Effects of different fuchsin analogs on the Feulgen reaction.

Teichman, J.; Krick, Thomas P.; Nettleton, G. S.

doi:10.1177/28.10.6158535

Cited by 11 publications

(8 citation statements)

References 12 publications

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“…These variations are quite troublesome in the exacting technique of biological staining" (Scanlan 1935). More recently, fuchsine samples haves been analyzed using a variety of methods, e.g., mass spectrometry (Teichman et al 1980). A quicker and cheaper qualitative analysis can be performed using thin layer chromatography.…”

Section: Discussionmentioning

confidence: 99%

Quirks of dye nomenclature. 4. Fuchsine: four shades of magenta

Cooksey

2015

Biotechnic & Histochemistry

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Fuchsine, also called magenta, was the second coal tar dye to be produced after mauveine. Fuchsine is composed of a mixture of up to four triphenylmethane dyes that differ only in the number of substituent methyl groups. Unlike mauveine, fuchsine still is widely used today as a biological stain. We describe the progress of fuchsine from its birth as the second coal tar dye, through a variety of modes of manufacture and industrial application, to its current use. We discuss complexities of nomenclature and identification, and the hazards and risks of its various applications.

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Section: Discussionmentioning

confidence: 99%

Quirks of dye nomenclature. 4. Fuchsine: four shades of magenta

Cooksey

2015

Biotechnic & Histochemistry

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“…These dyes differ in the degree of methylation oftheir aminophenyl groups (37) and stain with different intensities when prepared from different batches. Teichmann et al (9) further report that it has been determined that the complex formed by pararosanilin-Schiff's reagent and hydrolyzed DNA has a lower absorption rate than do corresponding complexes formed by Schiff's reagents prepared from magenta I1 or new fuchsin. In the present work, an "impure" Schiff's reagent prepared from basic fuchsin was compared with a "pure" reagent prepared from pararosanilin.…”

Section: Discussionmentioning

confidence: 99%

“…Both Teichman and colleagues (9) and others (35,36) report that such basic fuchsins are variable mixtures of four triaminotriphenylmethane dyes: pararosanilin (magenta o), rosanilin (magenta I), magenta 11, and new fuchsin (magenta 111). These dyes differ in the degree of methylation oftheir aminophenyl groups (37) and stain with different intensities when prepared from different batches.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction.

Kiss¹,

Salmon²,

Camby³

et al. 1993

J Histochem Cytochem.

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We investigated the parameters that could affect the cytophotometric analysis of cell nuclei stained by the Feulgen reaction. These parameters included: the hydrolysis temperature (in the normal "room temperature" range); the composition of the S C W s teagent; the speed of centrifugation of the cell suspensions; the mode of preservation [air-drying or ethanol-formalin-acetic acid (EM) fmtion]; the fmuon time; the pronase digestion time; and the concentration of pronase used to obtain cell suspensions from archival (formalin-fired, pataffin-embedded) materials. Relatively homogeneous material was studied: the MXT mouse mammary adenocarcinoma growing in vivo as tumors with both small and hyperchromatic cell nuclei and in vitro as monolayers with larger and less hyperchromatic cell nuclei. The results of these investigations demonstrate the necessity for the precise definition of a protocol for such procedures

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“…Conditions of the reaction were adapted for densitometric examination using computerized image analysis, which was applied in order to estimate the animal genome sizes (Teichman et al 1980;Greilhuber and Temsch 2001;Hardie et al 2002). A measuring program Cytophotometer for Microscan was used to determine C-DNA values in cell nuclei.…”

Section: Methodsmentioning

confidence: 99%

Genome size of the European domestic goose (Anser anser domesticus)

Andraszek¹,

Wójcik²,

Grużewska³

et al. 2009

Can. J. Anim. Sci.

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2009. Genome size of the European domestic goose (Anser anser domesticus). Can. J. Anim. Sci. 89: 449Á455. This work is aimed at determining the C-DNA contained within the nuclei of different types of cells in the domestic goose Anser anser. Cells from the lungs, skin, pancreas, kidney, spleen, liver, heart, brain, blood, ovary and testicle were analysed. Cells from the blood, ovary and testicle were smeared onto microscopic glasses, whereas slides from the other organs and tissues were prepared using the paraffin technique. DNA content, as visualized by the Feulgen reaction using computerized image analysis, was examined in9200 nuclei of every type of cell. Chicken erythrocytes were used as reference material. Different concentrations of chromatin within cell nuclei were observed, from small, dispersed clods to an entirely filled nucleus surface. It was stated that the average C-DNA content in the domestic European goose amounts to 1.30690.327 pg, which gives goose DNA a length in base pair of 1.277 )10 9 9 0.320 )10 9 bp after adjustment. The correlation between nucleus size and the C-DNA content was positive and high. In all cell types it exceeded 0.6. The highest was observed in lung and ovary cells, the lowest in skin and the pancreas. The majority of all cells (57.34%) contain DNA at the range between 1.0 to 1.5 pg, especially those from erythrocytes and the pancreas (82 and 76% respectively). Liver cells demonstrate a tendency toward an amount that is higher than 1.5% of the DNA (78.61% cells). Heart cells reveal a tendency downward (98.99% below 1.5 pg.). Less than 1.0 pg of DNA was observed in 17.13% of all examined cells.Key words: Domestic goose, cell, cell nuclei, Feulgen reaction, genome size, DNA mass Andraszek, K., Wo´jcik, E., Gruz : ewska, A. et Smalec, E. 2009. Taille du ge´nome de l'oie cendre´e d'Europe (Anser anser domesticus). Can. J. Anim. Sci. 89: 449Á455. Ce travail devait pre´ciser la nature de l'ADN-C pre´sent dans le noyau de diverses cellules de l'oie domestique (Anser anser). Les auteurs ont analyse´les cellules des poumons, de l'e´piderme, du pancre´as, des reins, de la rate, du foie, du caeur, du cerveau, du sang, des ovaires et des testicules. Celle du sang, des ovaires et des testicules ont e´te´examine´es par frottis sur des lames de microscope, alors que celles des autres organes et tissus ont e´te´pre´pare´es avec de la paraffine. La concentration d'ADN a e´te´e´tablie par la re´action de Feulgen suivie d'analyse de l'image sur ordinateur pour9200 noyaux de chaque type de cellule. Les e´rythrocytes de poulet ont servi de point de re´fe´rence. La concentration de chromatine varie dans le noyau, allant de petits amas disperse´s a`un noyau entie`rement plein en surface. On estime que la teneur moyenne en ADN-C se situe a`1,30690,327 pg chez l'oie, ce qui donne 1,277 )10 9 9 0,320 )10 9 paires de bases apre`s correction. La taille du noyau et la concentration d'ADN-C pre´sentent une forte corre´lation positive. Cette corre´lation de´passe 0,6 dans toutes les cellules. La plus for...

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Effects of different fuchsin analogs on the Feulgen reaction.

Cited by 11 publications

References 12 publications

Quirks of dye nomenclature. 4. Fuchsine: four shades of magenta

Quirks of dye nomenclature. 4. Fuchsine: four shades of magenta

Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction.

Genome size of the European domestic goose (Anser anser domesticus)

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