Effects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon (Citrullus lanatus L.)

Abstract

15Two watermelon genotypes, one commercial watermelon variety (Ustun F1) and five 16 different doses of gamma rays coming from Co 60 were tested to develop useful haploidization 17 procedure in watermelon. For this purpose, male flowers collected a day before anthesis were 18 irradiated with 50, 150 , 200 , 275 and 300 Gy doses of gamma ray and female flowers were 19 pollinated with irradiated pollen the next day. Seeds extracted from fruits harvested 25 days later 20 were opened individually in a laminar flow hood. Embryos obtained via embryo rescue technique 21were placed in glass tubes containing CP medium with 30 g l -1 sucrose, 8 g l -1 agar, 0.08 mg l -1 22 B12, and 0.02 mg l -1 IAA. Sixty haploid embryos were obtained from 43 watermelon fruits in 23 this study. Genotype 1 was found to be the most successful genotype with 3.57 haploid embryos 24 per 100 seeds. Among tested irradiation doses, 275 Gy was better than other doses with 5.26 25 haploid embryos per 100 seeds. Considered together with irradiation dose and genotypes, the 26

Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits

“…The plant material usually decreases progressively during each step of the DH process, due to mortality, inefficiency of the method and recalcitrant performance. Frequently, the ratio of parthenogenetic embryos per fruit is low, 0.23-5.79 in cucumber [9,10]; 0.2-16 in pumpkin, squash and winter squash [8,[11][12][13][14]; 0.3-6 in melon [3,6]; or 1.4 in watermelon [15].…”

Embryo rescue technique and its applications for seedless breeding in grape

“…The culturing of cherry embryos in 1933 by Tukey (1933) represents a milestone in the embryo culturing of fruit crops. To date, embryo rescue has been widely used in many fruit crops, including apple (Dantas et al 2006;Druart 2000), banana (Bakry 2008;Uma et al 2011), citrus (Viloria et al 2005;Xie et al 2013Xie et al , 2014, mango (Krishna and Singh 2007), muskmelon (Ezura et al 1994;Nuñez-Palenius et al 2006), peach (Anderson et al 2002;Pinto et al 1994), persimmon (Hu et al 2013;Leng and Yamamura 2006;Yamada and Tao 2007), and watermelon (Taşkın et al 2013), etc. for various purposes such as seedless breeding, triploid breeding and interspecific breeding.…”

Modification of centromere structure: a promising approach for haploidline production in plant breeding

“…A common method for the production of haploid lines relies on the regeneration of microspores through anther culture (Taşkın et al, 2013a(Taşkın et al, , 2013bBaktemur et al, 2014;Olszewska et al, 2014). A second common way is through interspecific hybridization, in which a domesticated crop is crossed to an inducer line to generate haploid offspring (Kasha and Kao, 1970;Barclay, 1975;Savaskan et al, 1997;Chaudhary et al, 2005).…”