. To conclude, feeding diet containing oxidized oil with peroxide value of 182 meq kg–1 with/without VC and VE supplementation did not influence growth and tissue VC and VE concentrations of rainbow trout. However, supplementing 500 mg kg–1 VC and 400 mg kg–1 VE reversed the adverse effects of OFO on non-specific immune response and antioxidant capacity.

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Section: Discussionsupporting

confidence: 57%

Effects of Vitamin C and E Supplementation on Growth, Fatty Acid Composition, Innate Immunity, and Antioxidant Capacity of Rainbow Trout (Oncorhynchus mykiss) Fed Oxidized Fish Oil

et al. 2021

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“…MB2 showed the presence of α‐tocopherol, 1‐(+)‐ascorbic acid 2,6‐dihexadecanoate and 1‐heptatriacotanol; whereas EW1 showed the presence of Hexadecanoic acid, Phytol and cis, cis, cis‐7,10,13‐Hexadecatrienal (Table 6). Dietary supplementation of the potent antioxidant α‐tocopherol was reported to enhance resistance and immunity of Labeo rohita against A. hydrophila infection (Priyadarsani et al, 2021). Black seed oil containing 1‐(+)‐ascorbic acid 2,6‐dihexadecanoate as the major phytoconstituent was found to show antimicrobial properties against different bacterial and fungal strains (Ashraf et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…MB2 showed the presence of α-tocopherol, 1-(+)-ascorbic acid 2,6-dihexadecanoate and 1-heptatriacotanol; whereas EW1 showed the presence of Hexadecanoic acid, Phytol and cis, cis, cis-7,10,13-Hexadecatrienal (Table 6). Dietary supplementation of the potent antioxidant αtocopherol was reported to enhance resistance and immunity of Labeo rohita against A. hydrophila infection (Priyadarsani et al, 2021).…”

Section: Moustafa Et Al (2020) Reported That Intra-peritoneal Injecti...mentioning

confidence: 99%

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

Mukherjee

Ghosal

Marik

et al. 2022

Aquaculture Research

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The present study aimed to identify the antibacterial bioactive compounds in Withania roots and Basella leaves against Aeromonas infection in Nile tilapia. Withania ethanol extract (EW, ZoI 10.72 ± 1.028 mm) and Basella methanol extract (MB, ZoI 10.83 ± 1.058 mm) showed the highest antibacterial activity in disc diffusion assay.In in vivo experiment, Nile tilapia (25.61 ± 0.31 g) were fed basal, EW and MB (both at 0.7 g/kg feed) diets for 15 days either before being intraperitoneally injected with Aeromonas and basal diet feeding for 7 days (prophylactic) or after bacterial injection and basal diet feeding for 7 days (therapeutic). Fish fed EW and MB fortified diets showed significantly (p < 0.05) lower mortality, alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvate transaminase activities, HSP70, Caspase3, IFN-ɣ, IL-1 β, TNFα level, while significantly (p < 0.05) higher weight gain, protein efficiency ratio, respiratory burst, lysozyme, phagocytotic activity, plasma total protein, total immunoglobulin content, RBC, WBC count, haemoglobin, haematocrit, mean corpuscular haemoglobin concentration, mean corpuscular volume, lymphocytes, neutrophils, monocytes percentages and superoxide dismutase, catalase, glutathione peroxidase activities compared to basal diet-fed fish. EW showed significantly (p < 0.05) better antibacterial efficacy compared to MB. Prophylactic treatment showed better results compared to therapeutic treatment. Bioassay-guided chromatography and GC-MS analysis revealed the presence of hexadecanoic acid, phytol, cis, cis, cis-7,10,13-hexadecatrienal in EW and α-tocopherol, 1-(+)-ascorbic acid 2,6-dihexadecanoate, 1-heptatriacotanol in MB, which was reported to have antibacterial, antioxidative and immunostimulatory potential. Present results indicate prospective use of EW as a prophylactic immunostimulant and effective therapeutic remedy against Aeromonas in Nile tilapia.

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Microbe–Host Metabolic Interaction: Probiotic Approach

Parvathy

Mallick

Unpaprom

et al. 2022

Microbial Engineering for Therapeutics

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Effects of dietary supplementation of vitamin-E and commercial probiotics on the innate immunity of Labeo rohita against Aeromonas hydrophila infection

Cited by 6 publications

References 48 publications

Effects of Vitamin C and E Supplementation on Growth, Fatty Acid Composition, Innate Immunity, and Antioxidant Capacity of Rainbow Trout (Oncorhynchus mykiss) Fed Oxidized Fish Oil

Effects of Vitamin C and E Supplementation on Growth, Fatty Acid Composition, Innate Immunity, and Antioxidant Capacity of Rainbow Trout (Oncorhynchus mykiss) Fed Oxidized Fish Oil

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

Microbe–Host Metabolic Interaction: Probiotic Approach

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