Effects of Different Colored LEDs on the Enhancement of Biologically Active Ingredients in Callus Cultures of Gynura procumbens (Lour.) Merr.

Abstract

Conventional fluorescent lamps that are used in tissue culture are costly light sources, exhibiting excessive wavelength emission-bandwidth that must be replaced by alternative, less costly, and much lower power-consuming energy sources. The use of Light-Emitting Diodes (LEDs) is the best option due to their potential role as elicitors of secondary metabolite production in many plant models. Gynura procumbens (G. procumbens) is widely used for treating various diseases. Here, leaf explants were cultivated in MS medium that was supplemented with 0.5 mg/L of naphthaleneacetic acid (NAA) and 2.0 mg/L of benzylaminopurine (BAP) for 30 days under white, blue, and red LEDs. Secondary metabolites were analyzed by High Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS). Blue LEDs elicited the highest antioxidant activity, total flavonoid, and phenolic content. Furthermore, the content of cyanidin-monoglucosides significantly increased under blue light.

Promising Biomolecules with High Antioxidant Capacity Derived from Cryptophyte Algae Grown under Different Light Conditions

“…Since studies on the effect of light quality on algal PC are rare, we compare our results with certain studies on PC of plants. PC extracted from Gynura procumbens was higher under blue light than white light [ 72 ], similar to our results of C. ozolinii , C. pyrenoidifera , and C . sp.…”

Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects

“…The exceptionally low total phenolic content under white LED treatment for PLBs pre-treated with white LED, suggests that PLBs induce more of these phenolic compounds in order to counteract with the altered illumination sources. The engagement of LEDs as elicitors for secondary metabolites production has been demonstrated in the study conducted by Lian et al [ 56 ]. Narrow light spectra generated by LEDs serve as the abiotic stress factor and trigger the activation of plant defence mechanisms, which in turn leads to the accumulation of secondary metabolites [ 57 ].…”

Light quality and quantity affect graft union formation of tomato plants

“…The utilization of blue LED lights on in vitro callus cultures of Gynura procumbens (Lour.) Merr induced a high antioxidant activity and enhanced the accumulation of total phenol content (TPC), total flavonoid content (TFC), and total anthocyanins TAC 45 . The red-light condition is strong of the most significant biomass aggregation, while violet light condition invigorated the most extreme phenolic and flavonoid blend in callus culture.…”